查询词典 rt

Bp cDNA encoding L-galactono-1,4-lactone dehydrogenase fragment was cloned from fruit of Rosa. roxburghii Tratt by the method of RT-PCR on the basis of the homologous genes of Arabidopsis thaliana, cauliflower, sweet potato, strawberry, etc.

根据GenBank中登录的拟南芥、花椰菜、甘薯和草莓等植物的半乳糖内酯脱氢酶基因保守序列设计引物，采用RT-PCR法，从刺梨果实中扩增出该基因的cDNA片段855bp并测序。

RT-PCR analysis showed that CmMADS3 expressed at high levels in flowers and young fruits and at low levels in sterns.

RT-PCR分析显示，该基因在板栗的花和幼果中表达丰度高，在茎中有微弱的表达，在叶中不表达，研究结果表明CmMADS3基因是板栗花器官发育中具有E功能的功能基因。

Reverse Transcription polymerase chain reaction with RNA PCR Kit,the product of RT-PCR was determined by electrophoresis of agarose gel (100v, 30s ),the result was shoot by GDS gel imaging system, the staps were scaned and quantitated by computer analysis system.

用RNA PCR试剂盒进行逆转录聚合酶链式反应，RT-PCR产物在1.2%琼脂糖凝胶上电泳(100V，30 min)，用GDS凝胶成像系统拍摄电泳结果，各带用计算机图像分析系统扫描定量。

Negative electrophoretic results of RT-PCR products of spleens by TL-6 and IL-2 primers were observed in 2 groups.

小鼠脾组织总RNA以IL-6和IL-2两种引物进行RT-PCR，两组IL-6、IL-2均表达阴性。

Indeed Skyjack abandoned its venerable differential drive system on its 68RT line to make an RT scissors with 32 feet of platform height, for example, retract down to a 69-inch stowed height.

商业建筑的诱惑力非常大，以致于大多数主要剪式升降机制造商- Genie、Haulotte、MEC和Skyjack都在过去的18个月里引入了新型RT剪式升降机。

The summary results are below:1. GUS expression under the driving of the BjCHI1 promoter (-1060/+17) was essentially undetectable in the young seedlings under normal growth conditions. GUS activity was first detected in the stigma of young flowers, peaked in the young siliques, and decreased when the siliques became older. No GUS expression was found in the mature siliques, seeds or root.2. The BjCHI1 promoter (-1060/+17) was inducible by NaCl, PEG, wounding and MeJA treatments. High levels of GUS expression were detected in the transgenic tobacco and Arabidopsis plants after wounding, NaCl, PEG, and MeJA treatment, indicating that the BjCHI1 promoter responses to both biotic and abiotic stresses.3. RT-PCR analysis confirmed that the expression of the BjCHI1 gene in B. juncea was inducible by PEG and NaCl.4. The transcription start site was determined by 5′-RACE, and was located at the 17th nucleotide upstream of the translation initiation codon of the BjCHI1 gene.5. A -805/+17 promoter fragment was enough to response to wounding and MeJA induction, which was proved in transgenic tobacco and Arabidopsis plants. The 397 bp region between -805 and -409 of the BjCHI1 promoter contains a cis-acting element that is essential for the wounding and MeJA inducibility.6. The -695/-620 region was necessary but not sufficient to confer MeJA-responsive expression. A T/G-box locates in -353 play an important role in the expression of the BjCHI1 gene in response to MeJA treatment. The 76 bp region is coupled with the T/G-box to confer full MeJA-inducible transcription of the BjCHI1 gene.

主要结果如下：1、利用转基因拟南芥植株分析表明，正常生长条件下，BjCHI1启动子(-1060/+17)驱动GUS基因主要在花柱中表达，幼嫩的荚也有表达，并随着果荚的成熟而减弱，成熟的果荚、种子和根没有显示GUS活性。2、BjCHI1启动子(-1060/+17)能驱动GUS基因在转基因烟草和拟南芥中响应伤害的诱导，转基因拟南芥的分析还证明BjCHI1启动子也受MeJA、NaCl和PEG的诱导，证明BjCHI1启动子是一个伤害、MeJA、NaCl和PEG等生物和非生物因素诱导启动子。3、RT-PCR进一步证明芥菜中BjCHI1基因也受NaCl和PEG的诱导表达。4、5′-RACE法鉴定了BjCHI1启动子的转录起始位点，位于翻译起始位点ATG上游第17个碱基A.5、转基因烟草和拟南芥分析证明，-805/+17的启动子片段足以响应伤害和MeJA的诱导，-805和-409之间397 bp的启动子片段含有对伤害和MeJA诱导必要的元件。6、本明烟叶片瞬时表达系统分析证明，一段76 bp的序列(-695/-620)对BjCHI1启动子响应MeJA的诱导是必要的，但不足以响应MeJA的诱导，位于-353的T/G-box也参与MeJA的诱导。76 bp的序列(-695/-620)与T/G-box协同起作用，赋予BjCHI1启动子MeJA诱导性。

BaPAL gene sequence was submitted to NCBI under the accession number FJ849059. Semi-quantitative RT-PCR was applied using roots, stems, leaves, flower buds, open flowers and siliquas cDNA as PCR templates, respectively.

半定量RT-PCR结果表明，BaPAL基因在茎、叶、花蕾、开放花朵和嫩角果中均有表达，而且以开放花朵的表达量最大，根中未能检测到。

Expression of PDGF mRNA in cortex, hippocampal, basal regions and ves- sels of CVS rats were studied by RT-PCR.

采用RT-PCR技术检测了大鼠PDGF mRNA在皮层、海马、基底节区及血管转录水平的动态变化。

RT comes with many standard scrips, and you can define your own.

RT 带有很多标准的 scrips，并且您可以定义您自己的。

The 1 095 bp fragment of f3h gene of anthocyanin biosynthesis was cloned from fruit peel of riped Red Bartllet pear by RT PCR method.

利用 RT-PCR方法从红巴梨成熟果皮中克隆出花青素生成相关基因 f3 h的全长片段(1 0 95 bp)。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