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METHODS:The techniques of RT- PCR and Northern Blot were adopted to test the gene expression of OB- R and the radioimmunoassay was used to test the serum a nd hypothalamus leptin and insulin in order to observe the changes of serum lept in, insulin and lipide in simple obesity patients and the changes of weight,Lee' s index,somatic fat,the contents of serum and hypothalamus leptin and insulin as well as the expression of hypothalamus OB- R gene in obesity rats before and a fter acupuncture treatment.

采用RT-PCR和NorthernBlot技术测定瘦素受体基因表达水平，放射免疫分析测定血清和下丘脑瘦素和胰岛素的含量。

To investigate the mechanism of difference in intramuscular fat deposition between Erhualian and Large White pigs, single tube relative-quantitative RT-PCR method was used to investigate the development patterns of lipogenic and lipolytic gene expression with 18S internal standard control.

采集背最长肌，用乙醚抽提法分析肌内脂含量，同时取冈上肌和半膜肌提取组织的总RNA，用相对定量RT-PCR方法，以18S作内标，分析脂肪合成基因与水解基因表达的发育性变化。

To study the transcription expression law of Musclin gene and Lipogenic gene in muscle of the cadmium poisoning mice regulated by Vc. The experiment adopted RT-PCR detection method to study the variety of expression level of mice Musclin gene and Lipogenic gene (FAS, PPARγ, TGH).

为探讨维生素C调控Cd中毒小鼠骨骼肌中Musclin基因及生脂基因的转录表达规律，用RT-PCR方法研究小鼠Musclin基因及生脂基因(FAS、PPARγ、TGH)表达水平的变化。

With 〓H-TdR incorporation and cell count, we found that AdM could inhibit DNA synthesis and the proliferation of VSMCs from SHR. With semiquantitative RT-PCR analysis, we found that AdM mRNA level was lowerer in kidney and VSMCs from SHR than those from WKY.

应用〓H-TdR掺入和细胞计数发现，AdM可以抑制培养的自发性高血压大鼠VSMCs的DNA合成和细胞增殖；应用半定量RT-PCR分析发现，在自发性高血压大鼠的肾和VSMCs中，AdM mRNA水平较低。

These results demonstrated that BmCPV,DsCPV and LdCPV were homologous as classfied in the same electropheres type I,and that this RT\|PCR assay could be used for early,rapid,sensitive and specific detection of DsPCV infection in the natural population of the pine moth,due to the apparent different feeding habits of Bombyx mori,Dendrolimus ssp.and Lymantria dispar larva each other and the no infection of BmCPV and LdCPV to Dendrolimus ssp.

由于松毛虫与家蚕、舞毒蛾相互之间食性不同，而且BmCPV和LdCPV对松毛虫无感染性，即在松毛虫体内不会有BmCPV病毒和LdCPV病毒的感染，因此该结果一方面从分子水平上证实了DsCPV与BmCPV、LdCPV存在基因同源性，同时也表明了依据BmCPV的基因序列设计引物对DsCPV基因组核酸建立的RT-PCR扩增体系，可以作为松毛虫种群中DsCPV的一种敏感、特异、早期、快速的检测手段。

The method uses RT-PCR method to detect RUNX3 gene expression, with methylic change particularity PCR method to detect RUNX3 gene opens mover methylic change.

方法采用RT-PCR方法检测RUNX3基因表达，用甲基化特异性PCR方法检测RUNX3基因启动子甲基化。

C2C12 cells were induced to differentiation into muscle cells in vitro and the myogenesis model was detected by inverted microscope, RT-PCR, western-blot, cell immunofluorescence histochemistry, flow cytometry and confirmed to be successful. On the foundation of differentiation model, miR-206 was detected by northern hybridization and the result showed that the expression level increasing gradually during muscle differentiation with a character of phase specificity.

利用C2C12细胞在体外成功诱导其向成肌细胞分化，通过形态学观察，RT-PCR、western-blot、细胞免疫荧光组织化学及流式细胞仪等方法进行检测，证实我们所建立的细胞分化模型是成功的，并观察到随着诱导时间的延长，miR-206在成肌分化过程中表达量逐渐增高的，表达改变具有阶段特异性。

The myrosinase gene was amplified from total RNA by RT-PCR and inserted into Escherichia coli expression vector pGEX-4T-1.The result of sequencing was EF583560 in GenBank.

实验通过RT-PCR程序从提取的油菜总RNA中扩增出硫代葡萄糖苷水解酶（又称黑芥子酶，EC3.2.1.147）基因，酶解后插入大肠杆菌表达载体pGEX-4T-1，获得克隆菌株。

This study consists 3 partsPart I: the research on the mechanism of affecting the growth of CAM vesselsBecause neoformative vessels is most related with bFGF and VEGF, this study designed bFGF, VEGF and the primer of its receptor, by the experiment of the generation of CAM vessels, draw the materials from CAM tissues consist account of vessels, by the experiment of RT-PCR, reserve the influence of HSYA on the expression of bFGF, VEGF, VEGF-R(flt-1) in CAM tissues.

本实验分为三部分：第一部分，HSYA影响鸡胚尿囊膜血管生成的机理研究由于新生血管与bFGF、VEGF及其受体的关系最为密切，本实验设计了bFGF、VEGF及其受体的引物，利用CAM血管生成实验，直接从富含血管的CAM组织上取材，通过RT-PCR实验，观察HSYA对CAM组织中上述三个基因表达的影响。

Methods A total of 53 SRE specimens and 8 nomal nephridial tissues were obtained. Immunohistochemistry method was used to test protein expression of survivin. Semi-quantitative RT-PCR was used to measure survivin mRNA levels.

收集53例肾透明细胞癌组织及8例正常肾组织，以免疫组织化学方法检测survivin蛋白表达；半定量RT-PCR方法，检测肾透明细胞癌中survivin mRNA表达水平，与正常肾组织进行比较分析。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。