查询词典 screened

A research method has been screened preliminarily for the active position of analgesic effect of the IPM.Key words:Indigofera Pseudotinctoria Matsum; Extraction parts; Analgesia; Effect position

马棘对实验小鼠具有明显的镇痛疗效；初步筛选了马棘镇痛作用有效部位的研究方法。

A new eucaryotic expression system of recombinant PoIFN-γ was also constructed so that the denaturalization and refolding process is avoided. The target gene coding PoIFN-γ2 mature protein was subcloned into expression vector, transformed into Pichia pastoris, The His〓 Mut〓 phenotype transformants were screened, fermented in flasks and induced by 1％methanol. The expressed product in culture solution and sedimentation have antiviral activity on VSV and immunological activity proved by indirected immunofluorescence antibody and Dot blotting assay.

为了构建可对表达产物修饰、加工的真核表达系统，使表达产物具有自然干扰素的活性，无需变性、复性，我们将PoIFN-γ2成熟蛋白基因连接酵母整合表达载体，电转化毕赤酵母，筛选了His〓Mut〓表型转化子，摇瓶培养，1％甲醇诱导表达，培养上清和菌体裂解上清均有抗VSV病毒活性，经间接免疫荧光抗体检测和Dot blotting鉴定，重组酵母菌诱导表达产物具有免疫活性。

Methods We electronically searched The Cochrane Library (Issue 4, 2009), PubMed (1980 to 2009), Ovid MEDLINE (1950 to 2009), Ovid EBM Database (1991 to 2009), EMbase (1980 to 2009), CBM (1978 to 2009) and CNKI (1979 to 2009) to collect literature about intravertebral analgesia for extern al cephalic version. We screened randomized cont rolled trials according to the predefined inclusion and exclusion criteria, extracted data and evaluated the quality of the included studies, and then performed meta-analyses by using RevMan 5.0.13 software.

计算机检索Cochrane Library （2009年第4期）、PubMed (1980~2009)、Ovid MEDLINE (1950~2009)、Ovid循证医学数据库(1991~2009)、EMbase (1980~2009)、CBM (1978~2009)和CNKI (1979~2009)，按纳入和排除标准筛选椎管内镇痛在外倒转胎位术中应用的随机对照试验，提取资料及评价文献质量后，采用RevMan5.0.13软件对数据进行Meta分析。

Several anti-tumor cell lines and carbonic anhydrase Ⅱ model were established. More than 10000 compounds and extracts from plants were screened using these models and about 10 compounds showed strong inhibitory activities. Two species of plants Inula helianthus-aquatica and Saussurea deltoidea were chosen to investigate chemical and bioactivity constituents based on the results of screening of extracts and fractions. 58 compounds were isolated and identified from these two plants, 6 compounds were found to be new and 14 compounds showed anti-tumor activities. We also studied the anti-tumor mechanism of the compound bigelovin isolated from Inula helianthus-aquatica. Bigelovin was found with apoptotic activity against cell line U266 by lowering the amount of important transcription factor E2F-1 which inhibits the cell cycle at G0/G1 stage. Docking study on 7 natural new phenolic inhibitors of CA Ⅱ and 3D-QSAR study on 61 sulfonamide inhibitors of CA Ⅱwere also investigated. The calculated results were in good correlation with experimental results.

基于以上两种重要疾病和研究组方向，本论文工作开展了多个肿瘤细胞株和碳酸酐酶Ⅱ靶标模型的建立和筛选工作，累计筛选1万多个/次样品，发现10余个活性较强化合物；从粗提取物筛选结果中选取两种抗肿瘤活性强的菊科植物水朝阳旋覆花(Inula helianthus-aquatica)和三角叶凤毛菊进行了系统化学和活性成分研究，分离鉴定了58个化合物，发现6个新化合物和14个抗肿瘤活性化合物；通过合作对水朝阳旋覆花中活性较强的bigelovin进行了抗肿瘤作用机制研究，发现它可能是通过降低G2期重要的转录因子E2F-1，致使细胞周期阻滞在G0/G1期，引起多发性骨髓瘤U266细胞凋亡；开展了7个新的天然酚类CAⅡ抑制剂的对接研究和61个合成的系列磺酰胺类CAⅡ抑制剂的3D-QSAR分析，结果表明计算与实验一致。

STUDY SELECTION: After the preliminary searoh of the data, articles including treatment group and control group were selected, and the obvious irrelated studies were screened, and the full-texts of the rest articles were looked up.

资料选择：对资料进行初审，选择包括和处理组和对照组的文献，筛除明显不符合研究目的文献，对剩余的文献开始查找全文。

ISSR analysis of 11 main cultivated and spread varieties in Juglans Silillata Dode in Yunnan was conducted with new-born leaf as material, based on an optimal ISSR reaction system established and 8 primers screened.

以核桃当年萌发的新叶为材料，在建立了良好的反应体系基础上，筛选出8条引物对云南省11个主要栽培和推广的核桃品种进行了ISSR分子标记研究。

to enroll 30 NIDDM patients and 30 normal controls with similar demographic characteristics.Patients with hypoglycemia or ketonic acidosis were screened out.Some diseases,which might influence cognitive functions,were excluded,such as cerebrovascular diseases,endocrinoloogical diseases,alcohol and drug abuse,dementia and psychosis.

40至60岁，性别、文化程度、职业相匹配的NIDDM患者和对照组各30名，排除可能影响认知功能的各种神经和精神疾病、内分泌疾病、药物和酒精滥用者，糖尿病人需排除曾有严重低血糖发作和酮症酸中毒者。

to enroll 30 NIDDM patients and 30 normal controls with similar demographic characteristics.Patients with hypoglycemia or ketonic acidosis were screened out.Some diseases,which might influence cognitive functions,were excluded,such as cerebrovascular diseases,endocrinoloogical diseases,alcohol and drug abuse,dementia and psychosis.

40至60岁，性别、文化程度、职业相匹配的NIDDM患者和对照组各30名，排除可能影响认知功能的各种过敏神经和精神疾病骨折、妇科内分泌疾病骨折、情绪药物和酒精滥用者，糖尿运动病人需排除曾有严重低血糖发作和酮症酸中毒者。

Results: Twenty one differential protein spots were screened and 5 differential proteins were identified. They included Ezrin protein, transcription elongation factor A, and lamin A/C which were up-regulated in LoVo cells and non-metastatic protein expressed in isoform a (NM23 A) and heat shock protein 8 (HSPA8) which were up-regulated in SW480 cells.

结果：筛选出21个差异蛋白质点，成功鉴定出5种差异蛋白质，其中在LoVo细胞株中表达上调的蛋白有埃兹蛋白、转录延长因子A (transcription elongation factor A)和甘氨酸A/C，在SW480细胞株中表达上调的蛋白有NM23蛋白[non-metastatic cell 1 protein (NM23A) expressed in isoform a]和（heat shockprotein 8，热休克蛋白8）。

Two strains that are resistant to nisin with high levels (up to 2000IU/ml in MRS broth ) were screened from 362 strains. One strain Ls-5105 isolated from pickles was systematically identified as leuconostoc mesenteroides subsp. mesenteroides on the basis of morphology, physiological and biochemical features with modern analytical methods and classical methods.

经过分离筛选，从362株菌中得到两株能够耐受2000IU／ml乳链菌肽的高抗菌株，利用现代分析技术与经典分类法对其中一株菌Ls-5105进行系统细菌学鉴定，鉴定该菌株为明串珠菌属肠膜明串珠菌肠膜亚种Leuconostoc mesenteroides subsp。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。