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Total RNA was isolated from the forth instar larvae of the armyworm,Mythimna separata Walker.An α and a β tubulin cDNA sequences were cloned by RT-PCR and rapid amplification of cDNA ends.

以黏虫4龄幼虫为材料提取总RNA，利用RT-PCR和cDNA末端快速扩增技术，分别扩增得到该虫的α和β微管蛋白基因的cDNA序列各1条。

At the indicated times,the influence of CDB on the circadian gene expression of mPeriod1 was sequently detected by RT-PCR.

于诱导后不同时间点，CDB与小鼠成纤维细胞NIH3T3细胞共孵育，RT-PCR检测其对近日节律核心分子mPeriod1(mPer1)基因表达的影响。

Meanwhile many cases were diagnosed to be sero positive of anti dengue virus. Dengue I virus were also isolated from five cases. RT PCR test also indicated 4 out of 8 samples contained dengue I virus, which was the same type of dengue virus found in the epidemic areas in Guangzhou at the same time of outbreak.

进一步的调查在病例的血标本中分离出5株登革I型病毒，同时用RT—PCR的方法从8份标本中检出4份登革I型病毒阳性，与同期广州市登革热流行的病毒型别一致；取患者的恢复期血清作肥达氏反应，结果抗&O&、&H&的滴度与第一份血相比，均显著下降。

The N gene protein was expressed in the CHO cells proved by IFA. The N gene was transcripted as proved with RT-PCR. CHO/CDV-N cell strain was constructed, which provided evidence for research of CDV serumal detection and gene vaccine.

然后通过磷酸钙共沉淀法转染CHO-K1细胞，通过潮霉素筛选得到阳性克隆，间接免疫荧光实验鉴定N基因在CHO细胞中的表达，并用RT-PCR方法从转录水平证实N基因在CHO-K1细胞中的表达，最终建立了CHO/ CDV-N细胞株，为犬瘟热病毒的血清学检测和基因疫苗的研制奠定了基础。

To prepare recombinant fox growth hormone, we amplified its cDNA from silver fox pituitary tissue by RT-PCR and cloned into yeast shuttle vector pPIC9K down stream of α-factor signal peptide sequence by SnaB I and Not I restriction sites. The recombinant secretion vector pPIC9K/fGH, linearized by Sal I, was transformed into histidine-deficient Pichia pastoris strain GS115 by electroporation.

为制备重组狐狸生长激素，采用RT-PCR方法，从银狐垂体中扩增fGH cDNA基因，利用SnaB I和Not I位点将fGH基因插入到酵母分泌型表达载体pPIC9K中α-因子信号肽的下游，构建成fGH基因的酵母分泌型表达载体pPIC9K/fGH，载体经Sal I酶切线性化后，通过电转移将线性化的pPIC9K/fGH转化到组氨酸缺陷型酵母宿主菌GS115中。

It indicated that mTSARG3 was involved in the meiosis by which spermatogenous cell differentiated to spermatocyte. We established mosue cryptorchidism model and disolated total RNA at different days after operation to perform RT-PCR.

为研究mTSARG3基因在精子发生中的调节作用，我们分离了小鼠不同发育阶段的睾丸总RNA进行半定量RT-PCR检测，结果显示mTSARG3基因在出生小鼠1周即有表达，在第3周时明显增高，之后保持稳定表达趋势。

Study on the variation of PAL activities in the inoculated leaves were closely correlated with the Stylosanthes resistance against anthracnose. The expression level of PAL gene in Mineirao is higher than that in CIAT2312 after inoculation with the test of semi-quantitative RT-PCR. And PAL gene had possible function of resistance to anthracnose.

本研究通过对不同柱花草种质对炭疽病的抗性鉴定，在验证了PAL 在Mineirao 柱花草抗炭疽病方面起一定作用的基础上克隆出PAL 基因，利用半定量RT－PCR 检测该基因在两种柱花草中的差异性表达，分析表明，Mineirao 表达量高于CIAT2312 柱花草，推测出该基因与抗炭疽病有关。

Using the semi-quantitative RT-PCR and northern blot methods,SCY2 transcripts were investigated in various tissues including exoskeleton,subcuticular epithelia,gills, heart,hepatopancreas,stomach,muscle,eyes,female and male reproductive apparatus.

用RT-PCR和Northern-blot等方法，分别检测了SCY2基因在锯缘青蟹不同组织器官(鳃、甲壳、眼、血细胞、肝胰脏、心脏、肌肉、甲壳下皮层、胃和生殖系统等)中的转录表达情况，发现该基因仅在雄性生殖系统中表达。

Among the bacterial strains, 5 strains of RP,RS,RT,RX and RY were suscept ib le to all 20 phage strains, so they could be used as indicative bacteria for det ecting the phage particles in checking samples in order to forecast bacterial le af s treak of rice with the bacteriophage isolation technique in Hubei Province.

根据它们相互的亲和关系，可把10个细条病菌菌株划分为6个组，把20个噬菌体划分为7个溶菌型；其中RP、RS 、RT、RX、RY等5个细条病菌菌株可与所有参试的20个噬菌体发生亲和反应，据此在用噬菌体技术进行湖北省水稻细菌性条斑病的测报工作中，这些广亲和性的菌株可作为指示菌。

METHODS: Two groups of rats ［allograft group and syngenic graft group］ underwent limbal transplantation and healthy rats were taken as normal controls. The expression of RANTES and MCP 1 mRNA was detected by quantitative competitive RT PCR at post operative days 1, 3, 7, 11, 14, 21 and 30 and rejections were observed.

将大鼠随机分为同种异体移植组、自体移植组，并以健康大鼠作为正常对照组，用定量竞争性RT PCR的方法，在术后1， 4， 7， 11， 14， 21和30 d分别检测各组大鼠RANTES和MCP 1 mRNA的含量，并观察大鼠眼表变化，判断排斥反应的发生。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。