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To investigate the methods to effectively and simply assess the CAG repeat size of HD gene which was necessary for gene diagnosis of Huntington disease, the sequence including polymorphic CAG repeat of HD gene was amplified by PCR with TaKaRa LA Taq DNA polymerase and GC buffer. PCR products were analyzed on polyacrylamide gel to distinguish normal alleles from HD alleles. The DNA fragments of affected alleles were recovered from polyacrylamide gel as templets for secondary PCR. The secondary PCR products were cloned into T vector for sequencing analysis to determine CAG repeat size. A total of 20 normal individuals and 3 members from a HD pedigree were included in this study.

为了简单高效检测HD基因开放阅读框5'端n三核苷酸重复序列，建立快速准确的亨廷顿病(Huntington disease， HD)基因诊断方法，应用TaKaRa LA Taq DNA聚合酶配合GC buffer扩增HD基因包含n重复序列的目的片段，非变性聚丙烯酰胺凝胶电泳检测后回收n拷贝数异常增多的目的片段，再次PCR扩增后将产物连接至T载体，进行DNA测序确定CAG的拷贝数。

The result showed that the surface architecture and ultrastructure of I.punctatus ' gill and secondary gill lamellae were similar to those of other teleosts. The surface of gill arches and gill rakers was covered with pavement cells which were characterized by circular microridges, and secreting-holes were found among cells on the surface of gill arches. The base part of gill rakers were bigger in terms of diameter. The pavement cells' surface of gill filaments' middle part was characterized by microridges with fine lateral protuberances on both sides which were either fused or interruped, while the microridges disappeared on the pavement cells' surface on both top and base parts of gill filaments, forming dense granulated protuberances. The height and interlamellar space of secondary gill lamellae in I.punctatus were relatively higher and bigger, respectively.

结果表明，斑点叉尾鮰鳃的表面结构和微细结构与其他硬骨鱼类基本相似，鳃弓和鳃耙表面被具环形微嵴的扁平上皮细胞所覆盖，鳃弓表面细胞之间有孔洞；鳃耙基部较粗壮；中部鳃丝上皮细胞表面的微嵴两侧有细小的横突，有些微嵴出现融合或间断；端部和基部鳃丝上皮细胞表面的微嵴消失，形成密集颗粒状突起；鳃小片的高度和片间距较大。

The results showed that there were three major types of secondary forests, Fungshui wood, ravine wood, and early stage of secondary forest. Fungshui wood and Ravine wood had higher floristic diversity and are the precious forest relicts.

研究结果表明，珠海市山地森林群落类型主要有风水林、沟谷林和早期次生林，以风水林和沟谷林的物种多样性较高，它们是珠海市宝贵的森林遗产。

Datong children of railwaymen's Secondary School, located in Datong railway Xinhua A Backstreet One is the Beijing Railway Bureau Datong-owned railway sub-administration of a full secondary school.

大同铁路职工子弟第二中学，位于大同铁路新华后街甲一号，是北京铁路局大同铁路分局所属的一所完全中学。

We opened the campus radio, teacher Zhan Ming-ying, and broadcasters, correspondents dedicated the beautiful audio-visual works with perseverance to everyone; we have compiled and published our own newspaper,"Journal of LQ Middle School"; our blackboard newspaper, and cultural fields are more standardized and regular ; we have organized the celebration of "Women's Day" and Teacher's Day, We organized faculty members to go out for inspection, carried out teachers and staffs Red song concerts; we held exchange activitives with No.5 Middle School of Anqing, Changling secondary schools, Taihu Dashi secondary school, LQ primary school, Bohu primary school; we held a school-based training courseware, organized all the teachers listened to local experts, Yang Jiedong's thematic reports, teacher Wei Shusheng's video report, organized the head teachers to listen to the national top video of the head teacher report , organized the teacher to Nanjing and Shanghai and Anqing Teachers College in groups for refresher training, seriously at all levels of teaching and research activities; officially formed a faculty basketball team and on behalf of LQ village took part in the county's National Day competition; teacher Zhu Hongmin and Xu Chunming represented our school to take part in "springs Cup" Table Tennis competition; we brought in the dance team of the county college for the old to perform; we held the graduation ceremony of the 2009 session; we hold regularly the reading and speaking contest for students, the English showing, the teachers Courseware competition, young and middle-class high-quality math and English classes Contest quality; we hold table tennis competition, successfully held the second session of the school track and field sports; General League branch, and students' union held activities to commemorate the earthquake in Wenchuan and commemorate teacher Li Jinhua , has organized fund-raising activities for many times; branch of the Praty, Representatives of the staffs, General branch of the League, and students union held a successful general election; Organized CDC of county and township hospitals for free medical examinations for students, extensively carried out the publicity of combating TB and the prevention and control and we have safely passed in 2009!

