查询词典 sandwich

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

A Panini is a sandwich made from a small loaf of bread, typically a ciabatta.

不过很可惜的是它正在进行外部维修工程，并没有机会真正的造访它。

To develop a double antibody sandwich ELISA for quantitation of Cap43 protein in serum and initially study correlation between concents of Cap43 protein expression in serum from lung cancer patients and healthy adults and tumor histological pattern in lung cancer.

目的建立定量检测血清Cap43蛋白表达双抗体夹心ELISA 法，并初步探讨血清Cap43蛋白表达水平在肺癌患者与正常人群间有无差异，以及该蛋白表达水平的高低与肺癌组织学分型间的关系。

To develop a double antibody sandwich ELISA for quantitation of Cap43 protein in serum and initially study correlation between concents of Cap43 protein expression in serum from lung cancer patients and healthy adults and tumor histological pattern in lung cancer.

目的 建立定量检测血清CaP43蛋白表达双抗体夹心ELISA法，并初步探讨血清Cap43蛋白表达水平在肺癌患者与正常人群间有无差异，以及该蛋白表达水平的高低与肺癌组织学分型间的关系。

In recent years, boutique sandwich bars, offering such fillings as foie gras with onion confit, have spread across the business districts of Paris, attracting long lunchtime queues.

最近几年来，精品三明治快餐店已经发展遍布巴黎商业区，为食客提供红洋葱鹅肝酱，午餐时刻，吸引来大排长龙。

Light buffet included, 30 kinds of salad, 20 cold appetizers, 10 kinds of Japanese Sushi, 10 kinds of sandwich, 5 kinds of pizza, 2 kinds of soup, 5 kinds of bread and dessert, 3 kinds of fruit, 5 kinds of noodles, 5 kinds of Cantonese conjee, each person will be given a free Daily soup .

轻式自助餐出品：由三十种沙拉组成的沙拉吧，凉菜二十种，日式寿司十种，三明治十种，意大利比萨饼五种，汤两种，面包，甜品五种，水果三种，面五种，粥五种，每位赠送一份广东例汤。

Fourth of July cookouts were not the same, and BLT lovers complained that their favorite sandwich was impossibly dry.

7月4日仅仅厨师说不是和BLT爱好者抱怨说，他们最喜欢的三明治不能干燥。

Balloons, streamers, Turkish junk food, whirling dervishes, concerts, vendors shouting "buyurun" in every language necessary – as one sandwich vendor put it:"There's everything here. In every corner something is going on."（DAMARIS KREMIDA

气球、彩旗标语、土耳其垃圾食品、漫游的托钵僧、音乐会，摊贩们用每一种必要的语言高喊& buyurun &—就像一个三明治摊主说的：&这里什么都有，在每一个角落都有事情进行着。&

You know, everyone does want to be as free as a bird, but when we have to face the dilemma of being dinks or not, I suppose only few of us will be that determined. That's why the sandwich generation is still the mainstream of modern society.

电视业的发展，特别是有线电视的出现为美国人提供了更多的选择，随之也就出现了一种花很多时间看电视的电视迷，人们把他们叫做：couch potato。

Fish or cut bait; cold fish·第130讲:dinks; sandwich generation

学习英语目的不只在阅读，还有口语

We are in a real jam.

我们的麻烦大了。

Hey, it's Ahmet from India, that foreign exchange guy.

看，那是印度的阿曼特，国际交换生