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In this study, after incubating cultured human umb ilical vein endothelial cells with TNF-α for different time, we employed RT-PCR to assay IL-8 mRNA expression and immunocytochemical stain ing to detect NF-κB activation in HUVECs.

本文用TNF-α孵育培养的人脐静脉内皮细胞不同时间后，用RT-PCR法检测内皮细胞内IL-8 mRNA的表达，并用免疫细胞化学染色法检测内皮细胞内NF-κB的激活。

In the first part, it was investigated the interaction between Pb2+ and La4Ti9O24 ceramic bulk. The result shows that a significant Pb2+ diffusion into La4Ti9O24 ceramic bulk and a significant crystallization of the La2/3TiO3-type phase is clearly observed in the Pb2+ diffusion layer. The reacted zone consists of the La2/3TiO3-type phase exhibits the linear dependence of the square of the thickness, x, on the heat-treated time, t, in excellent agreement with the parabolic law x2 = kt, where k is the growth rate coefficient. The kinetic study thus indicates that the Pb2+/La4Ti9O24 interaction strictly obeys the theory of the reactive diffusion. Furthermore, the experimental k values were used to determine the associated activation energy, Ea, for the formation of La2/3TiO3-type phase using the Arrhenius plot and the following least-square equation, ln =-Ea/RT + A, where T is the annealing temperature, R is the universal gas constant, and A is a constant, resulting in Ea ~ 607 ?b 60 kJ/mol.

首先， 以巨观的方式观察PbO与La4Ti9O24陶瓷体介面的反应现象；利用X-ray绕射分析以及扫描式电子显微镜来观察不同的热处理温度以及不同的持温时间所制作的样品，研究结果发现， Pb2+离子会渗入到La4Ti9O24陶瓷内，同时在Pb2+离子所渗入的区域内会产生新的La2/3TiO3-type钙钛矿斜方晶相；藉由量测不同温度与时间之样品，其因Pb2+离子渗入而产生之La2/3TiO3-type相层厚度之关系，符合反应式扩散机制中 x2 = kt 之关系式(其中 x：扩散层厚度； k：反应速率常数； t：反应时间)；进一步将不同实验条件所得到之反应速率常数值代入Arrhenius方程式ln (k =-Ea/RT + A ，其中Ea：活化能； T：绝对温度； R：气体反应常数； A：常数，可以求得Pb2+离子与La4Ti9O24陶瓷发生反应式扩散所需之活化能为607 ± 60 kJ/mol。

Methods:Unsexed SD rats were randomly divided into shamming operation group and HIBD group.Rat models of acute brain hypoxic-ischemic were established.The expression of IL-1,IL-6 and IL-8 mRNA at days 1,3 and 7 respectively after injection was measured by rt-PCR method.

通过结扎左颈总动脉致大鼠脑急性缺血，后给8%氧气+92%氮气的混合气体致脑急性缺氧，建立大鼠脑急性缺血缺氧模型，在3个时相点采用rt-PCR方法测定假手术组、HIBD组脑组织中IL-1、IL-6和IL-8 mRNA含量变化。

Methods Serum containing achyranthes root was prepared, human mesenchymal stem cells were separated and amplified, MTT assay was used to access the effects of serum containing achyranthes root on human mesenchymal stem cells in vitro, alkaline phosphatase and osteocalcin were assayed to reflect the variations of stem cell differentiation, and the expression of osteogenic transcription factors with RT-PCR.

制备牛膝含药血清，分离与扩增人间充质干细胞，MTT法检测牛膝含药血清对体外培养的间充质干细胞增殖作用的影响，检测碱性磷酸酶与骨钙素以反映细胞分化水平的改变，RT-PCR检测成骨相关转录因子的表达。

The result indicated: antibacterial concentration of inner thallus increased progressively with time prolongation when added CCCP with antibacteria, proved that multi-resistance of E.coli in livestock and avian is mediated really by active efflux system; The multi-resistant level have positive correlation with AcrAB transcription and expression level; Quantitive RT-PCR,Western-blotting and fluorescence quantitive PCR can use to detect AcrAB.these results provide theory foundation to detect and study multi-resistance of E.coli in veterinary clinic and have a important scientific significance to develop neotype antibacterials.

结果表明，多重耐药株在有CCCP 存在时，菌体内抗菌药物的浓度随时间的延长而递增，证明畜禽大肠杆菌多重耐药性确实由主动外排系统介导；其多重耐药水平与AcrAB的转录、表达水平呈现正相关；定量RT-PCR、Western-blotting、荧光定量PCR可用于AcrAB水平的检测。这些结果对检测临床大肠杆菌的多重耐药水平、对研究兽医临床大肠杆菌多重耐药机制提供理论依据，对开发新型抗菌药物有重要的科学意义。

There is still no consensus on the expression of the two isoforms in the human prostate and their cellular locations are also controversial. It is not known to which extent each of these isozymes contributes to overall enzyme activity in different prostatic tissues. In an attempt to elucidate which isoform of the 5α-reductases is responsible for the metabolism of T in normal and diseased human prostate, we performed qualitative and semi-quantitative studies of both enzymes in normal, benign hyperplastic and adenocarcinomatous human prostate tissues by RT-PCR and Immunohistochemistry methods.

现已成功地克隆出编码Ⅰ型及Ⅱ型5α-还原酶的基因，关于这两型5α-还原酶在前列腺组织及不同类型细胞中的存在、分布情况及其对组织中睾酮代谢各自所起的作用仍存在许多争议，为此我们应用RT-PCR及免疫组化方法对国人正常、良性增生以及前列腺癌组织中两型5α-还原酶的表达进行了定性和半定量研究。

The expression sequence of LSM3 was amplified successfully from muscular tissue of giant panda Ailuropoda melanoleuca using RT-PCR technology for the first time, which was also sequenced and analyzed.

运用RT-PCR技术首次从大熊猫Ailuropoda melanoleuca的肌肉组织总RNA中成功克隆了LSM3的表达序列，并对其进行了测序及初步分析。

RNA was extracted from SIV which was purified from infected allantoic fluid, and expected band was observed via RT-PCR.

从纯化的病毒中提取RNA，进行RT-PCR，可扩增出预期的条带。

The RT-PCR technique provided a new method to directly detect the IBV from the allantoic fluid of infected embryonating eggs and tissues of infected chickens.

RT-PCR提供了直接从尿囊液和感染鸡组织中快速检测病毒的新方法。

The mice infected with allantoic fluid could arise syndromes and pathology; SIV could be isolated again and amplified by RT-PCR from the lung.

猪体回归试验，可表现出临床症状和病理变化，从肺脏中可分离到病毒并且RT-PCR也可检测到病毒相应的基因片段。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。