我们开通了校园广播，詹铭瑛老师和播音员、通讯员持之以恒地把精美的视听作品奉献给了大家；我们编辑出版了自己的报纸《凉泉中学报》；我们的板报和文化栏更加规范化、经常化；我们举办了三八节和教师节的庆祝活动，组织了教职工外出考察，开展了教职工红歌演唱会；我们举行了与安庆五中、长岭中学、太湖大石中学、凉泉小学、泊湖小学的校际交流活动；我们举行了校本课件制作培训、组织全体教师聆听了本土专家杨结东主任的主题报告、魏书生老师的录像报告、组织了班主任老师聆听全国十佳优秀班主任的录像报告、分批组织了老师到南京到上海到安庆师范学院进修培训、认真参与各级教研活动；正式组建了教工篮球队并代表凉泉乡参加了全县国庆比赛；朱红敏和徐春明老师代表学校参加了凉泉乡五四&清泉杯&乒乓球比赛；我们请来了县老年大学的舞蹈队来校演出；我们隆重举行了2009届毕业典礼；我们定期举行了学生读书演讲比赛、英语风采大赛、老师课件制作比赛、中青年数学优质课和英语优质课大赛；我们分校区举办了全校乒乓球比赛，成功举办了第二届全校田径运动会；团总支和学生会举行了纪念汶川大地震的活动、纪念李精华老师的活动、多次组织了募捐活动；党支部、教代会、团总支和学生会成功举行了换届选举；组织县疾控中心和乡卫生院免费为学生体检，广泛开展了防治结核病和防控甲型流感的宣传，平安地度过了2009年！

By consideringthe formation time of secondary particles we have developed theparticipant nucleon model incorperating the secondary collisionsnaturally and calculated the increase of the 〓 ratio caused by thesecondary collisions of pions with target nucleons.

本论文另一研究工作就是通过考虑次级粒子的形成时间，自然地将参加者核子模型发展成为包括次级碰撞过程的模型，并根据此物理图象，计算了高能原子核一原子核碰撞过程中由于π介子与核子的次级碰撞过程而导致的次级粒子中的K+/π+比例的增加。

For example, some primary and secondary copper smelting plant is still in use behind blast furnace process, sulfur dioxide utilization rate of only 65%; primary and secondary lead smelting enterprises, commonly used sintering pot lead smelting process, flue gas concentration is low, can not use the sulfur into the atmosphere each year up to 25 million tons.

例如，一些中小学炼铜厂仍处于落后高炉使用过程中，二氧化硫的只有65％的利用率，小学和中学铅冶炼企业，普遍使用的烧结锅炼铅工艺，烟气浓度低，可不使用到大气中的硫，每年达到25万吨。

The First Part discusses the rationale of cultivating the maths self-learning ability of secondary vocational school students, making an overall theotvlical explanation in the urgency and possibility of cultivating the maths self-lenmmg ability of secondary vocational school students, the learning and teaching features of ninths self-learning ability and the teaching principles of maths self-learning ability cultivation.

第一部份，讨论培养中职学生数学自学能力的理论基础，从培养数学自学能力的迫切性、可能性，数学自学能力的学习特征，教学特征，以及数学自学能力培养的教学原则等方面作了较为全面的理论阐述。

The air is divided into primary air, secondary air, burning rice husk, the one, secondary air ratio of 50:50, a cold wind from the furnace chamber 2 side of the water entering through the fabric wind deflector hood holes into the combustion chamber.

空气分为一次风、二次风，燃烧稻壳时，一、二次风之比为50：50，一次风从炉膛水冷风室二侧进入，经布风板风帽小孔进入燃烧室。

According to the boiler burner design features introduced secondary air baffle according to their works were divided into secondary air baffle, baffle perimeter wind, burn the top layer of the wind baffle and oil damper baffle gun.

根据锅炉燃烧器地设计特点，推荐二次风挡板根据其起作用不同分为辅助风挡板、周界风挡板、顶部燃尽风挡板和油枪层风门挡板。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。