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One L-isoleucine high-producing strain ISW330 with genetic marker was screened through shake tube primary screening, shake flask compound screening, genetic marker test and single clone isolation and continuous passage cultivation This strain could produce L-isoleucine 9.18g/L after fermentation under the fermentation condition without being optimized.

以黄色短杆菌HL41为出发菌株，通过硫酸二乙酯和紫外线诱变处理，经摇管初筛、摇瓶复筛、遗传标记验证、单菌落分离和连续传代，筛选出一株L-异亮氨酸生产菌株ISW330(Met~-+AEC~r+α-AB~r)。

One L-glutamic acid high-producing strain TN6319 with genetic maker (FEA~r+KMA~r+SG~r ) was screened through shake tube primary screening ,shake flask second screening,genetic marker test and single clone isolation and continuous passage cultibation.this strain could produce L-glutamic 72.9g/L after fermentation 42h under the fermentation without being optimized.

经摇管初筛、摇瓶复筛、遗传标记验证、单菌落分离和连续传代，最终筛选出一株L-谷氨酸高产菌TN6319(氟乙酸~r、酮基丙二酸~r、谷氨酰胺~r、磺胺胍~r)，该菌株在未优化条件下发酵42h产L-谷氨酸79.2g/L。

The result would be used in tobacco breeding.4 SRAP technology is used to study the genetic differences among different types of tobacco, including flue-cured tobacco, oriental tobacco, maryland tobacco, burley tobacco, and air/sun cured tobacco, five pairs of primer combinations which can amplify characteristic bands has been screened. Among the five combinations, two of them (SF25*SR2 and SF3*SR26) can distinguish air-cured tobacco including maryland tobacco and Burley tobacco from the other cultivated forms; SF20*SR14 can distinguish sun cured tobacco including oriental tobacco and sun-cured tobacco; SF16*SR14 can distinguish flue-cured tobacco and air-cured tobacco, and SF22*SR14 can distinguish DBJ599 with specisl aroma and the other flue-cured tobacco.5 8 varieties , which represent the different types of tobacco, are divided into 3 groups. I: G-28, II:DBJ599、MD40、By21, III: Basma、Sumsun、XNS、QCT. The cluster analysis result es consistant with experience evatuation.6 Anorthogonal design was used to optimize a ILP-PCR system with 5 factors (DNA, Mg2+, dNTP, primer and Taq polymerase) at 4 levels.The result showed the optimized ILP-PCR system for Nicotiana tabacum L. was: 2uL10×PCRbuffer, 20ng template DNA, Mg2+ 2.0mmol/L, dNTP 150umol/L, primer 0.3umol/L, Taq DNA polymerase 1 U in a total of 20uL reactionsolution.

研究结果可作为烤烟与香料烟类型间的杂种鉴定利用的实验与理论依据。4、利用SRAP标记技术，研究和分析了烤烟、晒烟、晾烟、白肋烟、香料烟等不同栽培类型间的基因型差异，初步筛选出了能够在分子水平上反映不同烟草类型差异的特征性标记带：晾烟MD40、Ky17、Burley21、TN90基因型共有的特征带有两对引物组合，分别为SF25*SR2和SF3*SR26；晒烟小牛舌、青梗、小花青和香料烟Sumsun、komotini Basma、Xanthi Basma共有的特征带引物组合为SF20*SR14；NC89、K326、G-28、大白筋和MD40、Ky17、Burley21、TN90共有的特征带引物组合为SF16*SR14，烤烟中大白筋599的特征带引物组合SF22*SR14.5、利用61对SRAP引物组合，研究了普通烟草不同类型间的亲缘关系，将普通烟草类型间8个不同供试材料划分为3类别。I：烤烟类型G-28II：白肋烟类型(MD40、By21、大白筋599III：香料烟类型(Basma、Sumsun、小牛舌、千层塔)。

