查询词典 sample method

Methods: Using proper sample treatment method, applying non-polar capillary column (HP-1) to replace packed column for the determination of sodium cyclamate in food.

采用适当的样品处理方法，应用弱极性的HP-1毛细管柱取代填充柱检测食品样品中的甜蜜素。

The method detection limits were calculated as 1.5 mg/kg for sodium cyclamate based on a 10 g sample,with the ratio of sign to noise more than 3 times.

但也有研究表明：甜蜜素具有致癌、致畸、损害肾功能等副作用[1，2]，很多发达国家早已全面禁止其在食品中使用。

Method］ With 3 kinds of representative local duckweed varieties selected from the each sample place in Yangzhou City and Kunshan City resp. as the tested materials and the anaerobic wastewater diluted at 5 times and 10 times from the cattle Farm as the tested sewage,10 groups of purification treatments matching with 5 different duckweeds on 2 concn. of sewage were set up to investigate the effect of different duckweed variety on the purification effect of N and P in sewage with different concn.

方法］ 从扬州市和昆山市各采样点选取的当地3种有代表性的浮萍品种为供试材料，以经稀释5倍、10倍的牛场厌氧废水为供试污水，设置5种不同浮萍对2种不同浓度污水的10组净化处理，在实验室条件下考察了不同浮萍品种对不同浓度污水氮和磷的净化效果。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

The geologists have attached much importance to the particularity of ReOs isotopic system and its application is getting popular. The author sums up and compares several kinds of sample digestion methods for ReOs isotope analysis (NiS fire assay, Carius tube digestion, high pressure asher, leaching, and water disposing), the procedures of separating Os ( conventional distillation, liquid Br2 and CCl4 or CHCl3 extraction, rapid extraction with low blank, minitype distillation) and purifying Os, improved mass spectrometry measurement of Os isotope and its application progress in the earth science research brought along the improved measuring method.

总结和对比了近年来ReOs同位素分析中样品分解的不同方法（NiS火试金法、Carius管溶样法、高压灰化消解法、浸提法、水样处理法）、Os的分离（常规蒸馏、液溴和CCl4提取、快速低本底提取、小型蒸馏）与纯化流程、Os同位素比值质谱测定法（NTIMS、ICPMS、微区原位分析）及随测定方法的改进ReOs同位素体系在地球科学研究中的应用进展。

This paper determined Li+ content of LiFePO4/C composite by air-acetylene flame atomic Absorption spectrum method,recovery rate of standard series sample with Fe2+ and PO3-4 and without Fe2+ and PO3-4 were determinated.

采用空气-乙炔火焰原子吸收法测定了LiFePO4/C复合物中的锂含量，考察了不加基体和加入基体的两种标准系列的回收率；因为目前还没有LiFePO4的标准样品和标准分析方法，所以实验采用模拟标准样品考察了不同酸介质和不同酸用量的影响，并进行了Fe2+、PO34-、Cr3+、Mg2+、Mn2+、Co2+、Ni2+离子对测定锂的干扰实验。

The method was to set up a freezing time ladder of the mixture of competented cell and plasmid along with a negative sample to research under the same condition of other aspects;The results revealed that the transforming efficiency was the highest at the freezing time of 20 minutes;Compared with the efficiency of other freezing times,the 20 minutes freezin...

在保证其他条件一致的情况下，设置大肠杆菌感受态细胞和质粒混合物的冰浴时间梯度并建立阴性对照进行研究。结果：冰浴20 min时转化效率最高。结论：与其他冰浴时间相比，冰浴20 min时的转化效率最高，实验重复性好，成本低廉，而且操作简便，值得推广使用。

This paper introduces a method for determination of polychlorinated biphenyls in the sample of marine sediments by microwave extraction and gas chromatography with electric capture detector.

建立了微波提取海洋沉积物样品中多氯联苯的气相色谱-电子捕获检测器的检测方法。

Through tests on the determination of interference from the sample color, not only is its interference eliminated but also the accuracy of the method improved.

通过对样品颜色干扰测定的试验，既消除了样品颜色的干扰，又提高了方法的准确度。

Through tests on the determination of interference from the sample color, not only is its interference eliminated but also the accuracy of the method improved.

培酸法适用于深色溶液分析，灵敏度和准确度较高，通常用于总钒的仲裁分析；但是该法只适用于测定总钒，四价钒与五价钒只能用其它方法测定，这样就会引入方法误差，使四价钒、五价钒与总钒不匹配。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。