查询词典 sea perch

The main purpose of this article is to apply the newly developed Parity Bounds model and then compare it with Ravallion model in the market integration research of Taipei and Taichung aquaculture markets. We choose 11 types of fishes including Japanese sea perch, big head carp, milk fish, grass carp, common carp, curcian carp, white mouth croaker, Japanese butterfish, black pomfret, white pomfret, and gold threadfin.

本研究主要在应用新近发展的等值临界模型对台北、台中两地鱼市场中，都有进行交易之七星鲈、大头鲢、草鱼、虱目鱼、鲤鱼、鲫鱼、白口、肉鱼、黑鲳、白鲳、金线等十一种鱼种进行市场整合检定，并与另一种市场整合检定－Ravallion模型比较，希望藉此二种不同方法来了解台北、台中两地鱼市场间是否符合单一价格法则所隐含之市场整合的情形。

The fry of black seabreamand giant sea perch with 4.0cm-8.5cm using the methods of dyeing, i.e., the fish immersed in the water solution containing comestible pigment, immersion in tetracycline, i.e., the fish immersed in the water solution containing tetracycline, and marking by spraying, i.e., the fish sprayed with fluorescent pigment spraying on the skin of fish, and double marking, i.e., the fish sprayed with fluorescent pigment spraying on the skin of fish after feed with tetracycline, and control group. The survival rates, mark retention rates and hydrology data were separately measured every three days for black sea bream and every two days for giant sea perch. The results were as follows.

本研究针对体长4.0cm-8.5cm的黑鲷及金目鲈的鱼苗采用染色法(将鱼只浸泡在含有食用色素的水溶液中)、四环霉素浸泡法(将鱼只浸泡在含有四环霉素的水溶液中)、喷枪法(利用高压喷枪将萤光色素打在鱼的表皮)以及双重标识法（四环霉素投餵法加上喷枪法）与一组空白组进行试验，黑鲷每三天一次、金目鲈每两天一次，调查标识后鱼只的时间别残存率、时间别标识残留率以及水质资料，结果如下。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

It is shown from the detection results that VAMP-2 is expressed in intestine, liver, kidney, skin, spleen and sex gland as well as brain of Japanese sea perch at different levels, but no VAMP-2 is expressed in...

结果表明，除脑组织外，在肠、肝脏、肾脏、皮肤、脾脏、性腺等组织中也检测到 VAMP-2的表达，各组织间的表达水平亦存在差异，肌内组织未检测到表达。

In addition, it has been found from several marine biologies such as zebra fish, trout arid crab. But such research taking sea perch as the material has not been done in the world.

而海洋经济鱼类如鲈鱼，却从未被克隆与研究，本实验就以海洋经济鱼类鲈鱼作为实验材料，对Syntaxin 1B基因进行克隆和序列分析。

The endurance abilities of the embryos of sea perch, flounder and turbot at different developing stages to vitrification solutions were studied. The result showed that sea perch at tail-bud stage, heart-beating stage and pre-hatching stage had more endurance abilities to VSD2, flounder at 4-5 somites, 16-20 somites and tail-bud stage had more endurance abilities to PM3, and turbot at 4-5 somites, 16-20 somites and tail-bud stage had more endurance abilities to PMP1. The feasible equilibration time of three marine fish embryos in vitrification solutions was determined, which guided the dealing process before freezing.

对花鲈、牙鲆、大菱鲆三种鱼类不同时期胚胎分别在玻璃化液中的适应性进行了研究，发现花鲈尾芽胚、心跳胚和出膜前期胚对VSD2的耐受能力较强；牙鲆4-5以肌节胚、16-20对肌节胚、尾芽胚对PM3的耐受能力较强，大菱鲆4—5对肌节胚、16—20对肌节胚、尾芽胚对PMP1的耐受能力较强，较适合于进行玻璃化液处理和冷冻保存；并且确定了三种鱼类各时期的胚胎在玻璃化液中的适宜平衡时间，为胚胎在玻璃化液中处理和冷冻提供了一定的参数。

The Western blot and in-situ hybridization were used to study the expressions of VAMP-2 in different tissues of Japanese sea perch.

采用免疫印迹方法和原位杂交技术，研究了VAMP-2在鲈鱼组织中的表达情况，分别对鲈鱼8 种组织中的VAMP-2进行了检测。

Whole sequence of SNAP-25, eighty percent of VAMP2 and half of Syntaxinl gene were finally cloned from Japanese sea perch.

最终得到SNAP-25基因全长序列，VAMP2基因80％的序列和SyntaixnlA基因50％的序列。

Firstly, morphology, lactate dehydrogenase pattern and random amplified polymorphic DNA fingerprinting of three continuous marine fish cell lines of FG, SPH and RSBF, derived from flounder gill, sea perch heart and red sea ...

本文首先从形态、蛋白质和DNA三个层次水平对牙鲆鳃细胞系FG、鲈鱼心脏细胞系 SPH和真鲷鳍细胞系RSBF的特性进行了分析，并分别与其所对应的原代培养组织作了比较。

This research is taking sea perch as the material, and cloning the Syntaxin 1B gene. After extracting RNA from sea perch tissue, we gained the first chain of cDNA by RT-PCR. Then designing a pair of primers according to the sequence of Syntaxin 1B from other organism. Based on the first chain of cDNA cloned from sea perch, the partial sequence of Syntaxin 1B gene is been cloned by using TD-PCR and we received the 751bp fragment at last.

本实验从鲈鱼脑组织中提取总RNA，通过反转录得到cDNA第一条链，根据已克隆出的其它生物的Syntaxin 1B基因序列，设计并合成引物，在妒鱼syntaxin1B基因部分序列克隆与分析 cDNA第一条链的基础上使用降落PCR，进行体外扩增妒鱼 Syntaxin IB基因的部分序列，得到长度为75 lbP的片断，在胶中洗脱下该片段，与pMD 18一T载体链接，转化入感受态细胞，挑取阳性克隆扩大培养，提取质粒，经HindIH和x一gaH两内切酶消化得到75 1 bp的片断，证明链接成功。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。