查询词典 rt

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

It is believed that presupposition study within the framework of RT has some theoretic and practical significance.

在对全文内容加以总结的基础上进一步表明语用前提与关联理论相结合的研究方法的理论和现实意义。

Methods: the hbv transgenic mice were generated by microinjection of 1.3 copies hbv genome into the pronucleus of fvb/n zygotes. the pcr assay,elisa,rt-pcr and immunohistochemical methods were used to detect the hbv integration,replication and expression in the transgenic mice.

采用受精卵显微注射法，制备hbv转基因小鼠，用pcr，elisa，荧光定量pcr和免疫组化的方法研究hbv基因在转基因小鼠体内的整合、复制和表达情况。

Using the Hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which Sry transcription can be detected failed to yield any positive results of Xist. Female embryos at the pronucleus stage and 2-cell failed to produce any positive result of Sry and Xist too.

然后利用实验一确定的PCR条件，以Hprt为阳性对照，用巢式RT-PCR对小鼠早期胚胎进行Xist基因的转录分析，结果发现，转录Sry基因的睾丸组织以及雄性胚胎，从受精卵发育到囊胚的过程中，基本上不转录Xist基因；不转录Sry基因的雌性卵母细胞和雌性胚胎，从出现原核开始，到发育至2-细胞期的过程中，Xist基因一直不转录，但是，从4-细胞期开始，一直到孵化前囊胚阶段，雌性胚胎都转录Xist基因。

In order to clone the VIP gene in the gastrointestinal tract from beijing duck, one pair of specific primers to VIP gene was designed and synthesized according to the chick sequence (X80906). Encoding VIP cDNA fragments were amplified by RT-PCR from the total RNA in the Proventriculus, the Duodenum and the Jejunum of Beijing duck. Their PCR products were ligated into pGEM-T easy vector, which was transformed into E. coil JM109. Positive bacteria clones were screened and identified by PCR method and digested with the double restriction enzyme EcoRⅠ. The sequence of VIP gene fragment was also determined and analyzed.

为从北京鸭胃肠道中扩增血管活性肠肽基因，根据鸡VIP基因（GenBank登录号X80906），设计了一对简并引物，从北京鸭腺胃、十二指肠和空肠提取总RNA，通过反转录－聚合酶链反应扩增，将从腺胃、十二指肠和空肠中扩增出的产物克隆到pGEM-Teasy载体上，导入大肠杆菌JM109，阳性克隆经双酶切鉴定后测序，将测序结果与鸡和鹅（GenBank登录号为DQ023161）的VIP基因进行同源性比较。

The RT-PCR was sensitive and specific for HEV and did not amplify closely related bovine coronavirus and pseudorabies virus of pigs.

检测与HEV亲缘性较高的牛冠状病毒及猪的伪狂犬病毒均为阴性，最低可以检测到10个TCID50/100μl的病毒，说明该方法的有较好的特异性和敏感性。

The 6 AChE Genes from L.bostrychophila,L.entomophila and L.decolor were demarcated into Insect TypeⅠAChE Gene and Insect TypeⅡAChE Gene.4 Gene cloning of nAChR and mRNA expression level in L.bostrychophilaTwo nicotinic acetylcholine receptor subunit genes,Lb al and Lb a8,were cloned from the psocid L.bostrychophila.The full length cDNAs of Lb a1(GenBank Accession No.: EU871527) and Lb a8GenBank Accession No.

昆虫的两类AChE严格的区分，其中Ⅰ型AChE先与脊椎动物AChE聚合后才与Ⅱ型AChE相聚，说明两类AChE基因在物种分化前就已经分化。4嗜卷书虱烟碱型乙酰胆碱受体基因克隆及其mRNA表达水平研究利用RT-PCR和RACE技术成功克隆获得了嗜卷书虱2个烟碱型乙酰胆碱受体亚基的全长序列，分别命名为Lb a1(GenBank登录号：EU871527)和Lb a8(GenBank登录号：EU871526)。

It has been difficult to quantitate the amount of specific mRNA without an proper internal standard.

定量RT-PCR技术的关键之一是必须有适当的内参照标准。

RT-PCR was used to quantitate the relative abundance of ndrg2 mRNA expression and analyze the changes.

在小脑、延髓和海马等部位，ndrg2 mRNA的表达水平在16周胎脑中较高。

In addition, the decay rate of early sound energy varies with receiver positions.

同时，早期反射声能在不同位置上的衰减也不均匀，表现在EDT的偏差较RT的大。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。