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Two days after bone marrow MSCs compounded to nano-CS/COL scaffold, bone marrow MSCs presented globular shape and were scattered; Four days later, bone marrow MSCs presented shuttle shape, extended and anchored on the surface of nano-CS/COL by pseudopods; Eight days later, bone marrow MSCs proliferated and fused each other, and they secreted a lot of extracellular matrix, then which covered most material particles.

骨髓基质干细胞复合到纳米壳聚糖-胶原纤维支架后2 d,细胞呈球形散在分布;4 d 后细胞呈梭形,延展爬行且有伪足与材料表面锚靠;8 d 时细胞增殖,相互间融合,并有大量的细胞外基质分泌,大部分材料颗粒被覆盖。

This gene, together with a phytase gene ( 168phyA) identified in the B. subtilis strain 168 genome by a homology search, was cloned and over-expressed in B. subtilis using a phi105MU331 prophage vector system. Up to 35 units of phytase/ml were secreted into the culture media; and mature enzymes of around 44-47 kDa were purified for characterization.

借助于ф105MU331前噬菌体载体系统,这个基因和另一个通过同源性研究并在B.subtilis菌株168的基因组中被鉴定的植酸酶基因(168phyA),于B.subtilis中得到克隆和过度表达。B.subtilis菌体向培养基中分泌了高达35个单位的酶夜,同时分子量在44-47kDa之间的成熟酶得到纯化。

Some extracellular proenzymes may be activated by secreted lysosomal proteinases.

有些细胞外酶原能被分泌的溶酶体蛋白酶所激活。

MMPs are usually secreted as latent, inactive proenzymes, which need to be activated (Fig 1 ), and/or are bound to their native inhibitors, the tissue inhibitors of metalloproteinases.8 Activation may involve proteolysis and/or oxidation of the latent enzyme.8 Proteolytic activation of the proenzymes cleaves the propeptide sequence, which masks the active site of the latent enzyme.

金属蛋白酶常以无活性的酶原方式分泌,而后者要么需要被激活要么与附近的或组织中金属蛋白酶抑制物结合。激活作用包括了具有潜在酶的蛋白水解作用或氧化作用。酶原的蛋白水解激活作用能将掩盖活性部位的潜在酶的前肽序列破坏。

Acne occurs most often to teens and preteens because these androgens are initially secreted at the onset of puberty.

痤疮最常发生于青少年和福音,因为这些都是男性贺尔蒙分泌最初在青春期开始。

In the first cultivation stage,as relatively high concentration of phosphorus,phosphorus from water column was removed to a certain extent by periphyton.The end of the incubation,as low concentration of phosphorus periphyton releases phosphorus to water column instead of removal phosphorus from water,this may closely relate to the alkaline phosphatase secreted by periphyton in water body.

从培养过程中水体磷的浓度变化来说,培养初期,水体中磷的浓度比较高,附着生物对其有一定的去除作用;培养后期,水柱中磷的浓度比较低,附着生物不但不去除水中的磷,而且还向水柱中释放磷,这可能与附着生物向水体分泌碱性磷酸酶密切相关。

Second,we set the database of morphological characteristics of intercepted and captured bark beetle during quarantine,and the last,we studied the molecular identification of 15 species.The results were showed as below.1.Application of bark beetle semiochemicals in timber quarantine1.1 EAG and walking responses of bark beetle to different semiochemicalsSemiochemicals which can induce the olfactory response and aggregation behavior of bark beetle include host volatile components,pheromones secreted by bark beetles itself or synthesized with host compounds by bark beetles,and the compounds released by tissue of decaying wood under the pressure of microorganism after bark beetle colonization.

信息化合物在小蠹虫检疫中的应用1.1小蠹虫对各种信息化合物的触角电位及嗅觉反应能诱发小蠹虫产生嗅觉反应,进而使之产生聚集行为的信息化合物有很多种,主要包括:树木的挥发性组分;小蠹虫自身产生的信息素和寄生后转化寄主化学物质合成的信息素统称虫体信息素以及树木被小蠹虫寄生后,其组织在微生物作用下释放的物质。

This culture method should be of great value in somatic cell gene therapy for the delivery of secreted gene products to the plasma of large mammals.

这种文化方法应该有在为到大的哺乳动物的血浆的隐藏的基因产品的交货的肉体的房间基因治疗的伟大的价值。

METHODS Interferon-γ and interleukin 4 (IL-4) secreted by CD 3 +, CD 8 -T cells were detected by flow cytometry in 38 patients with secondary syphilis and 20 healthy volunteers, and significance of TH1/TH2 cell ratio in the etiology of syphilis was analyzed.

采用流式细胞分析技术检测 38例Ⅱ期梅毒患者和 2 0例健康体检者外周血CD3 +、CD8-T细胞胞内细胞因子IFN γ和IL 4的表达。

The fulllength heparanase gene is isolated. The functional heparanase is expressed within COS7 and CHO cells and few heparanse is secreted into medium. The positive CHO cells expressing active heparanase is screened, which will help explore its function and screen its antibody and antagonists from a variety of sources.

本研究分离出人HPA编码基因全序列;在真核细胞内成功表达出有活性的人HPA,发现此酶在COS7和CHO细胞中表达在细胞内,很少分泌到细胞外;筛选出其恒定表达细胞株,为进一步研究HPA结构功能、开发其抗体及抑制剂提供了有力的工具。

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