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We collected normal brain tissues from 10 patients who underwent decompressive surgery because of brain injury. The expression of CENP-F was detected by RT-PCR and immunohistochemical staining respectively in normal brain tissues, and these were used as control for the CENP-F expression.

3收集10例颅脑外伤后行手术减压的正常脑组织，分别采用免疫组化和RT-PCR两种方法分别检测正常脑组织中CENP-F的表达，并作为对照组。

FCM analysis showed that the apoptotic rate of MDA-MB-231 cells and intracellular Ca(superscript 2+) concentration increased gradually with the increase of deguelin concentration. RT-PCR and Western blotting analysis showed that the expression of Bcl-2 mRNA and protein was clown-regulated and that of Sax was op-regulated after deguelin treatment.

流式细胞术检测显示细胞凋亡率和细胞内钙离于浓度随鱼藤素浓度的增加而增多；RT-PCR和蛋白印迹检测结果发现鱼藤素能使Bcl-2 mRNA和蛋白表达减少，而Bax表达增加。

RT performance was severely impaired following bilateral injection of 6-OHDA into the dorsolateral part of the striatum.

结果发现，纹状体6-OHDA注射不仅严重损伤了大鼠的运动功能，并且降低了RT任务过程中对各类事件产生反应的神经元数量。

Specific gene expression of the pro-inflammatory cytokine IL-1β and the type II IL-1βreceptor (IL-1RII, 'decoy receptor') was studied in skin of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) fry during infection with the ectoparasitic monogenean Gyrodactylus derjaini Mikailo, 1975. Cytokine expression was studied in both primary and secondary infections using a semi-quantitatie RT–PCR technique.

用半定量RT-PCR的方法，研究初次感染和二次感染体外寄生的单殖吸虫---三代虫后，虹鳟鱼苗的皮肤中促炎症细胞因子IL-1β及IL-1βII型受体（IL-1RII）的特异性基因表达。

Specific gene expression of the pro-inflammatory cytokine IL-1β and the type II IL-1βreceptor (IL-1RII, 'decoy receptor') was studied in skin of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) fry during infection with the ectoparasitic monogenean Gyrodactylus derjavini Mikailov, 1975. Cytokine expression was studied in both primary and secondary infections using a semi-quantitative RT–PCR technique.

用半定量RT-PCR的方法，研究初次感染和二次感染体外寄生的单殖吸虫---三代虫后，虹鳟鱼苗的皮肤中促炎症细胞因子IL-1β及IL-1βII型受体（IL-1RII）的特异性基因表达。

Based on the trait of adsorption and desorption of chicken red blood cell with AIV under some conditions, virus enrichment was performed in 48 samples diagnosed by CRBC, and the enrichment products were detected by the multiplex RT-PCR In whih, 30 of 48 were showed to be H5 subtype AIV, completely accorded with the result of same samples tested by embryonated eggs propagation and hemagglutinin inhibition assay.

结合AIV能够吸附鸡红细胞并在一定条件下解离的特点，利用鸡红细胞对48份已确诊样品进行病毒富集，然后用建立的多重RT-PCR方法检测富集产物结果显示48个样品中，30个为H5亚型AIV阳性，与病毒分离结果完全一致。

Therefore, the object of this study is to investigate the prevalence of both enteric virus in Taiwan by molecular method (RT-PCR and ELISA).

因此，本研究的目的是以分子生物学的方法(RT-PCR和ELISA)调查轮状病毒与肠病毒在台湾的交叉流行期分布。

Using RT-PCR technique, the expression of ECRG between normal epithelia and EC was significantly different.

4通过RT-PCR鉴定，已初步证明上述4个ECRG片段在正常食管上皮组织和食管癌中的表达有差异。

Combining SSH and cDNA microarray to screen of up-regulated ESTs in the water stress Erianthus arundinaceus based on the analysis of up-regulated ESTs,a full-length cDNA sequence was cloned from sugarcane through Rapid Amplification of cDNA Ends method,and the gene expression character was analyzed by real time RT-PCR.

应用消减文库技术结合cDNA芯片技术筛选水分胁迫诱导的基因的EST序列，根据筛选到感兴趣的上调表达基因的EST序列，用RACE技术获得SSADH的全长cDNA序列，并通过实时荧光RT-PCR技术对该基因的表达特征进行分析。

Furthermore, K562 cells transfected by lentivirus plasmids were induced into the erythropoietic cells and the erythropoietic differentiation of K562 cells were examined by benzidine staining, immunocytochemistry and RT-PCR.

进一步对SOCS-3基因沉默后的K562细胞进行了诱导分化，并采用联苯胺染色法检测K562细胞向红系分化比例变化，免疫荧光染色检测细胞表面抗原的变化，RT-PCR 检测造血相关基因的变化。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。