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The mRNA and protein expression of Cyclin D1 and p27 were detected by RT-PCR and Western Blotting respectively.

RT-PCR 和Western Blotting法分别检测细胞周期调节蛋白D1(Cyclin D1)和P27 mRNA和蛋白水平的表达变化。

The mRNA and protein of CD14 and TLR4 in U937 cells were detected by semi-quantitative RT-PCR and Western blotting.

RT-PCR 和Western blotting检测U937 细胞膜受体CD_(14)和跨膜受体钟

The total RNA was abstracted from the larvae of Boophilus microplus, and a 1982bp Bm86 gene was amplified by RT-PCR. The target gene was subcloned into T vector. The sequencing showed that the nucleotide sequence of the cloned Bm86 gene shared 97.4% homology with the data published in and this fragment contained the complete open reading frame of Bm86 gene.

为了克隆微小牛蜱Bm86 基因及构建该基因的表达载体，以微小牛蜱饥饿幼蜱的破解物提取的总RNA为模板，参照已发表的微小牛蜱Bm86基因的核苷酸序列，设计了1对引物，采用RT-PCR技术获得微小牛蜱Bm86基因；将Bm86基因克隆入载体，并进行序列分析，结果证明，克隆的Bm86基因序列与GenBank上登录的Bm86基因序列的同源性达97.4%，而且该序列包含完整的开放阅读框。

METHODS: The expression of MMP-2 in of the pulp of the healthy and carious tooth were examined by immunohistochemical staining, RT-PCR, real-time PCR and western-blot.

以健康、浅龋和深龋牙齿中牙髓组织为研究对象，利用免疫组化、RT-PCR、实时荧光定量PCR和Western印迹检测MMP-2在其中的表达差异。

We localized MMP9 expression in ceil type of the human prostate by using the method of primary cell cultures combined with RT-PCR.

为了深入了解前列腺肿瘤细胞在浸润和转移过程中，与肿瘤侵袭、转移能力相关的异常基因的表达，我们采用单管半定量RT-PCR、酶谱电泳及免疫印迹技术对前列腺癌组织中的MMP-2、MMP-9，TIMP-1、TIMP-2的异常表达情况，在基因及蛋白表达水平上进行了定量分析。

Results Multidrug resistance could be detected in the tumor tissue after the injection of retrovirus around and into it.After filtration and centrifugalization,the concentrated supernatant was 2 times efficient than the original supernatant and P-GP expression was detected in 60% of the tumor cells.mdr1 mRNA expression in the tumor tissue was significantly increased after gene transfer as detected by RT-PCR.

结果 携带mdr1基因的逆转录病毒上清液注射后肿瘤模型产生多药耐药性，经过滤和离心浓缩的病毒上清液60%细胞表达P-GP，是病毒原液基因转移率的2倍；RT-PCR表明基因转移后，肿瘤组织内mdr1 mRNA的表达量明显增加。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml，经percoll液分离后密度梯度离心，〓／ml密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心5分钟，〓／ml接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖，免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成，RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

Methods TASMCs were stimulated with LPS in the presence or absence of CCK-8 and/or PKC inhibitor chelerythrine, morphine,-OR mRNA expression was analyzed by RT-PCR 16h after stimulation.

培养大鼠TASMCs，在加入或不加入CCK-8和/或CHE、吗啡的情况下，用LPS刺激细胞16h，用半定量RT-PCR检测κ阿片受体mRNA的表达。

Methods: CD34(superscript +) cells from fetal liver were isolated with a magnetic cell sorting kit and were cultured. Cells pretreated with or without protein kinase C inhibitor chelerythrine chloride (3 μmol/L) were induced by 5 ×10^(-7)mol/L tretinoin for 24 h, and then incubated in serum-free medium. Expressions of genes in treated cells were assayed by Western blotting and RT-PCR.

采用免疫磁珠法分离人胎肝CD34细胞，培养4d后，蛋白激酶C特异抑制剂chelerythrine chloride(3μmol/L)处理24h，再加入维A酸处理24h5×10^(-7mol/L，无血清培养基培养5d，Western印迹和半定量RT-PCR法分析维A酸处理前后神经特异基因表达。

The slices of pear leaf samples infected by apple chlorotic leaf spot virus were prepared for situ RT-PCR and were detected by primed in situ labeling technique using special primer.

选用带苹果褪绿叶斑病毒的香梨叶片，制备成适合进行原位RT-PCR的石蜡切片。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。