查询词典 safranin

The rabbit model of osteoarthritis was established according to Hulth's method. The rabbits were sacrificed at the 8th week after administration of Yanghe Decoction for 14 days, and then rabbit tibia articular cartilage was removed. Sections of the cartilage were stained with Safranin O for histological examination. The cartilage histological characteristics were observed according to the method of Mankin. Immunohistochemical staining was performed to investigate the expression of VEGF.

按照Hulth法建立膝骨关节炎模型，术后第5周起至第7周末，阳和汤组予混有阳和汤的颗粒饲料，正常组及模型组均给予普通饲料；术后第8周分别取各组胫骨平台关节软骨，采用番红O染色，进行Mankin评分；免疫组织化学染色测定关节软骨中VEGF表达的阳性指数。

Disk height index of intervertebral disks on X-ray films, areas and average T2 relaxation time of high intensity zone of disks on T2-weight MRI and histological score on pathological sections （HE staining, Masson staining and safranin O-fast green staining） were measured at 2 months, 4 months and 6 months after operation.

术后2、4、6个月时对正常及模型椎间盘观察X线片上的椎间盘高度指数、MRIT2加权相上的椎间盘高信号区的面积和平均T2弛豫时间、椎间盘纤维环损伤区的形态学变化、组织病理切片（HE染色、Masson三色胶原染色和番红O-固绿染色）椎间盘组织形态学变化、生物化学变化和组织学评分等指标。

Actinobacteria retain the deep purple color of crystal violet; Gram negative bacteria (e.g. Cyanobacteria) are decolorized, and may be counterstained with other dyes, such as safranin, carbol fuchsin, neutral red.

革兰氏阳性菌会保持结晶紫的深紫红色；革兰氏阴性菌则会褪色，但可以被其他染料复染，如碱性藏红、品红或中性红。

3Chondrogenesis was assessed using Alcian blue staining, Toluidine bluestaining, Safranin O/Fast Green staining at 7 and 14 days, still collagenⅡimmunohistochemistry and aggrecan immunofluorescence at 4, 7 and14 days after initial chondrogenic induction of adipose -derivedmesenchymal stem cells.

3观察脂肪间充质干细胞在诱导后的生长增殖情况、形态变化，于诱导7、14天后应用阿尔新兰染色、甲苯胺兰染色、藩红0／固绿染色，以及4、7、14天后应用Ⅱ型胶原蛋白免疫细胞化学、aggrecan免疫荧光评价其成软骨能力。

The extracellular matrix wasstained positively for Alcian blue, Toluidine blue and Safranin O / FastGreen at 7 and 14 days after initial chondrogenic induction of adipose -derived mesenchymal stem cells, and indicated collagenⅡand aggrecanwere expressed positively.

2脂肪间充质干细胞在高密度的"微团"培养条件下，于诱导7、14天后阿尔新兰染色、甲苯胺兰染色、藩红0／固绿染色阳性，诱导4、7、14天后Ⅱ型胶原蛋白免疫细胞化学、aggrecan免疫荧光阳性表达。

Method］osteoarthritis condyles of femur (n=21) were collected from knee joint replacement and the paraffin sections were prepared after fixation and decalcification.after stained with safranin o/fast green, the shape structure of calcified cartilage zone was observed by microscope.

方法］标本均来源于临床诊断为膝关节退行性骨关节炎患者，共21例，其中，男1例，女20例。股骨髁负重区取材、修剪、固定后，常规制备石蜡切片，番红o／固绿染色，显微镜观察。［结果］本研究收集的标本平均年龄

Six weeks after implantation, small amount of oval lacunae appeared. PGA fibers began to be absorbed. The amount of collagen increased and Safranin O stained positively. Eight weeks after implantation, cartilage with single or occasionally double chondrocytes in round lacunae was found.

体内埋植4周后可见PGA纤维间被不成熟的软骨细胞充填。6周时出现椭圆形软骨陷窝，PGA纤维开始吸收，胶原增多，番红花—"O"有阳性染色。8周时软骨陷窝呈圆形，单个，双个对称或多个排列。

Staining results of ADSCs: The extracellular matrix Alcian blue staining, Safranin O/Fast Green staining and collagen Ⅱ immunohistochemistry were positive.

脂肪间充质干细胞诱导后的细胞化学染色结果：诱导后脂肪间充质干细胞胞外基质阿尔新兰染色、蕃红O/固绿染色、和Ⅱ型胶原免疫细胞化学着色阳性。

The materials were first fixed in different fixatives, embedded in paraffin, sectioned serially into 4fi thickness and stained with dyes of safranin and orange G, Heidenhain's iron haematoxylin combined with Mallory's connective tissue stain, buffered Magn-Lillie eosin methyl blue, Feulgen's reaction and methyl green-pyronin.

3凡有核仁外移的细胞，绝大部分为嫌色细胞，而在嗜色细胞中，尤其是在嗜酸性的细胞中极少见到这种现象，因此核仁外移和细胞的种类之间存在着一定的关系。

Results: Mast cells in uterus were mainly distributed in the myometrium, only a few in the endometrium. After ovum implantation ,the mast cells were comparatively less at the place of implantation. The number of mast cells of early and late pregnancy(1～5 dyas, 15～19 dyas) was apparently higher than that during middle pregnancy(P＜0. 05). Stained by alcian blue-safranin, both red and reddish blue mast cells decreased gradually in number during pregnancy, but the proportion of blue mast cells was increased. Critical electrolyte concentration of mast cells decreased as time went on.

结果：(1)肥大细胞主要分布于子宫肌层，子宫内膜较少，且胚泡植人部位的肥大细胞明显少于非植入部位；(2)妊娠早期(孕1～5d)和妊娠后期(孕15～19d)肥大细胞数显著多于妊娠中期(P＜0.05)；(3)Alcian蓝藏红组化染色后，可见妊娠过程中红色和红蓝混合色肥大细胞逐渐减少；蓝色肥大细胞所占比例逐渐增大，孕9～19d肥大细胞几乎全部为蓝色；(4)肥大细胞临界电解质浓度值随妊娠时间的推移而变小。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。