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MTT assay arid morphological observation were used to evaluate the toxicity of rotenone on astroglia, cell proliferation and the expression of CX43 mRNA were detected by growth curve analysis and RT-PCR.

观察染毒星型胶质细胞形态，检测细胞活力，通过生长曲线分析鱼藤酮对星型胶质细胞增殖的影响以及RT-PCR检测缝隙连接蛋白(connexin43， CX43)mRNA表达。

The role of LM609 in bone marrow micrometastasis in athymic mice was detected by quantitative real-time RT-PCR.

实时荧光定量RT-PCR检测抗整合素αvβ3抗体LM609对裸鼠乳腺癌骨髓微转移模型的影响。

Methods S. epidermidis atlE gene expression level at different phases was detected by RT-PCR, and the accumulation of extracellular DNA at different phases was assessed by spectrophotometric measurements of light absorbance by DNA; DNase Ⅰ was used to study the function of extracellular DNA in biofilm formation and primary attachment. The effects of atlE gene deletion on initial adherent capacity, biofilm formation and extracellular DNA release were studied by construction of atlE deletion mutant via homologous recombination.

采用RT-PCR法检测表皮葡萄球菌atZE基因在不同时期的表达水平，用紫外分光光度计检测DNA的方法检测了相应时期的胞外DNA的释放量，并用DNA酶研究表皮葡萄球菌胞外DNA在生物膜形成和起始黏附中的作用；采用ρBT2质粒同源重组敲除的方法构建了表皮葡萄球菌1457的atlE基因突变株，研究atlE基因敲除突变对起始黏附能力、生物膜形成及胞外DNA释放能力的影响。

Methods Twenty Wistar rats were divided into 2 groups: a normal control group and a DN model group. We determined the 24 h proteinuria and other biochemic indexes, measured the ultrastructures of podocytes with electronic microscope, and detected the expression of nephrin and podocin with immunohistochemical technique and RT-PCR at the 4th and 8th weeks.

将20只大鼠随机分为正常对照组和糖尿病肾病大鼠模型组，于第4， 8周检测24h尿蛋白量等生化指标、用免疫组织化学和RT-PCR方法检测肾皮质nephrin， podocin蛋白及mRNA 表达，用透射电镜检测足细胞超微结构变化。

RT-PCR and Western blotting results showed that the transgene was expressed in the liver of the transgenic mice.

RT-PCR和Western blotting结果显示，外源基因在转基因小鼠的肝组织成功表达。

Methods The expression of HRG-1 was analyzed with RT-PCR and Northern blotting.

方法用RT-PCR和Northern blot分析HRG-1的表达。

The expression of PPARγ mRNA and protein were measured by RT-PCR and Western blotting separately.

用RT-PCR方法及Western blotting方法分别检测PPARγmRNA和蛋白的表达。

The levels of PAI-1 protein and mRNA were analyzed with Western blotting analysis and RT-PCR respectively.

采用MTT和流式细胞仪检测细胞增殖，Western blotting和RT-PCR法检测PAI-1的表达水平。

The protein and mRNA expressions of Bcl-2 and Bax were detected by Western blotting and RT-PCR, respectively.

Western blotting和RT-PCR检测各组细胞不同时点Bcl-2和Bax蛋白、mRNA的表达。

The expressing of P65 was analyzed by RT-PCR and Western blotting.

RT-PCR和Western blotting检测基因转染对P65表达的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。