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Nested PCR analysis and southern hybridization analysis showed that no polymorphism between H.villosa, Pm97034 and susceptible parent Wan7 107 was detected with the clones from 6VS arm, whereas three clones from H.villosa genome DNA: RH42, RH55, and RH66 showed polymorphism. RGA6 cloned from H.villosa genome DNA was characterized identity to NBS, and show high homology to resistance genes as RPM1 and RPP13 in Arabiadopsis, LRR19 in wheat, and I2C-1 in tomato.cDNA library constructed from T.aestivum-H.villosa translocation line Pm97034 was screened by hybridization with RGA6. Four positive cDNA clones were obtained. R3-2-2 showing 60-90% identity with eukaryotic sulfite oxidases contained an open reading frame of 647bp encoding 140 amino acid, and contained a conserved Moco-dimer domain in the ORF. R6-2-2 showed an ORF containing an Euk-porin domain of 279aa with 20-40% identity to the eukaryotic voltage-dependent anion channel proteins. R8-1-1 showed a complete ORF of 1810bp encoding 493aa with 80-90% identity to plant catalases. R9-1-1 showed an ORF containing a BTB/POZ conserved domain of 204aa with 30-70% identity to pox virus and zinc finger proteins. R3-2-2 and R9-1-1 were the first cDNA clones containingconserved domain of SO and POZ respectively isolated from wheat. R8-1-1 contained a complete ORF with 81% identity to Cat-3. Aspects of the role of R8-1-1 may be same with Cat-3, and it would offer the opportunity for improvement of stress tolerance of wheat.

以RGA6为探针，筛选用抗白粉病小麦—簇毛麦易位系Pm97034构建的cDNA文库，得到了4个阳性克隆。R3-2-2与动植物的亚硫酸氧化酶(sulfite oxidase，SO)有60～90％的同源性，长度为647bp，编码140个氨基酸，具有开放阅读框并含有保守域Moco-dimer.R6-2-2与真核生物的VDAC(voltage-dependent anion chennel)蛋白有20～40％的同源性，长度为1047bp，编码279aa，是一个不完整的开放阅读框，预测结构具有Euk-porin结构域。R8-1-1与植物中已经克隆的过氧化氢酶有80～90％的同源性，长度为1810bp，编码493aa，具有完整的开放阅读框和过氧化氢氧化酶保守域。R9-1-1与动植物的POZ(pox virus and zinc finger protein)蛋白有30～70％的同源性，长度为1446bp，具有开放阅读框和BTB／POZ保守域。R3-2-2与R9-1-1是首次从小麦中克隆到的具有SO和POZ保守域的cDNA序列。R8-1-1具有完整的开放阅读框，与玉米Cat-3基因具有81％同源性，预测R8-1-1可能具有与Cat-3类似的功能，可为转基因小麦抗逆育种提供新的基因。

Like Titian,Poussin sets the figures against a distant background ,partly screened by trees ,of sky and airy blue vistas .

像提香一样，普桑把画中人物置身于一个部分被树木遮蔽的遥远的背景中，由天空和清薄的蓝色深景构成。

Potato flour, soybean powder, FeSO_4 were screened out as the most suitable carbon source, nitrogen source and inorganic salt source, respectively. Based on these, screening methodology Plackett-Burman design was used to evaluate the effects of twelve factors related to amylase production and three statistically significant factors soybean powder, temperature, bran were selected.

酶对底物的作用方式表现出多样性，以表面扩散侵蚀的方式作用于马铃薯和木薯生淀粉，以表面扩散侵蚀和形成大侵蚀孔洞的方式同时作用于红薯淀粉。4、利用响应面法对菌株AF-1固态发酵产生淀粉酶的条件进行了优化。

Furthermore, we screened Jurkat cell lines which stably expresses sense and antisense IL-2Rαgene via neomycin selection.

同时，利用构建的质粒具有Neo抗性，用含G418的甲基纤维素半固体培养基进行了稳定整合反义IL-2Rα基因细胞株的筛选工作。

A genome library of Serratia marcescens CW-W-90-3 strain was established by "shot gun" method and screened for phospholipase activity on LB-egg yolk plate.

通过鸟枪法构建了粘质沙雷氏菌Serratia marcexcens CW-W-90-3菌株的基因组DNA文库。

In the present study, three normal bras, aerobic spore-bearing bacilli, yeast fungus and streptobacterium plantarum, were isolated, identified and screened from intestinal tract of healthy rabbits. After safety and drug sensitivity test, a compound microecomodulator was produced by combining the three floras, each was cultivated separately, and applied to weanling immature rabbits. The effects of the microecomodulator were evaluated by monitoring animal body weight, food consumption and diarrhea incidence rate and recovery rate of the rabbits with diarrhea.

本实验从健康家兔肠道正常菌群中经分离、鉴定，筛选出3种有益菌种：需氧芽孢杆菌、酵母菌和厌氧乳酸杆菌，经安全试验和药物敏感试验合格后，分别发酵培养，制成复合型微生物制剂再应用到断奶幼兔中，观察对幼兔体重、消耗饲料量、腹泻发生率等的影响，同时应用微生物制剂治疗腹泻兔，观察其治疗效果。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

Methods 34933 serum samples were screened by ELISA method on Treponema pallidum antibody. The samples with probable positive result were retested by toluidine red unheated serum test and Treponema pallidum particle assay. Results There were 559 serum samples with probable positive result.

对34933例标本采用酶联免疫吸附试验进行梅毒螺旋体的初筛，初筛结果为可疑阳性的样本进行梅毒螺旋体明胶凝集试验和甲苯胺红不加热血清反应素试验的检测。

One L-isoleucine high-producing strain SW0370 was screened through protoplast fusion, shake flask compound screening, genetic marker test and single clone isolation and continuous passage cultivation.

3确定了亲株ISW330和AS1.495原生质体形成和再生及进行原生质体融合的最佳条件，采用原生质体融合技术最终筛选出一株带有目的遗传标记的L-异亮氨酸高产菌SW0370〓。

2The protoplast formation and regeneration and fusion conditions of strain ISW330 and AS 1.495 were studied in this thesis. One L-isoleucine high-producing strain SW0370 was screened through protoplast fusion, shake flask screening, genetic marker test and single clone isolation and continuous cultivation for generations.

2确定了亲株ISW330和AS1.495原生质体形成和再生及进行原生质体融合的最佳条件，采用原生质体融合技术最终筛选出一株带有目的遗传标记的L-异亮氨酸高产菌SW0370(Leu~-+Met~-+AEC~r+á-AB~r)。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。