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The recombinant pIND-ndr2vector and pIND vector were tranfected into GLC-82cells respectively,and the cell clones were screened with G418and zeocin.

连有ndr2基因的载体（pIND-ndr2）用脂质体方法转染到培养的肺腺癌细胞GLC-82中。

We screened a set of Lewis base organocatalysts derived from chiral pipecolinic acid and tert-butanesulfinamide for the reduction of-N-Cbz-1- phenylpropenamine, including newly designed ones and some of those previously developed in our lab. However, only moderate stereoselectivities, albeit high yields were obtained.

在本课题组前期研究的基础上，我们筛选并设计了一系列以手性哌啶酸和叔丁基亚磺酰胺为母体的有机小分子路易斯碱催化剂，它们能催化三氯氢硅对-N-苄氧羰基-1-苯基丙烯胺的不对称还原，获得很高的收率和中等的对映选择性，并且具有很好的底物普适性。

The optimal boron concentration for growth of pea (Pisum sativum L.) andcucumber (Cucumis Sativus L.) plants was screened by designing a series of boronconcentration in nutrient solution and the influeuce of boron deficiency or toxicityon integrity of cell membrane in different organs of the two plants was studied bymeasuring pH of the nutrient solution,〓 concentration and conductance in rootexudate solution and extracting solution of different organs from the two plants.

用不同浓度硼的营养液培养豌豆、黄瓜植株，筛选和确定在温室条件下生长所需的最适硼浓度和硼毒害浓度；进而通过对缺硼、对照和硼毒害处理后营养液pH变化，根分泌物和不同组织浸提液中K+浓度和电导率的测定，研究了缺硼和硼毒害对两种植物不同组织细胞膜完整性的影响。

According to the results of agronomic traits and isoenzyme analysis, 20Gywas screened as the suitable dose for two varieties of Polianthes mutagenic L.

经农艺性状及同工酶分析，初步筛选出2个晚香玉品种的适宜辐照剂量为20Gy。

The recombinant Hansenula polymorpha strains for secretory expression of HBsAg were screened and cultured in YPD, BMMY and MM media respectively. The expression levels of HBsAg were determined by ELISA, and the virus-like particles were observed by transmission electron microscopy.

将其电转化多型汉逊酵母尿嘧啶缺陷型宿主菌ATCC34438(Ura3-)，筛选分泌表达HBsAg的汉逊酵母工程菌株，并比较在YPD、BMMY、MM3种培养基中分泌表达HBsAg的水平，ELISA检测培养上清液中HBsAg的表达量，透射电镜观察类病毒颗粒。

Results Multicopy recombinant plasmids pDGXHP2.0-2M Fα-HBsAg and pDGXHP2.0-4MFα-HBsAg were constructed. Three recombinant Hansenula polymorpha strains for secretory expression of HBsAg were screened.

结果 构建了HBsAg汉逊酵母多拷贝表达质粒pDGXHP2.0-2MFα-HBsAg和pDGXHP2.0-4MFα-HBsAg，筛选获得了分泌表达HBsAg的汉逊酵母HP/2MFα-HBsAg和HP/4MFα-HBsAg。

Polyvinylidene fluoride was used to prepare solvent base fluorocarbon coating, and the solvent constitution of PVDF coating was screened and optimized.

摘要用聚偏氟乙烯树脂制备了溶剂型氟破涂料，筛选并优化了PVDF涂料的溶剂组成。

The results Showed that the highest transformation efficiency was obtained through the following transformation procedure: after preculture for 2 d, the explants were in fected 3 min with 50 mg/L acetosyringone and OD600=0.5 Agrobacterium culture liquid, and then co-cultivated for 2 d. Resistant shoots from leaf disks were screened on MS0.4 mg/L NAA + 100 mg/L Cef medium supplemented with 10 mg/L Km.

结果表明：2 d的预培养，OD600值约为0.5的农杆菌菌液添加50 mg/L AS，侵染时间3 min，2 d的共培养，从分化培养基获得的抗性芽在生根培养基MS+0.4 mg/L NAA+10 mg/L Km+100 mg/L Cef上进行延迟筛选，有利于获得较高的转化效率。

Methods Forty - six prelingual deaf children and 30 children with normal comprehension were obtained, and the del（ GJB6 - D13S1830） in the Cx30 gene were screened by polymerase chain reaction in 2 groups.

方法收集46例散发的语前聋患儿及听力正常健康对照30例血标本，提取DNA后利用聚合酶链反应分析方法，筛查Cx30基因del（GJB6-D13S1830）突变。

The friable embryogenic callus with small grains and without proembryo formation was screened in the process of continuous subcultures for 4~6 times on the medium with low content of sucrose from embryogenic cultures induced from immature embryos in litchi (Litchi chinensis Soon.). The well-scattered embryogenic cell suspensions could be rapidly established from the friable embryogenic callus in 2 liquid initial MS media respectively supplemented with 2,4-D 2 mg/L or 2,4-D 2 mg/L, KT1 mg/L and AgNO3 5 mg/L shaking at 100~120 r/min within 10-14 days.

荔枝幼胚诱导的胚性培养物在低糖条件下连续继代4~6次左右，可筛选到颗粒细小、不含原胚的松散型胚性愈伤组织；以这种松散的胚性愈伤组织作为起始材料，在附加2，4-D 2mg/L或2，4-D 2mg/L、KT1 mg/L、AgNO3 5mg/L的MS液体启动培养基上振荡培养（100~120 r/min）10~14 d，即可建立起分散性良好的胚性悬浮细胞系。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。