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The invention clones geranyl pyrophosphate synthase GGPPS gene from the ginkgo to construct plant expression vectors which contain ggpps gene, the ggpps gene is inducted into immature embryos of the ginkgo to induct out the callus tissue by the mediating of agrobacterium tumefaciens, PCR and semiquantitative RT-PCR detect the conformation and expression status of exogenous target gene ggpps, high-performance liquid chromatography and an evaporative light-scattering detector are employed to determine the terpene lactones content inside the callus tissue of the ginkgo, and the obtained callus tissue of the ginkgo of which the terpene lactones content is increased is screened.

本发明从银杏中克隆香叶基香叶基焦磷酸合成酶GGPPS基因，构建含ggpps基因的植物表达载体，用根癌农杆菌介导，将ggpps基因导入银杏幼胚并诱导出愈伤组织，PCR和半定量RT-PCR检测外源目的基因ggpps的整合和表达情况，高效液相色谱法及蒸发光散射检测器测定银杏愈伤组织中萜内酯含量，筛选获得银杏萜内酯含量提高的转基因银杏愈伤组织。

Methods:By means of germinative test and agar diffusion,130 sorts of Chinese herbal medicine were screened synchronously.

采用琼脂扩散法和芽管试验法对 1 30种中草药进行同步筛选。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

MSCs were cultured in a conditional medium in subculture including: dexamethasone, vitamin C, and β-glycerophosphate. At the same time, we constructed the eukaryotic expression vector containing VEGF165gene. VEGF165 gene was transfected into MSCs by means of LipofectAMINE?2000. The stably gene expressive cells were screened with G-418 for 14 days.

在脂质体介导下将构建成功的真核表达载体pcDNA3.1- VEGF165转染到诱导培养的MSCs，G-418筛选阳性细胞克隆，将阳性克隆细胞和未转染的MSCs共同接种于β-磷酸三钙上，体外培养7天后，植入原大鼠肌肉内。

Lower temperature,lower effecitve alkali charge and shorter time resulted in a pulp with lower HexA content and higher screened yield.

较低的蒸煮温度、较低的有效碱用量和较短的保温时间有利于降低纸浆中己烯糖醛酸的含量，且纸浆的细浆得率也较高。

Protein moleculars interacted with Plk1 were screened with yeast two hybridation system, Plk1 cDNA was amplified from total RNA of murine embryonic stem cells by RT-PCR.

我们选择了实验室现有的小鼠胚胎干细胞cDNA文库作为基础，首先利用RT-PCR方法获得了小鼠Plk1基因。

The nl shell energies of high ionization ions have been calculated by using the improved screened hydrogenic ionization model.

采用改进的屏蔽氢离子模型计算高剥离态离子的nl壳层能量，并与实验值及其他理论值进行了比较。

The frequency-dependent opacities and the Rosseland mean opacity for mixture of inertia elements Ar, Kr, Xe are studied using the splitting screened hydrogenic model.

利用分裂的屏蔽氢不透明度模型计算了Ar、Kr、Xe惰性元素混合物随光子能量变化的不透明度以及Rosseland平均不透明度。

Mutant libraries created by saturation mutagenesis of each single amino acid site D168, A225, K434 and E435 were screened to further improve the capability of cytochrome P450 BM-3(A74G/F87V/L188Q) mutant from Bacillus Megaterium which can hydroxylate indole into indigo. Results showed that D168, K434 and E435 are located at the functional domain of P450 BM-3 protein while A225 is sited at the unfunctional domain.

为了进一步获得具有更高活力的细胞色素P450 BM-3(F87V/A74G/L188Q)突变酶，利用饱和突变技术对该突变酶的4个氨基酸位点D168、A225、K434、E435分别进行单一的随机突变，通过对其羟基化吲哚生成靛蓝的催化性能表征，发现P450 BM-3氨基酸残基的168、434和435位均位于蛋白功能区域，而225位则位于非功能区域。

The screened bacteria and antinomycete could cause the deformation of pathogen hyphal growth in different degree.

和草莓灰霉病菌Botrytis cinerea抑菌效果较好的菌株，其中细菌3株，放线菌4株，木霉菌1株。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。