查询词典 rt

The results showed that: 1 under the hydroponic culture established in this research, the experimental wheat plants grew well andperformed the typical architecture responses when exposed in Pi deprivation. Hemi-qantitative RT-PCR revealed that the transcriptional level of known P response gene changed dramatically; 2 In general the length of wheat root axis and the root/ shoot ratio arose when the Pi supply was deprived, however, the change models differed among different wheat genotypes. The investigation on their molecular basis would offer the useful clue for seeking high phosphorus efficiency protoplasma and gene resources of wheat.

结果表明：1在研究建立的小麦水培体系中，小麦植株生长正常并对磷饥饿胁迫条件做出发育形态建成水平的反应；半定量RT-PCR分析显示在此体系中小麦磷响应基因也在转录水平上产生显著变化；2在水培条件下不同基因型小麦对磷饥饿胁迫的反应总体上表现在根轴长度和根冠比有所提高，但不同基因型小麦的反应特点有差异；对这些反应特征的分子机理的深入分析可为有效筛选磷高效小麦新种质以及发掘磷高效基因资源提供线索。

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法，将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中，对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测，对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白，并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测。

From May 2005 to April 2006,annual distribution of Citrus psorosis virus on the leaves and shoots of Dweet tangor seedlings was detected by using one-step RT-PCR once a month.

2005年5月到2006年4月逐月采样，运用一步法RT-PCR检测 Citrus psorosis virus在Dweet橘橙苗木叶片和枝皮中的分布。

Methods Pterygial samples were extracted and collected and the pterygial endothelial cells and pterygial fibroblasts were cultured alone, conditional and co-cultured to form different culture systems. The methods included that to select the suitable intensity of ultraviolet by MIT, to detect the changes of curves of growth about two kinds of cells by MIT and to explore the developments of protein and RNA of vascular endothelial growth factor and fibroblast growth factor-basic in three culture systems under ultraviolet whose intensity is 20 mJ/cm^2 by ELISA and RT-PCR.

收集翼状胬肉标本，采用血管内皮细胞和成纤维细胞单独培养、条件培养和共同培养的方法构建体系，用MTT法选择紫外线照射细胞的适宜强度；采用MTT法绘制强度20mJ/平方公分紫外线照射下细胞生长曲线；采用ELISA和RT-PCR检测紫外线照射下3种体系中细胞上清液和细胞中血管内皮细胞生长因子和成纤维细胞生长因子的蛋白和RNA含量变化。

The biological effect of pUCP18/lasR was detected by RT-PCR, NAD method and the assay of pyocyanin.

RT-PCR检测LasB基因和LasI基因mRNA的表达，NAD法测定外毒素A的活性，紫外分光光度计测定绿脓菌素的产生水平。

Objective To study the relationship between expression of peripheral blood survivin and prognosis of breast cancer patients.Methods Expression of peripheral blood survivin of breast cancer was determined by the methods of RT-PCR、quantitied PCR and immunohistochemistry.

目的 探讨乳腺癌患者外周血中生存素表达的情况与预后的关系方法应用RT-PCR、定量PCR、免疫组化法检测，检测10例晚期乳腺癌患者外周血及组织中生存素的表达。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

In this study, a rab gene was obtained from Euplotes octocarinatus by polymerase chain reaction and RT-PCR.

本实验运用PCR、RT-PCR等技术，从八肋游仆虫中克隆到一种新的rab基因。

RT-PCR showed that the opening reading frame of the rab gene was 663 bp long.

通过RT-PCR，从mRNA获得的该基因的开放读框为663 bp，表明该基因在转录过程中有内含子的删除。

The expression of Sox genes in different tissue of Rana rugulosa Wiegmann was analysed by using RT-PCR technique.

采用RT-PCR技术研究了虎纹蛙不同组织中Sox基因的表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。