查询词典 rt

Rapid and reliable methods of virus universal detection are essential for fighting against bluetongue. We have therefore developed and evaluated a pair of primers which can detect various serotypes of BTV by RT-PCR.

对BTV进行快速、可靠的群特异性检测极为重要，因此本研究设计了适用于BTV群特异性检测的一对独特引物，并进行了评价。

They were members of MADS box gene family, and which highly homologous to AGAMOUS from Arabidopsis and other known orthologues.

参照单子叶植物的AG类基因序列设计简并引物，利用RT-PCR的方法，从百合中扩增AG到同源片段，并根据扩增出的片段序列设计特异引物进行5'RACE和3'RACE的扩增，最终获得全长。

Blood were obtained for blood RT and plasma endotoxin concentration check. 6 All data underwent ANOVA analysis. Results: After 3hrs of LPS injection, 1 in hypermetabolic sepsis group, the average HR went to 334/min, the average body temperature went to 41.0℃, while in control group they were 274.9/min and 38.2℃ respectively. p=0.000, presented significant statistical differences. The average BP and breath rate in HS group were 97.1mmHg and 94.3/min , while they were 95.9mmHg and 76.4/min in control group, respectively presenting no statistical differences. 2 In HS group plasma endotoxin concentration was 0.088EU/ml while it was 0.013 EU/ml in control group presenting statistical significance, p=0.002. 3 Blood white cell ratio and total white cell counting were 82.73% and 1.90×109 in HS group, while they were 64.39% and 7.58×109 in control group presenting very significant statistical differences(p=0.001). There was no statistical significances in RBC counting and hemocrit between two groups.

结果：LPS持续灌注3h后，结果如下：1）高代谢脓毒症组的心率升高至334.0次/min、体温升高至41.0℃，而对照组则分别为274.9次/min和38.2℃，两组间有显著的统计学差异，p=0.000；血压在高代谢脓毒症组是97.1mmHg，而对照组为95.9mmHg，两组间无统计学差异；脓毒症组的呼吸加快至94.3次/min，对照组则提高至76.4次/min，两组间无统计学差异(p=0.212)；2）其血浆内毒素含量为0.088EU/ml，而对照组为0.013 EU/ml，两组间有显著的统计学差异，p=0.002；3）血中性粒白细胞比值升高至82.73%，对照组则为64.39%，两组间有显著的统计学差异（P=0.001）；白细胞总数则明显下降至1.90×109/ml，而对照组则为7.58×109/ml，p=0.001，两组间有显著的统计学差异；两组间的红细胞数及血细胞比容均无统计学差异。

To measure the area of VEGF expression in the bronchiole and pulmonary artery. To detect the expression of VEGF mRNA With RT-PCR.

采用免疫组化和逆转录－聚合酶链反应检测VEGF蛋白质和mRNA的表达。

The antibodies were purified and the purity and specificity were identified by SDS-PAGE and Western-blot,respectively.Lung tissues from five pigs with PRRS were tested with the established method,which clearly demonstrated the abundant presence of viral antigens in the cytoplasm of alveolar macrophages,and to less extent in alveolar and bronchiole epithelial cells.

SDS-PAGE和Western-blotting检测结果表明，制得的兔抗PRRSV IgG纯度较高，具有良好的特异性，可用于原位检测病猪肺组织中PRRSV抗原的分布；Envision法的检测结果显示，PRRSV抗原的阳性表达产物主要出现在肺巨噬细胞胞浆内，其次为肺泡上皮细胞和细支气管黏膜上皮细胞；通过对5份经RT-PCR检测确认的PRRS病猪肺组织进行检测，均有较高的阳性细胞表达，平均阳性细胞率为46.5%。

The expression of AtPP2like gene(lec17 and lec26)in Cucumis melo determined by RT-PCR and the callose deposition in phloem measured by aniline blue staining were both induced by HrpNEa.

利用反转录PCR的方法，检测了甜瓜两个AtPP2类似蛋白的编码基因lec17和lec26，受到HrpNEa的诱导表达增强，同时通过苯胺蓝染色鉴定了胼胝质在HrpNEa处理的甜瓜植株中沉积增加。

The expression of AtPP2like gene (lec17 and lec26) in Cucumis melo determined by RT-PCR and the callose deposition in phloem measured by aniline blue staining were both induced by HrpN.

利用反转录PCR的方法，检测了甜瓜两个AtPP2类似蛋白的编码基因lec17和lec26，受到HrpN的诱导表达增强，同时通过苯胺蓝染色鉴定了胼胝质在HrpN处理的甜瓜植株中沉积增加。

Objective To evaluate the reliability of reverse transcription -PCR in detecting the expression of the carbonic anhydrase Ⅸ gene in renal cell carcinoma.

目的评价RT-PCR法检测碳酸酐酶Ⅸ基因在肾癌组织的表达的可靠性。

RT-PCR and Western blot were used to detect the gene/protein expression of cathepsin K.

用不同浓度的cathepsin K抑制剂E-64进行干预，油红染色评估效果。

Based on the conservation of miRNAs sequence, we compared the known miRNAs among five mam-mals, i.e., human, mouse, cattle, pig and dog with the sequence of sheep genome that is highly homologous to goat genome, published on the NCBI, and 11 candidate miRNAs were eventually obtained. RT-PCR analysis showed the expression of the 11 miRNAs in brain and 5 in liver, indicating that they might be novel miRNAs. The methodology provides an alternative approach to the exploration of new miRNAs in goat.

根据miRNA分子具有一定的保守性，文章将人、小鼠、牛、猪和狗5种哺乳动物已知的miRNA分子与NCBI公布的与山羊具有极高同源性的绵羊基因组序列对比，获得11条miRNA候选分子，然后通过逆转录聚合酶链反应验证，发现在山羊脑组织中这11条分子均有表达，肝脏组织中有5条分子表达，初步确定为山羊新的miRNA分子，为寻找山羊miRNA提供了新的思路。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