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KCR and HCD are erucic acid synthesis genes. In order to reveal the relationship between the synthesis of erusic acid and the quantitative expression of its functional genes, and to reveal the theoretic base of the breeding for high erucic acid content, the expression of KCR and HCD had been analyzed kinetically and quantitatively by using regular reverse transcription polymerase chain reaction and the internal standards of QuantumRNA18S during the growing period of high erucic acid content lines of Brassica Juncea.

研究应用常规RT-PCR(Reverse transcription polymerase chain reaction)的技术和QuantumRNA18S内标对芥菜型高芥酸油菜生育期内芥酸合成基因KCR、HCD基因的表达进行量化分析,了解芥酸的合成与功能基因量化表达的关系,为芥菜型油菜的高芥酸品种的选育提供理论依据。

Some sub-models of the original KIVA code were modified, by introducing into the Kelvin-Helmholtz and Rayleigh-Taylor model for spray breakup, the multiple laminar and turbulent characteristic timescale combustion model, the heat transfer model of Han and Reitz, the Hiroyasu and Nagle soot model and the improved Shell auto-ignition model were added to the original KIVA, to improve the predictions of engine spray, ignition, combustion, gas/wall heat transfer and emissions.

为提高模拟精度对标准的KIVA程序进行了改进,引入KH-RT雾化模型、层流和湍流多重特征时间尺度燃烧模型、Han和Reitz传热模型以及Hiroyasu和Nagle碳烟排放模型来模拟柴油机多次脉冲喷雾、燃烧、壁面传热及排放过程,同时增添改进的Shell自燃模型来模拟着火过程。

Methods Twenty-six NZW rabbits were randomly divided into three groups: a normal control group (fed on normal commercial rabbit diet, n =6), a hyperlipidemic group fed on high-fat ma total cholesterol (TC concentration at the beginning and the 12-week end point of the experiment determined,and all the experimental rabbits were sacrificed, and endothelial function test were performed on thoracic aortic rings prepared from the isolated aorta using the vascular endothelium-dependent relaxation parameter in response to acetylcholine The aortic atherosclerotic lesion expression of LOX-1 mRNA and protein were examined by RTPCR and Western blotting respectively.

方法新西兰大白兔随机分为正常对照组(普通饮食,6只),高脂饮食组(高脂饮食,10只)及普罗布考组(高脂饮食+普罗布考200mg/kg·d,10只)。实验开始前及第12周分别耳缘静脉采血测定血清总胆固醇。第12周处死动物,取胸主动脉,制备离体胸主动脉环对乙酰胆碱的反应以检查内皮功能,RT-PCR与免疫印迹检测凝集素样氧化低密度脂蛋白受体-1(LOX-1)基因与蛋白质表达水平。

By employing such a method, 5 series of elastomers, including natural rubbers, elastomeric polyester fibers and ethylene-octene, ethylene-methyl acrylate as well as SIS triblock copolymers, were investigated with the purpose of studying elongation induced phase structure and mobility changes of the samples. For NR samples, it was demonstrated that there exists a small amount of crystals at RT even for unelongated samples. For both NR and the soft segment of PEE, with the increase of elongation ratio, the elongation-induced crystallinity increases almost linearly in a certain region of R, in accord with the increase of the lamellar thickness of the crystals; The behavior of ethylene copolymers, in terms of elongation induced morphorpholgy changes, are quite different from NR and PEE. The crystals are found to be gradually destroyed with the increase of R, with accord with the decrease of the lamellar thickness; The motional behavior of the soft segment of SIS, changes from "liquid" to "solid", in terms of 13C NMR spin-lattice relaxation.For many of the aformented samples, the dependence of 13C spin-lattice relaxtion time T1 on R are different for different groups, indicating that the changes of mobility at MHz frequency region with elongation are different for different groups.

通过对天然橡胶、聚醚酯嵌段共聚物、乙烯-辛烯共聚物、乙烯-丙烯酸甲酯共聚物、SIS等高分子弹性体样品聚集态结构和分子运动的研究,获得了一些新的实验结果,如:给出了确切的实验证据,证明了室温下未拉伸的天然橡胶样品中存在着微量的结晶;发现随着拉伸比增加,天然橡胶及聚醚酯样品的软段部分会出现拉伸诱导的结晶,且结晶度在一定范围内呈现近似线性的增加,所形成晶片的厚度也随之逐渐增加;而乙烯共聚物随着拉伸比的增加,原有的结晶会被逐渐破坏,晶片厚度逐渐变薄,结晶中的缺陷逐渐增加;随着拉伸比的增加,SIS中PI软段部分的核磁共振弛豫行为会出现从液态到固态的转变等;在对上述多个体系的研究中,还发现不同基团的自旋-晶格弛豫时间随着拉伸比呈现不同的变化趋势,表明不同基团的高频运动随拉伸比增加有不同的变化规律。

The homology of nucleotides and amino-acid residue with the sequence in GenBank was 95.88% and 99.07%, 293 cells transfected with the recombinated plasmid express relucent green fluorescence.

转染293细胞后24-72h,可观察到明亮的绿色荧光,RT—PCR和Western blot分别从mRNA和蛋白水平上检测到frc基因在真核细胞中的表达。

Would you help me with the repo rt ?

你愿意帮我写报告吗?

It also features a Retransmit capability that allows for reset of the read pointer to its initial position when RT is pulsed low to allow for retransmission from the beginning of data.

它还具有一个重传的功能,允许对读指针复位到初始位置时,其脉冲逆转录是低到了转播允许从数据开始。

Reverberation Time is defined as the number of seconds it takes for the reverberant sound energy to die down to one millionth (or 60dB) of it's original value from the instant that the sound signal ceases.

混响时间( RT 是秒定义为多少能源需要的混响声音死亡下降到100万台(或60dB 的它)的的即时原值从声音停止信号。

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异;采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异;采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

Cobra venom factor was given to prevent hyperacute rejection and FK506 was given to prevent cellular rejection. The xenograft recipients were killed and cardiac xenografts harvested 4, 8, 12, 16, 24 hours after transplantation. Histologic examination was used to demostrate the degree of rejection and coagulation while revers transcriptase-polymerase chain reaction was used to detect the expression of tissue factor mRNA, and the guinea pigs hearts, harvested but not transplanted, were studied as controls in all samples.

建立豚鼠-大鼠异种异位心脏移植动物模型,使用CVF抑制超急性排异反应,FK506抑制细胞免疫排斥反应,分别在移植术后4、8、12、16、24h切取移植心脏,通过病理切片观察排异反应和凝血强度,同时通过RT-PCR方法检测组织因子mRNA表达强度,未移植豚鼠心脏作对照。

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And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47:5 法老对约瑟说,你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

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