查询词典 rt

It's suggested that the genes carrying DFR-1、OsMlo-1 might play a role in disease resistance of rice. Based on the putative exons of DFR-1 and OsMlo-1, primers were designed. The experiment had been performed by RT-PCR to detect the expression of gene carrying DFR-1、QsMlo-1 in rice uninoculated, inoculated with Xanthomanas oryzae pv.

为了进一步研究水稻DFR-1、OsMlo-1所在基因的功能，在DFR-1、OsMlo-1假定的外显子上设计引物，通过RT-PCR技术，研究在接种白叶枯病菌株PX099以及接种稻瘟病菌株V86013前后，水稻品种IRBB13和水稻品种明恢63中DFR-1、OsMlo-1所在基因的表达。

The expressions of VEGF、bFGF、IL-1βin myocardium were measured by RT-PCR and ELISA. Other steps and methods were same with the second part. Results: 1. The cells in 2Gy, 4Gy groups and unirradiated group grew well. All cells in 8Gy and 12Gy groups were dead after irradiation.

30只大鼠随机分为注射上清液组和培养基组，除了采用RT-PCR和ELISE两种方法检测VEGF、bFGF、IL-1β在心肌组织内的表达外，其他实验步骤、方法及检测指标同实验第二部分。

Whereas in fibroblast cell, unmethylated bands was observed.Conclusions:1. The mRNA expression of ASPP gene family was measured by RT-PCR for the first time.

1。国内首次用RT一PCR方法检测ASPP基因家族mRNA的表达，并发现ASPP基因家族mRNA在所有肿瘤细胞株中均有表达。

The promoter and partial GAG domain of CFG1 element are unmethylated, however, the partial RT domain is highly methylated.

CFG1元件的启动子和部分组特异性抗原域处于未甲基化状态，而部分RT域则处于高度甲基化状态。

Real time RT-PCR analysis showed that in compatible interaction between Pst and wheat, PsCdc2 was up-regulated at early stage of infection. The maximum induction occurred at 12 hpi, at which transcripts were 1.62 fold over that in urediniospore. From 24 to 268 hpi, the accumulation of transcripts decreased steadily.

实时荧光定量RT-PCR结果表明，在亲和组合中，该基因在条锈菌接种小麦的前期上调表达，其中接种后12 h时表达量最高，约为夏孢子中表达量的1.62倍，接种后24-268 h，基因表达基本呈下调趋势，其中96 h基因表达量最低，仅为夏孢子时期的0.07倍。

Methods The expression of XIAP in sixty cases of TCCB and ten cases of nontumorous urocystic tissue were detected by RT-PCR and immunohistochemistry.

方法应用RT-PCR和免疫组织化学法，检测XIAP在60例膀胱移行细胞癌和10例非肿瘤膀胱组织中的表达情况。

Methods 200mL fresh midpiece urina sample was collected from each of the 53 patients of transitional cell carcinoma of bladder, 20 patients of other uropoiesis disease, and 10 healthy volunteers, expression of Survivin was detected by nested RT-PCR. Voided urine cytology and cystoscopy were also conducted.

留取53例膀胱移行上皮癌、20例泌尿系其他疾病患者及10例健康志愿者新鲜中段晨尿200mL，对53例膀胱移行上皮癌患者术后癌组织，用巢式逆转录聚合酶链反应(nested RT-PCR)检测Survivin的表达，同时行尿脱落细胞学检查及膀胱镜取材病检。

Methods The expression of mTOR and P70S6K in normal cervix uteri or cervical cancer was detected by the immunohistochemistry and RT-PCR methods.

用RT-PCR和免疫组织化学法检测正常宫颈、宫颈癌中mTOR和P70S6K激酶的表达。

Meanwhile, positive human sera of HCV challenged vaccina ted monkeys after 6 weeks immunized, ALT value rose transiently in monkeys vacci nated by PCX. It was positive in monitoring RNA of HCV using RT-PCR in monkey s era.

同时，在免疫后6周用人HCV阳性血清攻击猴子，免疫PCX的猴子出现一过性ALT升高，在攻击后三周内用 RT－PCR检测到猴血清内HCV的RNA阳性。

Thedegenerate primers were designed and synthesized according to the sequence of NDHB amino acid published in GneBank. A novel cDNA encoding the second subunit of NADH dehydrogenase was amplified from Fraxinus velutina Torr by RT-PCR.

根据GenBank上已发表的NDHB氨基酸序列，设计合成了一对简并引物，采用RT-PCR技术对绒毛白蜡NADH还原酶第二亚基基因进行扩增，将扩增的PCR产物与pMD18-T连接后转化至E。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。