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The proportions of Ⅰ~Ⅱgrade and Ⅲ grade were 73.8%、 83.1%; 45.7%、 65.2% between 92 samples of Urgur patients and 188 samples of han patients . Lymphy node metastasis in Uygur patients were greater than Han patients in Ⅰ~Ⅱgrade and Ⅲ grade (X~2=13.14, P.001; X~2=5.08, P.05).(3) The proportions of the expression of VEGF-AmRNA and its protein were 31.8%、 57.1%、64.3%, 27.3%、 53.6%、 59.5% in difference pathological grade in 92 samples, With increasing of the pathological grade,the VEGF-AmRNA and its protein expression are highly (X~2=6.26, P.05; X~2=6.21, P.05); The proportions of the positive expression of VEGF-AmRNA and its protein were 68.4

392例浸润性乳腺癌组织中VEGF-AmRNA和其蛋白的表达在不同组织学分级中其阳性率分别是31.8%、57.1%、64.3%，27.3%、53.6%、59.5%；二者在不同组织学分级中表达差异具有统计学意义(X~2=6.26，P.05；X~2=6.21，P.05)；在淋巴结转移组与非转移组中VEGF-AmRNA和其蛋白的表达阳性率的分别是68.4%、31.49，61.4%、31.4%，在淋巴结转移组VEGF-AmRNA和蛋白的明显高于淋巴结未转移组(X~2=11.96，P.001；X~2=7.79，P.05)；VEGF-AmRNA和其蛋白的表达

Two virus samples were collected from Malvastrum coromandelianum and these samples were tested by PCR. The results showed that a specific band about 500 bp was amplified from the samples Fz1 and Fs1 with degenerate primers PA and PB.

PCR产物测序进行序列比较发现：2组赛葵样本上病毒之间的差异极小，核苷酸序列同源性大约为96％，因此推测：这两组赛葵样本可能是由(来源：Af3BC7bab论文网www.abclunwen.com)同一种病毒侵染。

Detection results showed samples VA2, VA7 and VA8 from Malvastrum coromandelianum had weak reaction with MAb SCR18, and samples X2 from Siegesbeckia orientalis had weak reaction with MAb SCR18 and SCR106, while other samples had no reaction with MAbs.

用双生病毒兼并引物PA、PB对杂草样本进行特异性的PCR检测，结果发现仅有赛葵样本VA2、VA7、VA8和稀硷样本X2出现约500bp条带，说明这些杂草上存在双生病毒。

These suggest that the xenocrysts were captured by the enclave magma from the host magma. On diagram of total alkali vs. SiO〓(Middlemost, 1994), most samples of the host rocks plot within the area of diorite, the samples of the quenched margin plot within the area of gabbro-diorite and the samples of the enclave plot within the area of diorite.

在Middlemost(1944)提出的侵入岩全碱-SiO〓分类图解中，寄主岩的投影点落在花岗闪长岩范围内，淬冷边的投影点落在辉长闪长岩范围内，而包体的投影点大都落在闪长岩区。

Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出，等位基因平均值、观察杂合度、Fis值、Fit值皆较高，表明非洲菊等位基因较丰富，杂合基因偏多，且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium；PP8、na、Ⅰ、h、Gi及Fst皆表明，ISSR检测遗传多样性的能力最强，其次是RAPD，等位酶最低；根据遗传距离进行聚类，三种方法都把70个品种分成A、B、C、D、E五个大组，每一组中除少数品种发生位移外，大部份品种分类结果相似，且与形态分析结果有相似性，但在每一组中，品种间的聚类差别较大，表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同；Mantel检测表明，三种标记的分析结果有显著相关性，表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异；非洲菊为组培苗，三种标记的检测结果皆表明，混合样品可以作为个体样品的代表，对整个居群的遗传多样性进行评价；这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

The testing methods and targets were shown as below:Recorded milk weights from the 7th day postpartum to 15th day postpartum,and calculate the average day-milk yield;Milk samples were draw in 7th day and 14th day postpartum,and testing the milk fat,milk lactose,milk protein,milk Zn and the milk somatic cell counts;Blood samples were adopted at the 7th day postpartum,testing the levels of serum PRL; Blood samples adopted at the 1st day and the 12th day in oestrous cycle were used to test the levels of E_2 and P_4 in serum;In the experimental stage, registering the condition of parturition, the mean birth weight of calves,the first oestrus,the oestrus scycle postpartum and the conception rate of the first service after artificial insemination.

