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PartⅡFeasibility of vitrifying normospermic samples using droplets without cryoprotectans Objective Discuss the feasibility of droplets to vitrify normospermic samples without cryoprotectants. Methods Normal prepared sperm were frozen by conventional freezing protocol and non-cryoprotectant freezing in droplets respectively. Freezing in droplets means freezing sperm in every 10ul drop. After thawing, compared the two groups'sperm mobilities.

第二部分微滴法无保护剂玻璃化冷冻正常精子的可行性探讨目的探讨在不添加冷冻保护剂的情况下微滴法玻璃化冷冻正常精子的可行性方法收集16份正常精液标本，上游处理后分别进行慢速冷冻和微滴法冷冻，微滴法即用拉细巴氏管将标本形成10ul的液滴，直接投入液氮内冻存，复苏后镜检其活动率。

The research showed that the quantity of the FC in the sea watter samples ranged ≤40～≥48 000 cfu/L; in the economic shellfish samples ranged ≤200～240 000cfu/100g.

报道了渤、黄海 6个重点沿岸海域(吕泗、青岛、北戴河、盘锦、大连和庄河)表层海水和几种经济贝类中的粪大肠菌群检测结果。

The process of finite element computing has to be repeated as many times as the number of samples in random response analysis of structure. Usually, the number of samples is very large even by adopting weightily sample technique, so the computing efficiency is slow.

结构的随机响应分析由于涉及到大量的样本，而使得有限元计算过程大量重复，尽管通过正交设计可减少分析样本数目，效率仍十分低下。

To address the quality problem of training samples, this article uses sample weightiness analysis to select training samples.

在国家科学数字图书馆中，学科主题词表中包含一些语义映射关系。

The mothod is simple, quick, acurate. In all of the samples, the contents of echinacoside is markedly more than that of acteoside, the content of the two active component in the wilding is higher than that in the planting. The content of sample in the different sizes gradually increase from the big to the small, and the contents of samples collected from the different hosts vary markedly. These results are useful for the quality evaluation of medicinal materials of C. tubulosa .

本法用于管花肉苁蓉的测定简便、快速、灵敏、重复性好，所测样品中有效成分松果菊苷比毛蕊花糖苷的含量均高，野生种的2种有效成分含量均比栽培种的高，将样品以个体长度分成大、中、小3种不同样品，所测结果表示其含量按大、中、小依次增加，不同寄主间的含量差异很大，为评价管花肉苁蓉的药材质量提供一定的依据。

The samples of 600℃ group didn't crystallize, but the color became darker and yellower as the peolongation of the heating time; the samples of 650℃ and 700℃ groups crystallized in different degrees, the translucency and Chroma decreased as the prolongation of the heating time, but the Value increased.

600℃组各样本均未晶化，随着保温时间的延长，其颜色逐渐加深，偏黄，650℃及700℃组各样本均发生不同程度的晶化，随晶化温度的升高及保温时间的延长，样本的透明度逐渐降低，亮度逐渐升高，色调变浅且饱和度逐渐降低

In this study,a simple method for preparation and characterization of three calcium zincate samples of different morphology was developed to improve the high-rate charge/discharge performance of zinc negative. The samples were synthesized,followed by direct reaction method and ball milling method.

为提高锌负极大倍率充放电的性能，先以直接反应法和球磨法合成了四边形、六边形和不规则三种形态的锌酸钙，并用X射线衍射光谱法和扫描电子显微镜法对其进行了表征，然后以恒电流充放电法考察了三者作为负极添加剂时对锌镍电池大倍率充放电性能的影响。

Methods: thirty two salivary acc tissue samples were divided according to the presence of perineural invasion or pain and 5 normal parotid glands and 3 acinic cell carcinoma tissue samples were taken as control. ngf was examined by immunohistochemistry analysis.

以32例acc标本为研究对象，患者按照有无嗜神经侵袭及疼痛症状进行分组；以5例正常腮腺及3例腺泡细胞癌作为对照，采用免疫组化法对ngf进行检测。

Methods With β-actin gene as a reference, reverse transcriptase polymerase chain reaction was conducted to dectect the expression of PSA mRNA in 35 BC samples, 12 paracarcinoma breast tissues and 10 breast adenofibroma samples.

以β actin作为内部参照半定量RT PCR方法检测 35例乳腺癌组织、12癌旁乳腺组织和 10例乳腺纤维瘤中PSAmRNA的表达。

We also for the first time compared the the storage stability of the allylamine plasma samples in the different mediums , including the hydrophilicity and biocompatibility, and the results indicated the samples had good stability in the different mediums.

同时还首次比较了丙烯胺等离子体修饰样品在多种不同介质中的储存稳定性，包括亲水性和生物相容性，结果显示样品在不同介质中均有较好的稳定性。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。