In order to make the right evaluation of the state which is the consumption consume of the steam line system, we make an test of the heat loss of the steam line network. And we also probe into the heat loss of the steam line system and the factors. In order to solve the heat inefficency of the steam line system, we look into the present situation of the steam line network and the state of heat preservation. Then we found the open questions and the weak points of heat preservation. We make the evaluation and affirm the reason of the heat inefficency of the system. We adopted the combination of bench test and engineering optimization, then we screened out high-quality adiabator, and designed the best heating composition. We designed and developed the software"The analysis and optimization of energy of the steam line system in thermal production" It can offer convenience to the computational analysis of heat loss of the steam line system. It can also be used for design calculation and experimental verification of steam pipeline feasible radial-radius, so it can offer technical support for the reduce of heat loss of the system, the raise of well head steam quality and the raise of heat efficicency of the steam injection system.

为了对输汽系统的用能状况作出正确的分析评价，对输汽管线热损失进行了测试计算，对输汽系统热量损失及影响因素进行了探讨；为了解决输汽系统热效率低的问题，从输汽管网现状及管道保温状况调查入手，掌握存在的问题和保温薄弱环节，做出分析评价，确认导致系统热效率低的原因，采用室内试验与工程优化相结合的办法，筛选出优质保温材料，设计最优保温结构，同时设计开发了&热采输汽系统能量分析及优化软件&，为输汽系统热量损失计算分析和保温结构优化提供了方便，还对输汽管线合理辐射半径进行了设计计算及实验验证，从而为降低系统的热能损失，提高井口蒸汽干度，提高注汽系统的热效率提供了技术支持。

Two halophilic and alkaliphilic strains screened out from the soda lake in the Hulunbeir area of Inner Mongolia were studied for alkaline pectinases activity.

本论文所用菌种是从内蒙古海拉尔地区的碱湖中筛选得到两株产碱性果胶水解酶的嗜盐嗜碱菌。

Four beagle dogs with sinus arrhythmia were strictly screened from 200 with 15%incidence rate of SA.They were divided into two groups(6g and 3.75g crude drugs/kg,bid ,for 17 successive days).

20 0条Beagle犬中窦性心律不齐的发生率为 1 5%，从中严格筛选和甄别 4条窦性心律不齐Beagle犬，观察心脉安颗粒剂( 6g生药/kg ，3 。75生药/kg ，Bid)连续给药 1 7d的影响作用。

It is learnt that jianeng always include headquarters years pin 10% set aside for the development of a unique solution to the cost of technology. 1991 to 1995, the United States patent registration number 10 companies, Canon screened-in the top five, but IBM does not divide the Chung-edmuncl.

据悉，佳能总是将总部年销额的10%拨为开发独创技术的费用。1991年至1995年，美国专利注册件数前十位公司，佳能入围前五名，而且与IBM不分仲伯。

Based on the self-making standards, the study screened five children who had serious problems, and comparing the scores of the questionnaires before and after the investigation.

第三部分是个案箱庭干预的结果与分析。本研究从箱庭的创伤主题、治愈主题和转化主题等三个维度进行分析。

By using the teachers'questionnaire and the Rutter child behavior meter, the study investigated large, medium and small three classes of the kindergarten in Dalian.Based on the self-making standards, the study screened five children who had serious problems, and comparing the scores of the questionnaires before and after the investigation.

主要采用教师提名法和Rutter儿童行为量表对大连市某幼儿园的大、中、小三个班的幼儿进行了儿童行为问题的调查问卷，设定筛选标准，筛选出问题较严重的5名幼儿，并对5名幼儿的行为问卷量表得分进行了前后测比较。

10 Screened from 19 rice varieties were used to study the biological characteristics of rice root border cell s and their toxicity responses to ferrous iron stress.

以水稻为实验材料，从19个品种中筛选出来相对耐亚铁的水稻品种中优9288和敏感品种汕优10号，研究水稻根边缘细胞的生物学特性及对铁毒的响应。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。