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For one show, she screened half a dozen actors and finally settled on a 17-year-old Chinese student in the United States - He has a very mature voice totally belying his age.

&为了一个剧,她曾甄别6个演员,最后决定启用一名今年只有17岁的在美国的中国学生--&他有一个完全超越他年龄的很成熟的声音。

The major results obtained are: PCR-RFLP method is suitable for molecular identification of Shihu; 205 compounds were isolated from 10 species of Dendrobium, including 21 new compounds, and 28 compounds isolated from genus Dendrobium for the first time. Each of the extracts and compounds were screened for anticoagulant and antitumour activities separately. The results indicated that the ethyl acetate extract from Dendrobium chrysanthun has significant anticoagulant function; the antitumour function of the 4 bibenzyl components from Dendrobium chrysotoxum is stronger than that of phenanthrene, of which the erianin has significant anti-angiogenic effect both in vivo and in vitro; components with moderate or stronger antitumour activities were found in the extracts of D. thyrsiflorum, D. chrysanthun and D. trigonopus; quality standard for 5 species of Dendrobium was drawn up use active or indicative components as control.

重要研究结果有:经方法学研究,认为PCR-RFLP法适于作为石斛属药材分子标记鉴定方法;对10种石斛进行化学成分分离鉴定,共分得205个化合物,其中新化合物21个,首次从石斛属分得的28个;对提取物和各单体化合物分别作抗凝血、抗肿瘤等活性筛选,结果说明束花石斛乙酸乙酯部位有显著的抗凝血作用,鼓槌石斛的4个联苄类成分抗肿瘤作用强于菲类,联苄类毛兰素在体内外有显著抗血管生成活性,球花石斛、束花石斛及翅梗石斛均筛到中等或较强抗癌活性的成分;选择有活性或指标性成分作对照品,对5种石斛草拟了质量标准。

The mouse embryonic stem cells cDNA library was screened by yeast two-hybrid assay,some candidate molecules were get,including DDX39 DEAD (Asp-Glu-Ala-Asp box polypeptide 39, BAT1A(HLA-B-associated transcript 1A), NAC1 ( nucleus accumbens-1 ), BicD2 (bicaudal D homolog 2)、MLL3 (myeloid/lymphoid or mixed-lineage leukemia 3). NAC1 and MLL3 were associated with regulation of gene transcription. DDX39 andBAT1A were associated with pre-mRNA processing and mRNA export from nucleus to cytoplasm. BicD2 was associated with the regulation of Golgi body.

通过利用酵母双杂交方法,以小鼠全长Plk1作为诱饵蛋白筛选小鼠胚胎干细胞cDNA文库中与其相互作用的蛋白,获得了DDX39DEAD(Asp-Glu-Ala-Aspbox polypeptide 39、BAT1A(HLA-B-associated transcript 1A)、NAC1(nucleus accumbens-1)、BicD2(bicaudal D homolog 2)、MLL3(myeloid/lymphoid or mixed-lineage leukemia 3)等几个候选蛋白,其中NAC1、MLL3与基因的转录调控有关,DDX39、BAT1A与mRNA前体的剪切、加工及mRNA从细胞核转运到细胞质紧密相关,而BicD2与高尔基体的调控紧密相关,提示我们Plk1与基因转录、mRNA前体的剪切、加工、mRNA的运输及胞质分裂中高尔基体的调控有关。

Extracts with higher antioxidant abilities, including hot water and ethanol extracts of Boehmeria nivea leaves and roots, were screened and then added into soy bean oil to study effects on oil stabilities of oxidation during storage and also to develop new XO paste products containing Boehmeria nivea leaves and roots. The extents of oil oxidation in XO paste were evaluated by measuring POV and TBA values. Results indicated that after 8 day storage at 70℃, the POV values of soy bean oil with addition of 0.1% of ethanol extracts of leaf and root were 56.27± 0.12 meq/kg and 57.13± 0.50 meq/kg respectively, which were both greater than that of control samples(53.33 ±0.31 meq/kg), showing that extracts of leaf and root do not have the abilities to inhibit oxidation of soy bean oil.

