查询词典 rt

In vitro transcripts from RNA5 wild type clone were co-inoculated into Tetragonia expansa with the RNAs of Hu3 isolate containing RNA1, 2 and 3. Following RT-PCR and Northern blotting results shows RNA5 could be biologically replicated in the leaves. The virus concentration of the isolate containing RNA5 in T. expanxa was higher than that of the isolate Hu3 evaluated by ELISA detection.

野生型RNA5的体外转录物与含Hu3(RNA1+2+3)分离物的总RNA混合接种番杏叶片的RT-PCR和Northern blot检测结果都表明，野生型RNA5能够在接种寄主体内复制，并且ELISA检测证明含有RNA5的番杏病叶中病毒浓度高于不含RNA5病叶的病毒浓度。

RT-PCR and SABC immunohistochemical technique were applied to detect the expression of Tiam 1 mRNA and protein in MO, ML and MH cells.

应用RT-PCR和SABC免疫组化技术分别检测Tiam 1 mRNA与蛋白在MO、ML、MH细胞中的表达。

This introductory chapter will explain what ticketing is and what RT is.

这个介绍章节将解释票务是什么和RT是什么。

Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

采集人体外周血标本15例，分成三份，分别作为新鲜血组、-80℃冻存血组和-20℃冻存血组标本，用TRI Reagent和TRI Reagent-BD两种不同的试剂提取RNA，以RT-PCR方法扩增β-Actin基因，经1.5%琼脂糖凝胶电泳，溴化乙锭染色，紫外透射仪下观察分析。

Design one primer pairs for EphB2 receptor and one primer pairs for GAPD as internal control. The RT-PCR was used to analyze the expression of EphB2 receptor tyrosine kinase in gastric cancer, colon cancer, lung cancer, liver cancer, kidney cancer, spleen cancer and abdominal wall cancer. The results were analyzed using Bio-RAD UV transilluminator with Quantity One software system.

采用RT-PCR方法，分别检测胃癌、结肠癌、肺癌、肝癌、肾癌、脾癌、腹壁癌7种肿瘤组织及相应邻近正常组织中EphB2受体的表达，应用Bio-RAD凝胶成像系统及在Quantity One软件辅助下分析测定表达结果。

We use 0.5% CMC to preserve the activity of Metarhizium anisopliae at room temperature. Then we carry on the RT-PCR semi-quantitative examination of the trehalase. The fresh Metarhizium anisopliae not preserved do control.

对用0.5%的羧甲基纤维素钠室温保存五个周的绿僵菌，进行海藻糖的半定量RT-PCR检测，用没有经过处理的固体培养基上培养14天的绿僵菌做对照。

We use 0.5% CMC to preserve the activity of Metarhizium anisopliae at room temperature. Then we carry on the RT-PCR semi-quantitative examination of the trehalase. The fresh Metarhizium anisopliae not preserved do control.

对用0.5%的羧甲基纤维素钠室温保存五个周的绿僵菌，进行海藻糖酶的半定量RT-PCR检测，用没有经过处理的固体培养基上培养14天的绿僵菌做对照。

In our research, the neutral trehalase gene in M. anisopliae was successfully cloned by PCR, RT-PCR, RACE and panhandle PCR, and submitted to GenBank, accession number are AY557613 and AY557612.

本研究利用PCR、RT-PCR、RACE、panhandle PCR 等方法成功地克隆了金龟子绿僵菌CQMa102 中性海藻糖酶基因，并登录NCBI 的GenBank，登录号为：AY557613， AY557612。

To elucidate the possible mechanism of differentiation and /or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor, the level of acetylated histone H3 was detected by Western blot, p21~ WAF1expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin，TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制，利用Westernblot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21WAF1表达量的改变。

To elucidate the possible mechanism of differentiation and/or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor , the level of acetylated histone H3 was detected by Western blot, p21(superscript WAF1) expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin， TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制，利用Western blot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21(上标 WAF1)表达量的改变。

Hanna: That's over now, isn't it?

都结束了，对吗

You must be ill. You look so pale.

你一定是病了，你的脸色苍白。