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The expression of Pax-6 gene in the different stages was demonstrated by RT-PCR (Real - Time PCR):(1) The mRNA of Pax-6 gene expression was positive in Bufo raddei Strauch embryo at 16 stage; and the gene expression was also detected at 20 stage.

2荧光定量PCR结果显示在花背蟾蜍的晶状体再生的第7天Pax-6基因的表达量达到最高，表明此时期Pax-6基因在晶状体再生的过程中起到调控细胞增殖的作用。

ASES is 59% identical to Artemisia cyclase cDNA clone cASC125, 50% identical to epi-cedrol synthase from A. annua, 48% identical to amorpha-4, 11-diene synthase from A. Annua, 39% identical to the 5-epi-aristolechene synthase from tabacco, 38% identical to vetispiradiene synthase from H. Muticus, 41% identical to the δ-cadinene synthase from cotton. The coding region of cDNA was cloned into procaryotic expression vector pET30a and overexpressed in E. coli BL21 (DE3). The cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction. The tissue specific expression patterns were analyzed by RT/PCR.

克隆的倍半萜合酶氨基酸序列与烟草马兜铃烯合酶、莨菪岩兰螺旋二烯合酶、棉花杜松烯合酶的一致性分别为39％，38％和41％；与青蒿柏木脑合酶、紫穗槐二烯合酶和一个推测的倍半萜合酶克隆cASC125的一致性为50％，48％和59％。cDNA编码区序列被克隆进原核表达载体pET-30a，并在大肠杆菌BL21（DE3）中诱导表达，但过量表达的蛋白主要是以不溶性蛋白形式存在。

Morphological change of cells was observed by microscope,α-MHC and β-MHC, used as the markers of cardiogenesis expression of genes during the differentiation, were determined by RT-PCR.

结果表明，P19细胞经诱导后形态发生明显变化；α-MHC在诱导分化6d时开始表达，β-MHC在诱导分化8d时开始表达，表明心肌分化成功。

The specific primers were designed according to published sequences of PRSV CP genes of Chinese Sm strain in Genebank to perform a RT-PCR, total RNA extracted from PRSV-infected samples,Carica papaya L.cv.

根据Genebank发表的PRSV中国Sm株系的外壳蛋白基因序列，设计特异引物，以美中红（Carica papaya L。

Coli.Methods The cDNA encoding CP was amplified by RT-PCR using the mRNA extracted from premature Carica papaya as a template,and inserted into vector pET22-b.

方法从生番木瓜中提取mRNA后进行反转录，得到木瓜凝乳蛋白酶的cDNA，PCR产物克隆到pET22-b载体中，重组质粒转化至大肠杆菌BL21，进行诱导表达及表达产物的检测。

METHODS: The expression of MMP-2 in of the pulp of the healthy and carious tooth were examined by immunohistochemical staining, RT-PCR, real-time PCR and western-blot.

以健康、浅龋和深龋牙齿中牙髓组织为研究对象，利用免疫组化、RT-PCR、实时荧光定量PCR和Western印迹检测MMP-2在其中的表达差异。

Depending on the aims of investigations, cathodical charging at RT or gas phase charging at elevated temperatures were adopted.

本文研究氢在亚稳β、近β和α+β两相钛合金中的行为和氢对合金的组织结构和相变过程，以及对合金的室温和高温力学性能的影响。

We localized MMP9 expression in ceil type of the human prostate by using the method of primary cell cultures combined with RT-PCR.

为了深入了解前列腺肿瘤细胞在浸润和转移过程中，与肿瘤侵袭、转移能力相关的异常基因的表达，我们采用单管半定量RT-PCR、酶谱电泳及免疫印迹技术对前列腺癌组织中的MMP-2、MMP-9，TIMP-1、TIMP-2的异常表达情况，在基因及蛋白表达水平上进行了定量分析。

However, it is not known how orthodontic force influences the function of cementoblast and what is the relationship between the function change of cementoblast with root resorption. So we studied the effect of mechanical stimuli on root resoption by examining the expression OPG and RANKL and exploring the related signal transduction pathway in cementoblast. Methods: Cementoblasts OCCM30 were cultured in DMEM with 10% FBS for 48 hours and starved in DMEM with no FBS for 24 hours, then subjected to mechanical strain by four-point bending system with tension and compression stress at 2000μstrain at 0.5Hz frequency. The flow cytometry was used to examine the cell cycle and proliferation activity after 3h, 6h, 12h, 24h loading respectively. OPG and RANKL mRNA were analysed with real time quantitive RT-PCR after the same loading period as FCM.

本课题利用四点弯曲力学加载系统，采用流式细胞术、实时荧光定量PCR、Western免疫印迹等方法，研究体外培养的成牙骨质细胞株OCCM-30在不同力学环境中增殖活性的变化，以及其表达骨保护素和破骨细胞分化因子的mRNA的变化，探讨应力刺激对成牙骨质细胞表达破骨细胞分化调控蛋白的影响，并通过对应力刺激下成牙骨质细胞分裂原活化蛋白激酶家族中的ERK1/2、P38MAPK活性的变化及其与OPG、RANKL基因表达变化关系的研究，对力学刺激调节破骨细胞分化调控蛋白的相关上游信号转导通路进行初步探讨。

Results Multidrug resistance could be detected in the tumor tissue after the injection of retrovirus around and into it.After filtration and centrifugalization,the concentrated supernatant was 2 times efficient than the original supernatant and P-GP expression was detected in 60% of the tumor cells.mdr1 mRNA expression in the tumor tissue was significantly increased after gene transfer as detected by RT-PCR.

结果 携带mdr1基因的逆转录病毒上清液注射后肿瘤模型产生多药耐药性，经过滤和离心浓缩的病毒上清液60%细胞表达P-GP，是病毒原液基因转移率的2倍；RT-PCR表明基因转移后，肿瘤组织内mdr1 mRNA的表达量明显增加。

Hanna: That's over now, isn't it?

都结束了，对吗

You must be ill. You look so pale.

你一定是病了，你的脸色苍白。