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The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因,并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列,通过对克隆的降钙素序列的比较研究,结果显示同一科的鱼降钙素序列保守性较高,同时,根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究,结果显示在分类学上,分类地位较远的鱼,其降钙素相似性较低,分类地位越接近的鱼,其降钙素相似性越高;我们利用谷胱甘肽S-转移酶(Glutathions S-transferase,GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究,应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索,并将纯化的融合蛋白作为抗原,获得了高效价的兔抗鲑鱼降钙素免疫血清;生物活性研究表明,大肠杆菌表达的融合蛋白具有显著的降血钙作用。

The company ground Pearl Rever Delta the interior region foshan mountain City south china sea area even continent, adjacent Guangzhou,the foshan mountain two sides is apart from south china sea harbor is only 500 meterses, is 15 kilometers apart from train station in Guangzhou, is 50 kilometers apart from new white cloud airport in Guangzhou, geography position with abundant resources.

公司地处珠江三角洲腹地佛山市南海区平洲,毗邻广州、佛山两地距南海港仅500米,距广州火车站十五公里,距广州新白云机场五十公里,地理位置得天独厚。

And you, lift up your staff, and stretch out your hand over the sea, and divide it; and the children of Israel shall go into the midst of the sea on dry ground.

14:16 你举杖向海伸手,把海分开;以色列人要下到海中走干地。

We want to buy Sea Shells, Sea Cucumbers, Gum Arabic, Honey, Pigeon Peas.

我们要采购海洋贝壳,海参,安拉伯胶,加蜜,鸽子豌豆。

In the night as flooding tide of sea ,i feel the slightly gurgitation , it's too dark to see the far destination .,i believe you as believe the box and needle of the sea

这个大海般涨潮的夜晚,我可以感觉到它微微的起伏。黑暗中看不到远方的目的地,我信任你,如同信任海上的罗盘。

It is found that there are significant seasonal and interannual changes in the warm pool. The development of South China Sea warm pool is closely related to the gyre activities in the sea and imported warm water from Indian ocean besides radiative warming.

结果表明,南海暖池有显著的季节变化和年际变化;暖池的发展除与太阳辐射有关外,还与海洋中涡旋活动及从印度洋输入的暖水有密切关系。

Maoming city hacienda Chemical Co., Ltd. is a joint-stock enterprises, located in the South China Sea Sea grams States in Maoming City.

茂名市先达化工有限公司是一家股份制企业,座落克国南海之滨的茂名市。

Using annual hairtail catch and fishing effort data between 1951 and 1984, as well as time series of precipitation, wind speed, and sea surface temperature, interannual trend and variation of hairtail catch in East China Sea were analyzed and a regression catch model incorporating fishing effort and environmental variables were established.

利用1951~1984年东海带鱼年渔获量和捕捞努力量资料,以及降雨、风速和海表温度等的时间系列,分析了带鱼渔获量年际变化与捕捞努力量及环境因素的关系,并建立了渔获量对捕捞努力量和环境变量的回归模型。

Hairtail the East China Sea, the East China Sea pomfret, crab and so on,"seafood xiangshan" fly under the banner of the first landing in Lanzhou and Lanzhou-ling yesterday to lying Zhuang Hotel in Lanzhou on-site to promote the public.

东海带鱼、东海鲳鱼、梭子蟹等等"象山海鲜"打着飞的首次登陆兰州,并于昨日在兰州宁卧庄宾馆向兰州市民进行了现场推介。

In 2004, for instance, a British royal commission described in bleak detail the collapse of north-east Atlantic cod, of North Sea hake and plaice, and of other species discarded as "bycatch" and thrown back dead into the sea in huge numbers.

例如,2004年,某个英国皇家委员会令人沮丧地详细描述了东北大西洋鳕鱼、北海鳕鱼类和鲽鱼类、其它通常被丢弃的"附属捕获物"和因死亡被抛回大海的物种的巨大数量的毁灭。

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Do you know, i need you to come back

你知道吗,我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。