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The patient avoided eating food rich of protein for three days and fasting for 12 hours before the blood samples were obtained at 7:00-8:00 in the morning, and had laid on bed for 15 minutes at least, 2ml of the blood samples were taken from median antebrachial vein, put into the heparinized tubles, then centrifugating by 1500/minute for 10 minutes,obtained the plasma from the blood, the plasma sample were kept in -72℃ refrigerator, ELISA method was used to determine the homcysteine level.

患者在标本采集前3天避免食用高蛋白饮食,空腹12小时以上,清晨7:00-8:00采血,采血时平卧位,休息15分钟以上,采集肘正中静脉血2ml,置于肝素抗凝管,以1500转/分离心10分钟后,分离血浆,用酶联免疫吸附法测定各管的HCY含量。

The experiment indicated,Using the anthrone color method observable knowledge sample sugar content is 8.8%,According to paper chromatography, Appraises its principal constituent using the anthracene for the glucose, the fructose, the mannose

实验表明,利用蒽酮比色法可测知样品的含糖量为8.8%,根据纸色谱法,鉴定出其主要成分为葡萄糖、果糖、甘露糖。

The reasons for significant error were studied by adopting TTC chromogenic method, analyzing alcohol extract of natural antimicrobic component's polarity and making sample appropriate diffusion before plate cultivated.

采用氯化三苯四氮唑对指示菌显色、分析天然产物乙醇浸膏中抗菌成分的极性、样品在平板培养前适当扩散等方法,对K-B法检测天然抗菌成分存在显著误差的原因进行了研究。

Uses method which the field investigation sample and the indoor analysis unify, 2007-2008 years live the unicast awnless brome lawn and the awnless brome and the alfalfa interlacing in the Inner Mongolian nationality university agriculture school test farm take 2 years mix broadcast the lawn and the colleague mix broadcast the lawn as the objects of study, have studied under the different sowing seeds way the forage grass to the soil physics and chemistry nature influence, finally indicated:the change tendency which under the different sowing seeds way increases the soil bulk specific gravity overall along with the soil layer depth to present after reduces first increases, is the 10-20cm soil layer is lowest; Looking from the 0-40cm soil layer's mean value, the unicast awnless brome lawn soil bulk specific gravity is smallest, the colleague mixes broadcasts the lawn soil bulk specific gravity to be biggest.

科尔沁草原是典型的农牧交错地区,长期以来,由于不合理利用草地资源造成该区生态环境严重恶化,农牧业生产受到严重影响[1]。建立高产优质的人工草地是解决我国高寒地区草畜供求季节不平衡的矛盾,促进草地畜牧业持续发展的关键措施之一[ 2]。无芒雀麦和紫花苜蓿都是营养价值高、适口性好、抗寒抗旱能力强的多年生牧草。将这两种牧草混播不仅能提高牧草产量和品质,延长草地使用年限,还能改善牧草的营养,提高牧草的适口性,有利于调制和青贮[3]。

In this paper, using bupleurum as an example, a method of obtaining chromatograms of volatile oil in crude drugs by using gas chromatography and converting the data to retention index and percentage area in order to get the fingerprint of each sample was presented.

本文以生药柴胡为例,介绍了以气相色谱法获得含挥发油生药的气相色谱数据,并换算成保留指数和归一化峰面积,得到每一个样品的指纹图,将未知样品与标准样品的指纹图相比较,从而鉴别未知样品的方法。

The results obtained by the proposed method in the bilirubin calibrator and control Sample were in good agreement with the target values.

为减少间接胆红素在DB测定中的反应,常规分析采用反应Indn后中止反应的方法。

Coli BL21(DE3) with the amount of 11.49% through thin layer chromatography scanning analysis. Cavia cobaya was immunized using the purified cytorrhyctes to produce the anti-serum. Through the determination of the optimum working conditions, the sensitivity test, the specificity test, repeatability test and sample simulation test, the indirect ELISA method was established, which is a broad-spectrum, rapid and specific to detect various of food-poisoning Vibrio simultaneously.

融合毒素蛋白包涵体经纯化,免疫豚鼠制备血清抗体,确定间接ELISA的最佳工作条件,并进行敏感性、特异性、重复性及模拟样品检测试验,建立了食物中毒性弧菌广谱、快速、特异的同步免疫学检测方法。

In order to distinguish them better, the method of RAPD was used at the same time to study 11 species of Cerambycidae, which belong to 7 genera, 2 subfamilies. By the experiment of filtration on 33 kinds of arbitrary primers, 6 arbitrary primers ( S4, S8, S22, S24, SI05 and S823 ) have been filtrated out, which can make the belts of electrophoresis to be revealed on DNA sample of majority longicorns in PCR reactions.

通过RAPD技术和酯酶同工酶电泳技术的研究,进一步证明了这两种天牛应归属为同一种的结论是正确的,并正式对光肩星天牛这个种下是否存在三个型的问题给出了肯定的答复。

The result makes clear, with anhydrous chloridize zinc, sulfur acting acetyl amine is raw material, it is OK to use chemical method the ZnS Na Mijing that preparation bead diameter controls in 10 Nm, earning sample is α- construction of mine of ZnS fine zinc, 6 brilliant are, form appearance is globose or close globose.

结果表明,以无水氯化锌、硫代乙酰胺为原料,采用声化学法可以制备粒径在10 nm左右的ZnS纳米晶,所得样品为α-ZnS纤锌矿结构,六方晶系,形貌为球形或近球形。

Results: The new method simplified sample pretreatment, improved chromogenic condition and easy to operate.

结果:新方法简化了样品处理,改善了显色条件,易于掌握应用。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。