第二部分将抗氧化能力较高的萃取物,包括苎麻叶与根的热水及乙醇萃取物添加於大豆油中,探讨其对油脂贮藏安定性之影响及开发加入苎麻叶或根的XO酱产品,并藉由测定油脂中过氧化价(peroxide value,POV)和硫巴比妥酸值(thiobar-bituric acid value,TBA)值评估其油脂氧化程度,结果贮存在70℃下第8天时,添加0.1 %苎麻叶与根乙醇萃取物,其POV值分别为56.27±0.12 meq/kg与57.13±0.50 meq/kg,皆较对照组(53.33 ±0.31 meq/kg)高,显示以0.1 %的苎麻叶和根萃取物添加大豆油中并不具抑制油脂氧化作用。

Objective To clone the Bombus ignitus venom protease gene screened from the expression sequence tags of the bumblebee, Bombus ignitus and express it in Spodoptera frugiperda Sf-9 cell.

目的 克隆表达序列标签中筛选出的红光熊蜂蜂毒蛋白酶基因,在草地夜蛾细胞Sf-9中进行表达。

Methods The BiVP gene was screened by the ESTs technology and cloned by PCR method from the cDNA library of the bumblebee, Bombus ignitus. It was reconstructed to form the baculovirus transfervector pBac1-BiVP and expressed in Sf-9 insect cell. The recombinant BiVP was purified by the fast protein liquid chromatography system.

利用ESTs从红光熊蜂cDNA文库中获得BiVP基因,以PCR技术扩增出BiVP的完整cDNA,并构建病毒表达载体PBac1-BiVP,通过昆虫杆状病毒表达系统,在Sf-9昆虫细胞中进行表达,表达产物用快速蛋白液相色谱系统进行纯化。

The study recruited 104 volunteers aged over 35 years old. 84 subjects without diseases of liver, kidney, heart, diabetes mellitus, stroke, mental disturbance gastro-intestine and pregnancy or breasting were screened by biochemical analyses.

本研究招募104位35岁以上的志愿受试者,依生化血液分析筛选出84位无肝、肾、心脏病、未控制之糖尿病者、怀孕或哺乳、最近一年中风者、精神方面疾病及肠胃道方面的疾病志愿者。

In this paper, an efficient and easy biological screening method based on the sensitivity to lovastatin of Neurospora crassa was built to deal with the mutant strain rudely by UV mutation. A high-level strain was screened in the shaking flask culture incorporating with TLC and HPLC. A comparison between this two methods also was originally discussed while identifying dyeing using bromophenol blue as the better treatment of TLC, and offering a kind of simple, practical, fast and economical determination qualitatively and quantitatively.

本文有鉴于此,通过紫外诱变处理,并基于粗糙脉孢酶对洛伐他汀的生物敏感性,对诱变菌株进行了初筛;同时,采用摇瓶培养,结合TLC和HPLC的分析方法,复筛出一株高产菌株;对复筛过程两种分析方法分别进行了初步探讨,比较其优劣,确定TLC法经溴酚兰显色剂显色处理为较佳方式;从而,建立了一套简单、实用、快速、经济且有效的洛伐他汀生产菌诱变筛选方法。

Methods Spontaneous aged dementia mice were screened out from 21 months old Kunming mice and then were divided randomly into 4 groups, namely control, westernmedicine, lower dosage Bugan Yangsui formula and higher dosage Bugan Yangsui formula group.

从 21 月龄昆明种小鼠筛选出自发老年性痴呆鼠,随机分为 4 组即空白对照组、西药对照组、补肝养髓小剂量组、补肝养髓大剂量组、另设老年学习记忆正常组。

Therefore, the identification and characterization of floral organ identity genes in Buxaceae is critical in elucidating the gene evolution related to floral organ formation. Floral organ identity gene homologues of Buxus microphylla ssp. sinica were screened, and nine gene homologues of B.

根据前人的谱系关系研究,黄杨科很接近真双子植物基群第二次主要复制事件的时间点,因此鉴定黄杨科植物的花部基因,将有助於厘清调控花部器官形成基因的演化情形。

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The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为:以确有疗效的中药复方为研究对象,以现代科学技术和传统中医药理论为技术支持,以该复方所治病证的药效响应为评价标准,以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。

I don't… don't know. He's unconscious.

我不……我不知道他休克了。