查询词典 sars

Methods A nested RT-PCR was used for detecting one fragment of SARS-CoV RNA in oropharyngeal swabs from 3 SARS probable patients ,4 SARS suspect patients and other 27 patients with fever in Hangzhou, and the nested RT-PCR product from one SARS probable patient was sequenced.Meanwhile in these 3 SARS probable patients, other three RT-PCR methods, including a hemi -nested RT-PCR targeted for another fragment of SARS-CoV RNA, a real-time RT -PCR and a modified nested real-time RT-PCR, were employed to detect SARS-Co V RNA.Results Two positives were found in the 3 SARS pro bable patients, and none positive in 4 SARS suspect patients and other 27 patien ts with fever, using the nested RT-PCR.

方法对2003年杭州市3例严重急性呼吸综合征临床确诊患者、4例疑似和27名医学观察者的咽拭子标本用巢式实时RT-PCR检测SARS-CoV核酸，对阳性扩增产物进行核酸序列测定，同时对3例患者的标本应用半巢式RT-PCR检测另一位点SARS-CoV核酸片段，并应用常规实时RT-PCR技术及改良实时RT-PCR技术进行检测。

Methods: We analyzed nucleic acid sequence of the SARS-CoV and SARS-like CoV which were obtained from the GeneBank. The evolutionary trees of SARS-CoV and SARS-like CoV were made up by using the Brede virus as outgroup, and analyzed variation in single nucleotide sequence.

对GenBank登录的SARS冠状病毒和SARS样冠状病毒(SARS-like CoV)核酸序列进行了分析，以Breda病毒为外组，构建了SARS冠状病毒和SARS样冠状病毒系统进化树，并进行单核苷酸变异分析。

Infection control nurse had very high percentage of anxiety and the level of anxiety significantly influenced by religion devoutness, working experience, working nature, infection control license, SARS handling experience, and taking care of SARS patient and participate establishing the SARS Fast Response Center.

感控护理人员在SARS期间有高比例处於高焦虑状态，其焦虑程度会因个人基本属性（宗教信仰虔诚度、工作年资、工作性质、感控师执照、处理过SARS事件经验、收治过SARS病人及成立跨单位SARS工作小组或应变中心）不同而有显著差异。

3Clinical investigation: during the epidemicity of SARS, 21 SARS patients were admitted by Inner Mongolian Hospital between April and May , 2003. as a controlled group who were treated with anti-virus, antibiotic, immunization and allowance of nutrients: and another 21 SARS patients as clinical treatment group who were treated with both Erihemu-8 (3g once, twice a day, taken orally , ten days in all ) and the above medicines.

3临床观察：在SARS 暴发流行期间，对内蒙古自治区医院在2003年4～5月收治的SARS 住院患者选取21例作为临床对照组，给以抗病毒，预防感染，免疫调节，营养支持，对症；另选取21例SARS 住院病人作为治疗组，在上述治疗基础上加用蒙药额日赫木—8，3g/次，2次/日，冲服，疗程10天。

3Clinical investigation摘要: during the epidemicity of SARS, 21 SARS patients were admitted by Inner Mongolian Hospital between April and May , 2003. as a controlled group who were treated with anti-virus, antibiotic, immunization and allowance of nutrients摘要: and another 21 SARS patients as clinical treatment group who were treated with both Erihemu-8 (3g once, twice a day, taken orally , ten days in all ) and the above medicines.

3临床观察摘要：在SARS 暴发流行期间，对内蒙古自治区医院在2003年4～5月收治的SARS 住院患者选取21例作为临床对照组，给以抗病毒，预防感染，免疫调节，营养支持，对症治疗；另选取21例SARS 住院病人作为治疗组，在上述治疗基础上加用蒙药额日赫木—8，3g/次，2次/日，冲服，疗程10天。

The SARS virus origin which obviously differs from the theory of out space origin and the theory of wildlife origin was theoretically discussed based on principles of pollution evolution ecology,pathogenesis and infectant characteristics of SARS,and some relevant experimental evidence. It emphasizes on the fact that increasingly combined pollution in China provides a substantial basis for virus evolution.

