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Methods Rattus hearts in embryonic days 12, 15, 19 were included in this study. The mRNA level of HCK gene expression was detected by the methods of RT-PCR and in situ hybridization, and the results were analyzed by the statistical methods.

方法取大鼠胚胎12、15、19d心脏，采用RT-PCR技术检测心脏组织中HCK基因mRNA的表达丰度，并应用原位杂交技术对HCK基因表达进行定位分析。

Methods: Total RNA was extracted from esophageal carcinoma tissue, the primer containing special enzyme was designed, NY-ESO-1 fragment was amplified by RT-PCR, and then was cloned into the expression vector pET-15b by LP Recco PCR Cloning. The recombinants plasmid pET-15b-NY-ESO-1 were identified by restriction endonucleases digest analysis and sequence analysis.

从人新鲜的食管癌组织中提取总RNA，通过RT-PCR技术扩增NY-ES0-1基因3'端的340bp片段，采用靶向克隆法将目的基因插入到原核表达载体pET-15b中，得到重组表达质粒pET-15b-NY-ESO-1，经过筛选，挑选出阳性克隆进行XhoⅠ和BamHⅠ双酶切图谱分析、PCR检测和扩增产物核苷酸序列分析等，鉴定所构建的原核表达载体。

Total RNA of the viral supenatants, RT-PCR analysis showed that cDNA amplifited had same legth with that of sM gene. It indicated that recomb...

提取重组病毒的RNA进行RT-PCR分析，结果表明成功获得plxas-sM逆转录病毒。

Four paired of specimens, including throat/recta swabs, cerebral spinal fluid and blood were collected when admitted; one set were sent for RT-PCR and another were for virus culture.

每一位均收集四组成对检体，包括喉头/肛门拭体、脊髓液及血液，一套以RT-PCR侦测肠病毒基因之阳性与否；另一套则以传统病毒培养而侦测之。

Both groups were detected diopter using retinoscopic refraction, determined eyeball axis using ophthalmology ultra-A, and investigated the dynamic expression of VIPmRNA on posterior eyeball at three stages by RT-PCR.

两组均采用检影验光检测屈光度：眼科A超测定活体眼轴长度；对两组3个时间段眼球后壁行RT-PCR检测VIPmRNA的动态表达。

The full length CMO cDNA was cloned from Salicornia europaea by RT-PCR and RLM-RACE.

本研究利用RT-PCR和RLM-RACE技术获得了盐角草的CMO cDNA全序列。

Ve schizophrenic inpatients will be enrolled into the 6-week, randomized, double-blind trial of sarcosine monotherapy (1000~3000 mg/day, N=75) vs. risperidone (2~6 mg/d, N=75). We will measure clinical efficacy and side effects biweekly during the treatment. At the beginning and the end of the trial, we will also measure cognitive function (e.g. intellect, attention, memory, executive function), serum amino acids, expressions of mRNA (by realtime quantitative RT-PCR) and protein of NMDA-related genes (such as SHMT2, AMT, PSAT1, ASCT1, and serine racemase) in peripheral blood lymphocytes.

第一部份将收案150位未曾治疗之精神分裂症住院患者，以双盲方式随机给予六周sarcosine (1000-3000 mg/day，N=75)或risperidone (2-6 mg/day，N=75)治疗，我们将每两周评估临床疗效与安全性，并在研究开始及结束时，评量认知功能(智能、注意力、记忆力、执行功能)并测定血清中glycine或D-serine浓度以及SHMT2、AMT、PSAT1、ASCT1、serine racemase等基因於周边淋巴球之mRNA (以realtime RT-PCR测定)与蛋白质表现量。

To further investigate mechanism of BnMPK4 in Resistance to Sclerotinia Sclerotiorum, analysis of expression changes of defense-marker genes were carried out by quantitative RT-PCR.

为了研究BnMPK4转基因植株的抗菌核病机理，采用定量RT-PCR调查转基因植株中植物防卫标记基因的表达变化。

Derstand PG signaling pathway, PG gene was cloned from Sclerotinia sclerotiorum by RT-PCR, the encoding region of PG was

关。为了解PG 的信号转导途径，利用RT-PCR 方法克隆了核盘菌的PG 基因，将PG 的编码区与酵母GAL4 的DNA

RT-PCR technique was applied to detect the expression of PAF receptor gene in ischemic semidark band cortex after MCAO and recanalization. At the same time ELISA method was used to determine the corresponding PAF values in plasm.

应用RT-PCR技术检测MCAO及再通后缺血半暗带皮质PAF受体基因表达，同时用ELISA检测对应血浆PAF值。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。