查询词典 rt

All the muscles rebuilt to stable structure after accomplish of distraction, which may help the post-operative stability.2. The changes of myosin heavy-chain mRNA in muscles of mastication during distraction osteogenesis of mandible in canineThe mRNA of MHCI, MHCH and GAPDH in masseter and geniohyoid were measured by RT-PCR.

试验二犬下颌骨牵张成骨过程中咀嚼肌肌球蛋白重链亚型mRNA含量的变化将所取标本应用RT－PCR对嚼肌与颌舌骨肌肌球蛋白重链的两个亚型，即MHCI与MHCll与内参照GAPDH的InRNA进行半定量检测并进行统计学分析。

The invention clones geranyl pyrophosphate synthase GGPPS gene from the ginkgo to construct plant expression vectors which contain ggpps gene, the ggpps gene is inducted into immature embryos of the ginkgo to induct out the callus tissue by the mediating of agrobacterium tumefaciens, PCR and semiquantitative RT-PCR detect the conformation and expression status of exogenous target gene ggpps, high-performance liquid chromatography and an evaporative light-scattering detector are employed to determine the terpene lactones content inside the callus tissue of the ginkgo, and the obtained callus tissue of the ginkgo of which the terpene lactones content is increased is screened.

本发明从银杏中克隆香叶基香叶基焦磷酸合成酶GGPPS基因，构建含ggpps基因的植物表达载体，用根癌农杆菌介导，将ggpps基因导入银杏幼胚并诱导出愈伤组织，PCR和半定量RT-PCR检测外源目的基因ggpps的整合和表达情况，高效液相色谱法及蒸发光散射检测器测定银杏愈伤组织中萜内酯含量，筛选获得银杏萜内酯含量提高的转基因银杏愈伤组织。

Additionally, one gene that was similar to germin was confirmed by RT-PCR analysis.

对编码germin蛋白的基因的RT-PCR结果表明，该基因在杂交种中表达量下降。

The expression characterization of gibel carp Caghira gene in different tissues and stages of embryogenesis were analyzed by RT-PCR and whole mout in situ hybridization.

采用RT-PCR方法并结合整体原位杂交的方法分析了银鲫Caghira在不同组织中和胚胎发育不同阶段中的表达特征。

Methods Ast was isolated and cultured in vitro and its model of reactive gliosis was established by scratching the cultured astrocytes. The reactivity and rules of Ast to injury was studied by morphological changes, RT-PCR, immunocytochemistry, in situ hybridization and imaging analysis.

体外分离培养Ast，利用划伤的方法，建立Ast对损伤反应的实验模型，通过形态学观察、RT-PCR、免疫细胞化学、原位杂交及图像分析等方法，研究体外Ast对损伤的反应及规律。

Abstracts: Objective To appraise the intervening effect of Shuanghuang Shadu Tuishao Gule to the NDV with fluorescence ration RT-PCR methods used to check moderate and strong NDV.

目的 应用已建立的检测中、强毒力新城疫病毒的实时荧光定量RT-PCR方法评价双黄杀毒退烧颗粒对NDV的干预作用，为抗病毒药物的快速筛选和疗效评价奠定基础。

Methods The current conservative sequence primer and probe were designed according to the gene sequence of moderate and strong NDV in GeneBank. Fuorescence ration RT-PCR was done to the special TaqMan probe of moderate and strong NDV .The proliferation of NDV in chicken embryo was evaluated after taking Shuanghuang Shadu Tuishao Gule comparing with the control group.

根据GenBank登录的NDV中、强毒力株F基因序列，选择包含裂解位点的保守序列设计通用引物和针对中、强毒力NDV的特异性TaqMan探针进行实时荧光定量RT-PCR，评价双黄杀毒退烧颗粒干预后NDV强毒在鸡胚中和在机体内病毒的增殖情况，并与未干预的对照进行了比较。

The 15 ku ES antigen gene was amplified by RT-PCR from the total RNA extracted from the Haemonchus contortus.

contortus15ES抗原基因进行原核表达，本研究利用RT-PCR技术从黑龙江省羊源H。

Partial cDNAs encoding LHR gene were cloned from Cynoglossus semilaevis by nested reverse transcrip-polymerase chain reaction (nested RT-PCR). Alignment analysis showed that the sequence was 97%, 94%, 85%, 83%, 82% and 80% similar to those of Hippoglossus hippoglossus, Paralichthys olivaceus, Acanthopagrus schlegelii, Oreochromis niloticus, Oryzias latipes and Takifugu rubripes, respectively.

采用巢式RT-PCR法克隆了半滑舌鳎Cynoglossus semilaevis促黄体激素受体(Luteinizing Hormonene Receptor， LHR)基因部分序列，经过BLAST比对，认为该序列与庸鲽、牙鲆、黑鲷、尼罗罗非鱼、青鳉和红鳍东方鲀的LHR基因同源性分别为97%，94%，85%，83%，82%和80%。

RT-PCR analysis showed that GhMADS1 gene expressed in petals, stamens, ovules and fibers, but not in roots, stems, leaves, bracts and sepals. The strongest expression of GhMADS1 gene was detected in petals. But in floral buds of a cotton homeotic mutant (CHV1), whose floral organs are all converted to bract leaf-like organs, the transcript of GhMADS1 gene was not detected.

RT-PCR分析显示，该基因在陆地棉的花瓣、雄蕊、胚珠和纤维中表达，特别是在花瓣中表达量最高，而在根、茎、叶等营养器官和棉花同源异型突变体CHV1（所有花器官均变为苞叶状叶性器官）的变异花蕾中不表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。