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METHODS: The BEC were preincubated with crocetin (1, 0.1 and 0.01 μmol/L) for 12 h, then exposed to AGE (100 mg/L). The RAGE mRNA expression was detected by RT-PCR analysis, the intercellular adhesion molecule-1(ICAM-1) was measured by ELISA. The extracellular superoxide ion and thiobarbituric acid reactive substances were assessed with superoxide ion kit and colorimetric assay, respectively The intracellular I2O2 was also detected using the probe 2,7-dichlorofluorescein. The mitochondrial membrane potential and mitochondrial Succinate dehydrogenase were analyzed by the retention of rhodamine 123 (Rh123) and MTT.

不同浓度的西红花酸(1、0.1、0.01μmol/L)预孵BEC细胞12h后，用AGE(100mg/L)刺激细胞12h，RT-PCR法测定RAGEmRNA的表达水平；ELISA法测定细胞间黏附分子－(ICAM-1)的表达；试剂盒分别检测胞外超氧阴离子和硫代巴比妥酸反应产物浓度；同时，还用2，7-二氯荧光素测定了胞内H2O2的浓度，并用罗丹明123(Rh123)荧光法及MTT法分别检测细胞线粒体膜电位水平和其琥珀酸脱氢酶的活性。

The reverse transcription polymerase chain reaction RT-PCR and cDNA microarray were used to evaluate VEGF-C mRNA expression of T24 cells at around the best effect time and as the Crocin concentration reached the best.

用RT-PCR和基因芯片分别检测最佳作用时间和最佳药物浓度作用前后T24细胞VEGF-C的表达。

Methods Semi-quantitative RT-PCR was employed to analyze the polymerase β mRNA in 41 cases of epithelial ovarian carcinoma, 27 cases of serous cystadenocarcinoma, and 4 cases of mucinous cystadenoma, endometrioid tumor, and 31 cases of normal ovarian tissue.

采用含有内对照的半定量RT-PCR方法，检测41例卵巢癌患者（其中浆液性囊腺癌21例，黏液性囊腺癌13例，子宫内膜样癌7例）及正常卵巢组织31例pol βmRNA的表达。

In othor way, we use RT-PCR to observe the secretion of mRNA to compare with the balance of TH1/TH2 in spleen, so that we can see if we treat with cystamine can apparently raise the ratio of TH1 and also raise mRNA of CD25/ Foxp3, but reduce mRNA of CD40L. As a result of kidney which is the important secondary immunity organ discusses cystamin whether is the important control action or not to regulate in kidney then to be able to improve illness.

另外，透过了反转录聚合链锁反应(reverse transcription-polymerase chain reaction， RT-PCR)观察spleen中CD8/CD4 maker的mRNA表现量比较TH1/TH2 balance的倾向，发现经过胱胺处理后的确TH1相关的CD8 maker的mRNA比例量有大幅度上升，间接证明SLE倾向於TH2有被反转，而在Treg的特异性 maker方面CD25/ Foxp3之mRNA之比例也有大量上升，T cell的活性化maker CD40L也减少了mRNA的表现量，同时证实了SLE免疫倾向的反转，及发炎情况的减少及Treg cell的大量诱发。

The mice were killed 3, 7 and 14 days after operation (8 mice per group at a time), the tissue of cystiform was harvested to receive gross, histology and immunohistochemistry observation, as well as RT-PCR and Western blot detection.

术后观察动物一般情况，于给药后3、7 及14 d，每组取8 只小鼠背部囊壁组织行大体、组织学观察及免疫组织化学、RT-PCR、Western blot 检测。

The levels of SOD and CAT significantly increased and MDA decreased in bifidobacterium group compared to radition damage group.Electronic microgragh of spine neurocyte and neurogliocyte showed that cytoplasm,cytoblast and plastiosome were significantly engorged ,and neurogliocyte apoptosised in earlier period. TLR2mRNA increased in C group compared to B group by RT-PCR. Cytochrome C in kidney increased in C group compared to B group, but bcl-2,NF-Кb decreased. Conclusions : Enteroclysm is better compared to intragastric administration.

双歧杆菌处理组肝组织中SOD和CAT水平明显高于放射损伤组(P.01)，同时MDA明显低于放射损伤组(P.01)；电镜结果显示：放射损伤组与双歧处理组的细胞浆、细胞核和线粒体均明显肿胀，双歧处理组同时具有早期凋亡现象；RT-PCR结果显示：双歧处理组TLR2mRNA的表达明显高于放射损伤组；免疫组化结果显示，双歧处理组Cytochrome C的表达明显优于放射损伤组，而NF-кBp65和bcl-2则相反。

Nested RT-PCR and cytopathological examination were used to determine the CK19 mRNA and CEA mRNA expressions.

常规行H-E染色细胞学检查，采用巢式RT-PCR方法检测腹腔冲洗液中CK19 mRNA和CEA mRNA的表达，并以胃癌细胞SGC7901为阳性对照。

Total mRNA was extracted from a venom gland of snake Daboia russellii siamensis following the manufacturers protocol of the PolyATtract System 1000 kit purchased from Promega Biotech. cDNAs encoding C-type lectins were amplified by RT-PCR and subcloned into a pMD18-T vector.

按照Promega公司的mRNA提取试剂盒操作手册，从圆斑蝰蛇的毒腺中提取mRNA；利用RT-PCR的方法进行体外扩增，获得C-型凝集素蛋白的基因，克隆到pMD18-T载体中。

Methods:TNF-α and TGF-β_1 mRNA expression on placenta decidual tissue from 15 early spontaneous abortion women and 20 normal early pregnant women were examined by real-time fluorescence quantitative RT-PCR.

方法采用实时荧光定量RT-PCR法检测了15例早期自然流产及20例同期正常早孕要求行人工流产者蜕膜组织的TNF-α和TGF-β1mRNA表达水平。

The remaining 9 rats in control group, 16 rats in treated grouop and 15 rats in model group were decollated and obtained brains to detect the relative mRNA expression of nuclear receptor-related factor 1 (Nurr1) and muscarinic acetylcholine receptor 5(M5) byusing RT-PCR, which was the consequence of those comparing with reference geneβ-actin mRNA expression.

将对照组、治疗组、模型组其余9、16、15只大鼠，断头取脑，采用RT-PCR方法检测大鼠脑组织孤儿核受体及乙酰胆碱毒蕈碱型受体5(M5)mRNA相对于内参基因β-actin mRNA的相对表达值。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。