查询词典 screened

I screened the film at Harvard University Divinity School, where we had a lot of clergy, rabbis, etc. I've also screened it with a group of clergy – a bunch of priests on a retreat.

i放映电影在哈佛大学神学校，我们在那里有很多神职人员，犹太教等，我也甄别，它与一组神职人员-一群神父就退却。

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

The results showed that the tested results by PCR and HPLC were identical, the 22 fine Oo strains screened from grape production area produces no histamine, tyramine, nor putrescine, therefore, from the point of view of biogenic amines, 22 fine Oo strains screened in China possesses fairly high safety in metabolically biogenic amines.

结果表明，PCR检测结果与HPLC测定结果一致，从我国葡萄产区筛选的优良酒酒球菌均不产生组胺、酪胺和腐胺，从生物胺角度，我国筛选的22株优良酒酒球菌具有较高的生物胺代谢安全性。

Permissible bending radius: not less than 6 times of overall diameter for unarmored cable ; not less than 12 times of overall diameter for armored or screened cable ; not less than 6 times of overall diameter for screened flexible cable .

推荐允许弯曲半径：无铠装层电缆应不小于电缆外径的 6 倍；有铠装层或屏蔽层结构的电缆应不小于电缆外径的 12 倍；有屏蔽结构的软电缆应不小于电缆外径的 6 倍。

Through three-step continual EMS mutation, the adenine autotrophy strain(Ml-3) and the adenine-requiring revertant strain(M2-8) were screened successively, and the resistant strain(M3-20) to ammonium ion variant was further screened.

将MY-1经连续3次EMS诱变，依次选育富含核黄素的腺嘌呤营养缺陷型菌株(M1-3)和腺嘌呤缺陷型的回复突变株(M2-8)。

Based on the achievements and developm ents in studying natural gas hydrates at home and abroad,the mechanism of causi ng the oil and gas reservoirs to be tightly sealed by the natural gas hydrates i s systematically analyzed in the paper through discussing the formation mechani sms,evolution properties and distribution laws of the natural gas hydrates.In li ght of the research on their control effects on petroleum accum ulation and reservoir preservation,several geologic models of oil and gas accumu lation and preservation related to the natural gas hydrates are proposed,such as vertically and laterally screened models,screened-by-drape and screened-by- contact models and syngenetically and epigenetically screened models,etc.

以国内外天然气水合物的研究成果和进展为基础，通过讨论天然气水合物的形成机制、演化特征以及其分布规律，系统分析了天然气水合物可以得天独厚地封盖油气藏的机理，探讨了其对油气藏尤其是气藏聚集和保存的控制作用，提出了天然气水合物聚集和保存油气的多类地质模式，如垂向遮挡和侧向遮挡、披覆遮挡和接触遮挡以及同生遮挡和后生遮挡等。

So in our test aantagonistic germ was isolated from ginger soil and was studied. 108 strains of germs were isolated by tube dilution assay from the soil of gingerin Qingmu town,Bazhong city. Q6 strain was screened from these strains of germsthough the first screened and rescreened double with deck agar method andimproved aperture-strikeing method.

本实验从巴中市青木镇堆放腐烂姜并且又长出健康姜苗的根际土中分离出106株细菌，在涂布有姜瘟病原菌青枯劳尔氏菌的肉汤培养基上进行初筛，得到6株对青枯劳尔氏菌有较强拮抗作用的细菌，通过摇瓶发酵后利用发酵液，采用改良的琼脂孔扩散实验复筛，获得一株具有强拮抗作用的菌株，命名为Q6号。

A test-potential method and a mean radius-screened charge loop iteration method are utilized to find the screened charges in the relativistic and the non-relativistic screened hydrogenic models.

利用试验势法和平均半径－屏蔽电荷迭代法研究基于解析势的相对论和非相对论屏蔽类氢模型。

The comparisons of electron ionization energies, ionic total energies and electron transition energies calculated respectively by More, Faussurier and the relativistic screened hydrogenic models, have been performed and the precisions of these physical quantities verify the preponderance of the relativistic screened model based on the analytical potentials.

与More和Faussurier屏蔽类氢模型比较，在电子电离能、离子总能量和电子跃迁能量的计算结果精度方面，基于解析势的相对论性屏蔽类氢模型占有优势。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。