试验方法及所测指标如下：于产后第七天到第十四天记录日产奶量，最后计算每组平均日产奶量；每组随机抽取分娩后第七天、第十四天的奶样，测定乳糖、乳蛋白、乳脂、乳锌含量及乳体细胞数；于产后第七天，随机采取奶牛血样，测定血清中PRL含量；于发情周期的当天和第12天，随机采取血样，测定血清中雌二醇(Estradiol，E2)和孕酮(Progesterone，P)的浓度；记录奶牛的分娩情况、犊牛初生重、产后第一次发情日期、产后发情周期，并采用人工授精技术进行受精，记录第一情期受胎率，最后取平均值，作为衡量奶牛繁殖性能的指标。

Under optical microscope, the observational result indicates that the peach blossom stone samples from Gao Mountain contains transparent and distransparent minerals which forms pealike and red spots. The XRD testing result indicates that the mineral compositions in the samples are dickite, hematite, quartz and a little pyrophyllite. LRM testing result shows that the basic part of the samples is dickite, the red minerals with both well crystal shape and dip dye shape are hematite, and colourless and transparent mineral grains are quartz.

光学显微镜的观察结果表明，高山桃花石样品中存在透明和不透明矿物，其中不透明矿物形成了艳丽的红色斑点；XRD测试表明，样品的矿物组成为地开石、赤铁矿、石英以及少量的叶腊石；拉曼光谱测试结果表明，样品的基质部分为地开石，结晶形态完好的红色矿物颗粒与呈浸染状的红色部分均为赤铁矿，无色透明的矿物颗粒为石英。

Results (1) From more than 100 primers, 11 primers producing polymorphism and reproduceable bands were selected, 129 bands were amplified.(2) The percentage of polymorphic bands within different populations were 20.9%-55.0%.(3) The cluster map including all samples were obtained by UPGMA. In the map, there were six cluster groups and three individuals outside the groups. The branch with five samples of Zhongjiang Sichuan population were far from other samples in genetic distance.(4) According to the distribution provinces five groups were divided in genetic variance analysis. Genetic variance 80.44% existing within population, 8.29% among populations and 11.27% among groups.

结果 （1）从100多个引物中选择出11个多态性强和重复性好的引物，扩增得到129条带；（2）不同居群内多态位点比率为20.9%－55.0%；（3）用UPGMA法得到所有样本的聚类图，可分为6个主要分支组和3个组外个体，其中四川中江居群的5个样本聚为一组，并与其他样本遗传距离较大；（4）按产地分组，遗传差异的80.44%存在于居群内，8.29%来自于组内居群间，11.27%的遗传差异来自于组间。

EDS and Surface-profile results indicate that the stoichiometries of all the samples approach 2:3 and the thicknesses are over 500nm. XRD results indicate that the major diffraction peaks of the film match those of Bi2Te3.The film growth exhibits preferred orientation at (015) phase and many Bi, impurity peaks are observed. Hall and Seebeck coefficient measurement reveals that all the samples are n-type with the carrier density of 10^23cm^-3 and conductivity of 10^-5Sm^-1 which confirm the samples have nice thermoelectrically properties.

能谱分析和台阶仪测量结果显示本文所制备的薄膜Bi：Te原子比接近2：3，厚度都大于500nm；XRD测量结果显示薄膜的主要衍射峰与Bi2Te3标准衍射峰相同，在（015）晶面上择优选向明显，有少量的Bi、杂质峰存在；霍尔系数测试及Seebeck系数测量结果显示，薄膜都为n型半导体薄膜，载流子浓度量级为10^23cm^-3，电导率量级10^-5Sm^-1，具有良好的电学性能。

Transudate samples from genital duct of the syphilis patient and 100 positive serum samples were detected by PCR. There was a 100% coincidence rate between the detecting results of the transudate samples and the clinical results. The detecting results of serum were all negative.

PCR检测29例梅毒患者生殖道皮损渗出液和100例初筛阳性血清标本，生殖道皮损渗出液与临床诊断符合率为100%，血清检测结果均为阴性。

But this is impossible, as long as it is engaging in a market economy, there are risks in any operation.

但是，这是不可能的，只要是搞市场经济，是有风险的任何行动。

We're on the same wavelength.

我们是同道中人。