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The avian influenza virus RNA was directly extracted and purified from chicken em-bryo allantoic fluid. According to published gene sequence,a pair of specific primers to the nucleoprotein gene of AIV was designed and synthesized. After that,the NP cDNA was amplified by RT-PCR .Then the complete NP gene was sequenced.

直接从鸡胚尿囊液中提取H9N2亚型禽流感病毒的RNA，并根据已发表的A型流感病毒株的核苷酸序列，设计了1对特异引物，采用RT-PCR技术成功地扩增了AIV的NP基因；将NPcDNA克隆后进行了序列测定。

The PCR products appeared to be approximate 600 bp, the expected size. The allantoic fluid of infected embryonating eggs of 6 IBV reference strains and 6 field strains were amplified by RT-PCR using three pairs of primers of 1.7, 0.2, 0.6 kb on same and different condition, 3, 5, 9 strains of IBV were amplified respectively. While 12 different sentypes of the 12 IBV strains can be divided in 6 genotypes.

用1.7、0.2、0.6 kb 3对引物对6个IBV毒株和6个分离株的含毒尿囊液在相同和不同条件下进行RT-PCR，结果3对引物分别扩增出3、5、9株IBV，同时可将不同血清型的12个IBV株分成6种基因型。

In this paper, the cDNA sequence from Ampullaria crossean stomach named Dy1300 was obtained by RT-PCR firstly and cloned into vector pMD 18-T. Its ORF named SXYN contains 882bp nucleotide encoding a 294 amino-acid protein. The percentage of similarity of SXYN with the reported cellulase EGX gene sequence from Ampullaria crossean was 89.8%.

本研究首先通过RT-PCR的方法从福寿螺胃中扩增出新的纤维素酶cDNA序列―Dy1300，并与克隆载体pMD 18-T相连接，通过对其全序列的分析，确定其开放阅读框ORF长度为882bp，命名为SXYN(GenBank， AY941794)，推定的氨基酸序列含294个氨基酸，与已报道的福寿螺纤维素酶C端基因的同源性达89.8%。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

Methods: Anergic T cell was induced by combination of B7-1 mAb and cyclosporin A in vitro, cytokine gene of anergic T cells was detected by RT-PCR.

将B7-1单抗和CsA联用在体外诱致了抗原特异性T细胞无能，采用RT-PCR法检测了无能T细胞细胞因子基因的表达。

Methods: During the April 2003 to December 2004, from the patients with different position colorectal cancer beyond Dukes C stage who were performed laparoscopic radical operation random draw 20 patients as experiment group, and from patients with different position colorectal cancer beyond Dukes C patients who were performed open radical operation 20 patients took randomly as contrast group. We draw thepatients blood about 5 ml from antecubital vein at the time before and after the operation about 30 minutes respectively, preserved the blood in deep hypothermia circumstance,cytrokeratin-20(CK-20) as the micrometastasis maker, using the RT-PCR technique,G3PDH as the intra-contrast gene, quantitatively detect the amounts of CK-20 in the blood with the gelatum imagery system and the computer analyzing software on the gelatum imagery system ,in order to compare the influence of the laparoscopic and the open operation on the micrometastasis.

在我院2003年4月—2004年12月间不同部位的临床分期在Dukes C期之内的接受了腹腔镜结直肠癌根治术结直肠癌患者中随机抽取20例作为试验组，另外在同期的接受开腹手术患者中随机抽取20例作为对照组，分别抽取术前30min和术后30min的肘前静脉血5ml，集中低温保存，以细胞角蛋白(cytekeratin-20)CK-20为微转移的标志物，应用RT-PCR技术，以G3PDH为内对照基因，应用凝胶成像系统及其计算机凝胶成像系统分析软件来定量检测手术前、后患者外周静脉血中CK-20含量，比较腹腔镜和开腹的手术方式对肿瘤微转移的影响。

RT base(4-aminodiphenylamine),as an important dyestuff intermediate and raw material for 1,4-diaminobenzene antiager, has been widely applied to rubber auxiliary, dye and pharmacy industry.

中文摘要： RT培司，化学名称4―氨基二苯胺，是一种染料中间体，也是生产对苯二胺类防老剂的重要原料，在橡胶助剂、染料、制药等行业得到广泛应用。

Methods: To detect the expression of c-myc mRNA and TA in tumor tissues ,the approximal and distal peripheral tissues, every lcm away from the tumor margin of 12 gastric and 18 colorectal carcinomas with RT-PCR and TRAP methods respectively.

用RT-PCR和TRAP方法检测12例胃癌和18例肠癌标本和切缘组织中c-myc基因mRNA和端粒酶活性的表达。

Methods 0, 2.5, 5, 10, 15 and 20 μmol/L arsenious acid-treated human hepatocellular carcinoma HepG2 cells were cultured under hypoxia for 8 hours. The cell proliferation and cell viability was assayed by WST-8 and the trypan blue dye exclusion method. The expressions of HIF-1α in human hepatocellular carcinoma HepG2 cells were checked by RT-PCR and Western Blot.

以0、0.25、0.5、1、2和4μmol/L的亚砷酸处理人肝癌细胞HepG2缺氧环境中培养8h；1μmol/L的亚砷酸处理人肝癌细胞HepG2缺氧培养0~10h，WST-8法和台盼蓝染色法分析细胞增殖和活力，采用RT-PCR和Western Blot方法检测肝癌细胞中HIF-1α的表达。

Using RT-PCR method, we cloned the gene for an G/11 xylanase from Aspergillus niger.

利用简便的RT-PCR方法克隆的木聚糖酶基因属于G／11家族。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。