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In addition, RT-PCR analysis was used to elucidate the relationships with sugar accumulation and ShSUT4 expression levels in variable internodes.

又对不同含糖量的甘蔗品种的成熟茎节进行ShSUT4 RT-PCR表达分析，结果表明，ShSUT4表达量也与甘蔗品种的糖含量呈正相关。

The expression of IGF-IR in the difference stages (proliferative stage and involuting stage) of hemangiomas was measured using SP immunohistochemical method and RT-PCR, comparing with vascular malformation and normal skin tissues at same time.

采用免疫组化SP法和半定量RT-PCR法检测不同分期血管瘤中IGF-IR的表达水平，并与血管畸形及正常皮肤标本进行比较。

Using testis cDNA as template , amplified the KRAB fragment of SSX4 gene with RT- PCR.

以正常睾丸cDNA为模板，用RT-PCR技术扩增SSX4基因KRAB区片段，将该片段插入表达载体pMAL-C2中，并通过蓝白斑实验、酶切、PCR和DNA测序筛选和鉴定阳性克隆。

The protein and mRNA expression of PLCγ1 was examined by Western blotting and RT-PCR, and the effect of the lentivirus on cell apoptosis was analyzed by flow cytometry.

应用Western-blot和RT-PCR对细胞中PLCγ1的蛋白和mRNA表达水平进行分析。应用流式细胞仪分析PLCγ1 siRNA对细胞凋亡的影响。

The expressions of ERRα in different tissues and the transcriptional responses of ERRα in testis of medaka exposed differential EDCs were studied by quantitative real-time RT-PCR.

本研究克隆了青鱼ERRα mRNA全序列，并通过实时定量RT-PCR方法对其在不同组织中的表达和不同EDCs暴露下的响应进行了分析。

Methods: The total RNA extracted directly from rodent lung tissues which was determined to be hantavirus antigen positive by IFA methos and the hantavirus S gene segment was amplified by RT-PCR methods and the product was purified and cloned into T vector, then the sequence was analyzed.

提取经免疫荧光检测证实为汉坦病毒抗原阳性的鼠肺组织的总RNA，经RT-PCR扩增汉坦病毒S基因片段，其产物纯化后与T载体连接并进行序列分析。

Realtime RT-PCR and Western blot were used to test the expression of NSBP1,cyclinB1 and Bcl-2 in the xenografts.

运用实时RT-PCR和Western blot方法检测移植瘤中NSBP1、cyclinB1与Bcl-2的mRNA和蛋白的表达。

Branches, leaves and phloem of apple collected from an old orchard in Yanji, Xinjiang were used for total RNA isolation specific fragments of Apple fruit crinkle viroid were amplified by RT-PCR using the primers disigned according to the sequence AB104558 on GenBank. Ten sequences were obtained through recovering, cloning and sequencing, and also registered in GenBank accession No.

采集新疆焉耆地区的老果园中的苹果树枝条、叶片和韧皮部，提取总RNA，根据GenBank中的AB104558序列设计引物，利用RT-PCR技术扩增出苹果皱果类病毒（Apple fruit crinkle viroid ，AFCVd）特异条带，通过回收、克隆和测序，结果表明，获得的10条序列，已在GenBank中登录（登录号：EU031507～EU031516）。

Papaya ringspot virus; seed-borne vims; RT-PCR; molecular detection

番木瓜环斑病毒；种传病毒； RT-PCR；分子检测

Objective:The aim of the experiment is to search for the regulation that the hepatic stem cells abnormally differentiate into cancer cells. In the experiment,the variating regulation of PCNA,c-kit and c-myc in the formation and development of HCC will be interpreted with immunohistochemistry and RT-PCR. So,the initial mechanism that hepatic stem cells abnormally differentiate into cancer cells from the levels of cell,protein and gene will be clarified. The last,the inhibition to HCC with ulinastain will be studied,from which,a new way of clinical prevention and treatment for HCC will expect to be explored.

二、目的本实验旨在探讨以卵圆细胞为代表的肝干细胞在HCC发生、发展过程中在肝组织内分布规律，并通过免疫组化、RT-PCR等技术阐明PCNA、c-kit、c-myc在HCC发生、发展过程中的动态演变规律，从细胞、蛋白、基因三个水平初步研究卵圆细胞向癌细胞异常分化的机制，同时观察乌司他丁对HCC发生、发展的抑制作用，为肝癌的临床预防和治疗探索新的途径。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