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The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Of the 5,732 patients screened, 5,452 (95%) provided an analyzable blood sample and 5,433 completed a questionnaire. Ethnic distribution was 75% white, 14% African American, and 4% Hispanic. Household income was at least $60,000 in 44.9% of subjects; 73.5% had some college or higher education; 80% were employed full- or part-time; and 68% were married or living with their partner.

进行筛检的5，732名患者中，5，452(95%)提供可检测的血液样本，且5，433人完成问卷调查表，种族的分配比例为75%白人、14%美籍非洲人和 4%西班牙裔，受试者的家庭收入44.9%至少60，000美元；73.5%受过高等教育；80%为全职或兼职；以及68%已婚或和他们的伴侣住在一起。

In the four placebo-controlled studies evaluating radiofrequency denervation outcomes in patients with "confirmed"l-z joint pain, the only study that screened patients with MBB demonstrated positive outcomes,157versus only one of three that used diagnostic intraarticularin jections.158–160 Ultrasound-guided medial branchand intraarticular blocks have also been demonstrated to provide comparable accuracy to fluoroscopically guided injections, although they may be less likely to detect low-volume intravascular uptake and are less accurate in obese patients.161–164 Because MBB are technically easier to perform than intraarticular injections and involve anesthetization of the nerves to be lesioned, it seems more logical to use these blocks as a prognostic tool before radiofrequency denervation.

在四个设有安慰剂对照的对于&确诊&小关节疼痛的患者行射频神经消融后的结果的研究中，唯一一个用MBB筛选研究对象的实验表现出阳性结果，而其他3个用关节内注射筛选的实验中只有1个表现出阳性。超声介导下的内侧支关节囊内阻滞被证实较透视介导更精确，虽然其血管内吸收剂量较低且在肥胖的患者中精确性较差。由于MBB较之关节内注射技术上更简单，且能对受损神经进行麻醉，利用这种阻滞作为神经射频消融前的诊断方法较为合理。

The most effective group of the herb medicine was screened out according to the response of the mices reproductive system, and then it was applied to the healthy canines which were in the stage of anoestrus.

并将这5个组方分别喂给5组共40只性成熟的健康的小白鼠，根据对小白鼠的饲喂试验结果，将其中促进小白鼠生殖系统发育效果最显著的一个方剂应用于处于间情期的健康母犬，用以研究中草药对母犬的催情效果。

Methods The genomic DNA from Ephedra glauca was randomly transferred in Saccharomyces cerevisiae and Hansenula anomala,respectively by nitrogen ion bombardment.The recombined yeasts were screened and the content of ephedrine in ecto-and endo-recombined yeasts was determined by the methods of bromothymol blue indicator selection,slant cultivation,liquid culture,copper chromic salt qualitative test,and RP-HPLC determination.

方法通过N 注入介导蓝麻黄基因组DNA分别在酿酒酵母Saccharomyces cerevisiae和异常汉逊酵母Hansenula anomala中随机转化，转化后的酵母菌经BTB指示性辅助筛选、斜面传代和液体培养后，用铜铬盐定性检识和RP-HPLC方法，检测重组酵母菌胞外和胞内麻黄碱的量。

SR-B, a colorless crystal antimicrobic compound, was extracted and purified from broth of marine actinomycete N331 isolated and screened from sea mud. Its molecular structure was characterized by MS and various NMR spectra.

从分离筛选的海洋放线菌N331的发酵液中分离、纯化得到一种无色结晶抗菌化合物SR-B，通过对其质谱、多种核磁共振谱的结构信息进行分析，鉴定其分子结构。

AF297709 , which has specific interaction with the promoter region of hTERT, was first screened out of the HeLa cells cDNA library with the method of yeast one-hybrid assary in 2000.It was found that TRAP has positive activation effect on the transcription starting of hTERT.

人端粒酶催化亚单位hTERT组分的水平决定了细胞中端粒酶的活性；hTERT表达是端粒酶激活的关键步骤，一些转录因子、肿瘤抑制因子、细胞周期抑制因子、细胞寿命决定因子、激素受体和病毒蛋白参与了hTERT表达调控。

Athematic model is based on the cooking experiments,it could predict pulp properties at a given cooking conditions,at the cooking conditions of effective alkali charge 17%,sulfidity 29.5%,temperature 150℃,time 135 min,the pulp properties predicted by model were:Kappa number 18.1(upper 21.5,lower 15.3),screened yield 50.3%(upper 51.2%,lower 49.5%),brightness 34.1%ISO(upper 36.1%ISO,lower 32.2%ISO) and viscosity 1429 mL/g(upper 1627 mL/g,lower 1232 mL/g).

ODDE可以根据蒸煮工艺条件预测蒸煮结果，当蒸煮条件为：有效碱用量17%，硫化度29.5%，蒸煮温度150℃，保温时间135 min时，MODDE预测的蒸煮结果为：卡伯值18.1(上下限分别为21.5和15.3)，细浆得率50.3%(上下限分别为51.2%和49.5%)，白度34.1%ISO(上下限分别为36.1%ISO和32.2%ISO)，黏度1429 mL/g(上下限分别为1627 mL/g和1232 mL/g)。

Two RAPD molecular markers located on different arms of 2Ai-2 chromosome from Thinopyrum intermedium were selected and the generations of the hybrid generation derived from Zhong 4 Awnless and wheat were analyzed. Two chromosome translocation lines were screened for wheat breeding program to control BYDV.

本研究采用RAPD方法，对具2Ai-2端体和完整染色体异附加或异代换系进行分析，从随机引物中，选出两个分别位于2Ai-2染色体两臂上的分子标记，然后用这两个标记对中间偃麦草和普通小麦的杂交后代进行筛选，选出小麦抗黄矮病易位系新材料。

Basidiomycetes in Dinghushan were screened for the antimicrobial activity, and their active secondary metabolites were investigate...

以鼎湖山的担子菌为材料进行了抗菌生物活性筛选和活性物质的研究。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。