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The method is just like the QuikChange site-directed mutagenesis method except for its special primer. This pair of primers is composed of a long primer and a short primer which is central overlapped.Both of the Tm of 5\'sequence in long primer and Tm of 3\'sequence in short primer are above 66℃.The Tm of short primer is almost half of that of long primer.It is convenient to perform insertion or substitution mutation of DNA fragments larger than 20bp,In addition,it is highly applicable in research about the deletion mutation of DNA fragments larger than 2000bp.

我们实验室一直在寻找一种简单高效的可以用于基因的删除、插入及替代突变的方法，通过大量研究和实验发现，可以使用一种基于反向PCR原理，使用全新的引物设计的突变方法——&COP&突变法，该方法采用与QuikChange点突变法相同的操作步骤，其策略是设计一对中心重合的引物，长引物的5\'端序列和3\'端序列的Tm值均不低于66℃，短引物Tm值约为长引物一半，且与长引物中心重合互补。

DC power transmission simulationmodel is set up in this paper with the Matlab software, and various faults such as single-phasegrounding short circuit, phase to phase short circuit, three-phase circuit grounding shortcircuit and inverter bridge short circuit are simulated in this model.

用MATLAB软件建立了电力系统直流输电仿真模型，模拟了单相接地短路故障、相间短路故障、换流器桥臂以及单桥故障，经过近百次仿真运行，得出了各种故障下的特性曲线，准确描述了直流输电系统在发生大扰动后的暂态过程，并从理论上进行了科学分析，得出了各种故障对换相失败的影响。

The ABD is ready for analysis in 3 minutes with run-to-run cycle times of 5 minutes or less per sample.

ABD 在5分钟循环时间里面，分析每个样品只需要3分钟，或者更少的时间。

Bridge laying on both sides along the elevated track on the vertical run, lifting trolley along the bridge in laying the track on the horizontal run, a rectangular scope of work, you can take full advantage of the air bridge Below

桥架沿铺设在两侧高架上的轨道纵向运行，起重小车沿铺设在桥架上的轨道横向运行，构成矩形的工作范围，就可以充分利用桥架下面的空

We do not run the group like they run groups in the US where everyone has to stand up as if they were in Alcoholics Anonymous and confess how much money they have in the bank.

我们这个团体的运作方式与美国不同。在美国，每个人都必须像匿名戒酒会的成员那样站起来，交待自己在银行有多少存款。

Gates is run for foundation placed a series of regulations system, specific requirement foundation must not have proud " almsgiver psychology ", ensure run transparent and efficient.

盖茨为基金会运作定下了一系列的规章制度，明确要求基金会不得有傲慢的"施舍者心理"，确保运作透明而高效。

Listen, you want me to run Amman, let me run Amman, all right?

听着 你要我负责这次阿曼行动就让我放手做行吗？

Before using should though air load test run.before test run turn the amortise to the mimums. then expand it to avoid damage bcos of the impact of big area.

汽缸使用前应经空载试运转，运转前将缓冲调至最小，逐步扩大，以免产生过大冲击面而损伤汽缸。

The Appalachians run slightly from the northeast to southwest and the Rocky mountains run slightly from the northwest to southeast.

阿巴拉契亚山脉和落基山脉是美国的两座大山脉。（本细节有考"一句话简答题"的可能）6。

Apprize the stretch limousine service company as to the hire duration is to be for a smaller run or a long run to make sure you receive the correct stretch limousine .

满目豪华轿车服务公司以分期付款的期限是成为一个更小的来说还是长远来说，以确保您获得正确的舒展豪华轿车。

London based fashion photographer Matt Irwin might be is the next big thing in fashion photography.

总部位于伦敦的时装摄影师马特欧文可能会是下一件大事的时装摄影。

Based on IMC system,the response would be better if the model matched more satisfactorily.

由于系统基于内模控制设计，故模型匹配度越高，系统响应越好。