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The expression and clonality of TCR Vαand Vβsubfamilies within induced Tcells were analyzed by using RT-PCR and genescan technique.

收集诱导后第10天和第18天的细胞，RT-PCR-基因扫描分析其TCR Vα和TCR Vβ基因谱系变化特点。

Methods Recombinant adenovirus GDF-5 (Ad-GDF-5) containing green fluorescent protein and Ad-GFP were amplifiedand tittered. hBMSCs at passage 3 were infected with two viruses at different titers. At 2 days after intervention, GFP expression was observed using fluorescence microscope, and GDF-5 expression in hBMSCs was detected by RT-PCR.

将重组腺病毒GDF-5（adenovirus GDF-5，Ad-GDF-5）和空载腺病毒Ad-GFP 进行扩增并测定滴度，并以不同滴度两种病毒感染第3 代hBMSCs，干预后2 d 荧光显微镜下观察GFP 表达情况，RT-PCR 检测GDF-5 在hBMSCs中的表达。

The RT-nPCR can be used to differentiate pigs infected with wild-type CSFV from those vaccinated with the C-strain vaccine. It thus minimizes the possibility of miss-killed vaccinates during outbreaks.

本研究建立的RT-nPCR可以有效区分猪瘟强毒和弱毒，减少了未感染的免疫猪被误杀的可能性。

Mhods RT-PCR technique was used to assay the expression of MyoDI mRNA in peripheral venae blood from patients of gastrointestinal carcinoma and benign disease, 30 cases in each group.

采用RT-PCR方法检测30例胃肠恶性肿瘤恶液质和30例胃肠良性病变营养不良患者静脉血中MyoDImRNA表达。

RT-PCR was used to detect the mRNA expression of p16 and MGMT before and after treatment with 5-Aza-dC, respectively. Results: Promoter hypermethylation of the p16 and MGMT gene were detected in MGC-803 cells, and p16 and MGMT were absent before treatment.

应用5-Aza-dC处理体外培养的胃癌细胞MGC-803后，甲基化特异性PCR法检测用药前后细胞中p16和MGMT基因的甲基化状态，RT-PCR法检测用药前后细胞中p16和MGMT mRNA表达的变化。

Therefore it is interesting to clone genes related to lemma and palea. In this thesis, six genes from the lemma/palea related gene pool which is established by Liu (2003) were further studied. Those genes are salT gene (salt-induced protein), GA-SPY gene (gibberellin action negative regulator SPY-related protein), U2AF gene (U2 snRNP auxiliary factor, small subunit-related protein), kinesin-like gene, DnaJ-like gene, and EF hand gene. At first, the gene expression in leaves and 1~4 cm inflorescences of normal lemma/palea, smaller lemma/palea, and stunted lemma/palea was compared by Real Time RT-PCR.

从刘(2003)所建立水稻颖花发育相关候选基因库中，挑选6个基因：salT基因(salt-induced protein)、GA-SPY基因(gibberellin action negative regulator SPY-related protein)、U2AF基因(U2 snRNP auxiliary factor，small subunit-related protein)、kinesin-like基因、DnaJ-like基因、及EF hand基因，以水稻正常型颖花、小颖花突变体及内外颖退化突变体之叶片及1~4公分幼花序为材料，利用Real-Time RT-PCR分析，发现salT基因在突变体幼花序中大量表现。

At the same time, in situ RT-PCR of AFCVd for further detection proved the presence of AFCVd in apple leaf, mainly located in nucleus of mesophyll cell.

并利用原位RT-PCR技术做进一步检测，证明苹果叶片中有苹果皱果类病毒存在，主要分布在叶肉细胞的细胞核中。

Complementary DNA sequence of bastard halibut prion protein encoding gene was cloned and characterized through RT-PCR approach.

采用RT-PCR方法分离牙鲆蛋白质感染因子蛋白编码基因序列。

Methods In 488 cases of thyroid disease, 1% methylene blue 1.0-2.0mL was injected into the thyroid nodule or surrounding thyroid tissue 24 hours before operation. The dye-stained lymph nodes were detected at operation and RT-PCR method was used to determine MCUI in the clinically blue-stained lymph nodes.

对488例甲状腺疾病手术患者于术前24h用1%美兰1.0~2.0mL注射于甲状腺结节或周围腺体，术中显示蓝染淋巴结，采用RT-PCR法测定临床蓝染淋巴结中的MUC1。

At the same time,the mRNA levels of syk,Jak and Tnfsf13b were lower than the control by RT-PCR assay,but those of IFN-γ,IL-2 and IL-10 were higher.

RT-PCR结果提示：试验组syk，Jak和Tnfsf13b的mRNA表达水平降低，IFN-γ、IL-2和IL-10的mRNA表达水平升高。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。