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A novel cDNA encoding the second subunit of NADH dehydrogenase was cloned from Fraxinus velutina Torr by RT-PCR and designated as FvndhB.

采用RT-PCR 技术克隆了绒毛白蜡NADH还原酶第二亚基cDNA 序列。

At the same time, based on the extracted total RNA from the leaves of Zostera marina L., the cDNA clone could be obtained and the Na+/H+ antiporter gene could be studied by applying the techniques of RT-PCR and RACE. Its expression profile could be studied when applying the Northern technique.

同时在所成功提取出的大叶藻叶片组织的总RNA基础上，做出cDNA克隆，再通过RT-PCR和RACE技术等研究方法，来试图探究大叶藻的Na~+/H~+逆向转运蛋白基因，然后可结合Northern技术来研究其表达情况。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

Methods RT-PCR was used to detect the expression of Aven mRNA in 55 cases ofchildhood ALL. The control group included 11 childhood patients with no malignanthematological diseases.

方法应用RT-PCR检测55例儿童急性淋巴细胞性白血病患者Aven基因mRNA的表达水平，11例非恶性血液病患儿为对照组。

We observed the changes of the proprioceptors in the partly injuried ACL and the xenogeneic graft/artificial graft being used to reconstructing the injuried ACL with gold chlorid staining method and laser copolymerization electron micrography method to observe the morphological changes of the neuromechanism in the ligament tissue,with RT-PCR method to evaluate the proportion and the regeneration of the nerve component and with HRP retrograde tracking technology and electrophysiologic study to judge the functional changes of the neuromechanism in the ligament tissue,respectively.

本研究分别制作兔ACL部分损伤动物模型、异种肌腱移植物重建损伤ACL动物模型和LARS人工韧带重建损伤ACL动物模型，分别于术后不同时间点对损伤ACL/移植物进行氯化金染色光学显微镜检查和PGP9.5免疫荧光染色后激光共聚焦电子显微镜检查观察韧带内神经结构形态学变化，用RT-PCR法检测韧带中GAP-43和PGP 9.5的mRNA表达情况以评价损伤ACL/移植物内神经成分比例及神经再生情况，通过HRP逆行追踪技术和电生理检查(包括体感诱发电位和腘绳肌肌电图)评价损伤ACL/移植物内神经结构的功能情况。

Amino acid similarities between goat and human were 87% and between gaot and mouse were 83%. Just like other members of FGFs superfamily, FGF5 protein also has a signal sequence ,at same time its protein has higly similar sequence about 120 Amino acid in central core sequence with other breed. Tissue specific expression of the FGF5 gene was also investigated in cashmere goat by RT-PCR analysis. The results showed that FGF5 gene was expressed in different time of the tested skin tissues, including anage and telegon.

结果如下：(1)本研究利用 RT-PCR 方法克隆了羊 FGF5 基因的 cDNA 序列，得到了 925bp的序列，并对该序列进行了分析，与成纤维细胞生长因子家族的成员一样也是由三个外显子组成，也具有一信号序列，成熟蛋白为 270 个氨基酸，经同源性比对得出大鼠与小鼠的 FGF5 氨基酸同源性最高为 97%，在与羊的蛋白质比较中大鼠与羊的同源性为 83%，小鼠与羊的同源性为 84%，人与羊的同源性最高，为 89%。

EMMPRIN mRNA and protein expression were assayed by RT-PCR and Western blot.

应用RT-PCR和Western blot测定巨噬细胞、泡沫细胞中EMMPRIN基因和蛋白的表达。

Furthermore, the expression of bFGF and angiogenin was examined by RT-PCR and Western blot assays.

接着用RT-PCR和Western blot的方法检测了bFGF和血管生成素的表达情况。

Methods The animal model of skin avulsion injury was replicated, and the expression of CD18mRNA in avulsed skin tissues was detected by RT-PCR method; and the amount of neutrophilic granulocyte in tissues was detected by MPO method in order to analyze the tendency of neutrophil of avulsed tissues after trauma.

复制猪皮肤撕脱伤模型，采用RT-PCR方法检测伤后不同时间撕脱组织中CD18mRNA的表达；用髓过氧化酶（Myel operoxi daBe enzyme，MPO）法测定组织中中性粒细胞数量，研究伤后撕脱组织中中性粒细胞的趋化情况。

By means of RT-PCR to measure the expression of DTA in human breast cancer ceils.

采用RT-PCR法检测DTA在乳腺癌细胞中的表达，通过MTT法测定PGL3-DF3-DTA在体外对乳腺癌细胞生长的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。