针对当前广为流传的SARS病毒"太空起源说"和"野生动物来源说"两种理论，本文根据污染进化生态学原理和SARS病毒发病传染特征，以有关实验结果为科学依据，阐述了SARS病毒的起源问题，指出：我国当前环境日益严重的复合污染为SARS致病病毒的产生提供了适宜的外部生态条件和物质基础，SARS致病病毒可能起源于多个病毒的基因重组或融合，更可能是一种来自于感冒病毒进化而形成的超级感冒病毒。

The simulation outcome demonstrates that various control measures after April 20,2003 in Beijing and the public holidays starting from May lst to May 5th have a significant impact on containing the epidemic situation. The most dangerous days, around April 27, when the number of uncontrolled SARS patients in the society reached at its peak, can also be calculated. The SARS infectiousness number newly reported in Beijing has been at a low level since May 20. But simulation experiments describe that it may fluctuate with import of SARS infectiousness from outside Beijing, weakening of quarantine, more social activities, and so on.

同时应用动力学模型，对北京的SARS疫情进行分时间段的模拟与SARS实施控制因素影响分析，结果表明，2003—04—20以后一段时间内实行的各种控制措施，以及5月1日—5月5日的长假对于北京SARS疫情的控制是非常有效的；而4月27日左右北京市社会上未得到有效控制的SARS病人数达到高峰，是最危险的时期。5月20日以后，北京的SARS疫情基本保持在低发的平台期，但是如出现较多的外来SARS病例输入，而内部隔离强度又减弱、社交活动增加等情况下，北京SARS疫情可能会有一定程度的波动与反弹。

There are lots of uncertainties concerning the SARS epidemiological rules, pestiferous routes and the control methods till now. Therefore, it's important to understand the life cycle of SARS CoV since it could provide us some information on prevention of the SARS.

目前，人们对SARS CoV的了解、研究还远不够，对该病毒引起的SARS疾病的流行规律、具体传播途径、疾病的控制等还存在众多的疑问，本文的研究可为了解SARS CoV的生命规律，阻碍SARS CoV的传播，防治SARS提供依据。

Nucleoprotein is also a major immunogenetic antigen for SARS; however, several tests have shown that N protein had the reactivity with other coronavirus positive sera. It is therefore an advantage of S protein as recombinant target antigen for SARS serum diagnosis compared with recombinant N protein. Moreover, S protein has been shown to be the major antigenic determinant that induces neutralizing antibodies and protective immunity to SARS-CoV, so it is also important in designing SARS genetic engineering vaccine.

SARS-CoV S 蛋白由1255 个氨基酸组成，体外表达如此大的蛋白很困难，而且表达蛋白的可溶性不高；其次，实验已经证实，全长S 蛋白含有大量无关的表位，致使表达的全长S蛋白对SARS 阳性血清的检出率低于S 蛋白片段；另外作为疫苗研究，S 蛋白上大量无关表位作为异体蛋白进入体内，会引发机体的过度免疫反应，这也是SARS-CoV 可能的致病机理之一。

Reveal the characteristics and regular pattern of its epidemic.①Investigated the epidemiological history of 448 clinical confirmed SARS cases, drawing the map of coming places of primary cases and whereabouts of the secondary cases, clarify the reasons of SARS inputting and spreading.②Exploring transmission relationship between cases by drawing the chain-map and learning the main transmission process.③Selecting the chain-cases with the exact onset and contact time to calculating the average incubation of same generation cases on different transmission chains and the average incubation of different generation cases on same transmission chain, Calculating the incubation median, the fifth percentile and the ninety-fifth percentile of the incubation of the observed cases.④Analyzing the timeliness of admission and the treating condition through the statistical calculation of the onset time, the admission time, the hospitalization time as well as the diagnostic time of the suspect and clinical confirmed cases.

调查全省448例SARS临床确诊病例的流行病学史，绘制全省输入SARS原代病例的主要来源以及引起传播后被感染者的去向图，阐明SARS病例的输入与扩散原因；②用传播链图分析传播关系，了解SARS的主要传播过程；③选择SARS原代病例与续发病例接触日期、发病日期明确而完整的传播链病例计算不同传播链同一代之间以及同一传播链不同代之间的平均潜伏期，并计算观察病例潜伏期的中位数以及第5、第95百分位数，确定SARS的最短潜伏期和最长潜伏期；④对患者发病、就诊、住院时间以及医院诊断为SARS可疑病例、临床确诊病例的时间进行统计，分析SARS就诊的及时性以及临床诊治情况；⑤计算不同接触方式、关系密切程度传播SARS的危险度，筛选形成传播的主要危险因素；⑥检测不同人群和同一人群不同时间的SARS血清抗体，了解抗体的阳转时间和持续时间。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失