查询词典 rt

A full length E2 gene of classical swine fever virus was amplified and cloned by RT-PCR.

采用RT-PCR技术对猪瘟病毒E2基因进行了克隆、测序和特征分析。

RT-PCR was used to detect the expressions of COL1A1 mRNA in 20 patients with idiopathic congenital talipes equinovarus.

采用半定量RT-PCR方法检测20例单纯性马蹄内翻足患儿下肢肌肉及肌腱组织中COL1A1基因mRNA的表达，根据COL1A1基因转录调控区-1 031 bp~+30 bp及第1内含子的序列，设计8对引物， PCR扩增后，采用变性梯度凝胶电泳技术筛查突变并测序。

About recently 20 years, following advanced technology, such as CT, MRI, PET and SPECT, and intravenous rt-PA application in 3 hours after onset, people awfully gain developmental progress in its pathophysiologic stage understanding and its prevention and therapeusis early.

随着CT、MRI、PET、SPECT等影像医学的发展和rt-PA的问世，人类对急性缺血性中风的病理生理学认识及早期诊断与治疗上取得了突破性进展。

We found the UCP1 mRNA in liver of tree shrew by RT-PCR, and both the content of total RNA in liver and the expression of UCP1 were increased.

采用UCP1引物P3、P4以RT-PCR的方法在中缅树鼩肝脏中检出UCP1 mRNA的存在，并且冷驯化可使中缅树鼩肝脏总RNA的含量有所上调，使UCP1表达增加。

Using semi-quantitative RT-PCR, the differential expression profiles of eleven selected genes were confirmed in the ovaries of triploid and diploid. These genes fell in gene categories with a wide range of functions. The results indicated that triploidy affects the dynamic gene regulatory network in triploid ovary. This study established a firm basis for future investigation on characterization of crucial molecular events for normal ovarian development in shrimp.To further dissect exact gene functions for gonad development of shrimp, three differentially expressed genes between diploid and triploid ovary, PCNA (proliferating cell nuclear antigen), CAS/CSE1 (cellular apoptosis susceptibility protein/chromosome segregation 1) and SSRF (spermatogonial stem-cell renewal factor) were characterized on certain aspects.

利用抑制性消减杂交技术，建立了对虾二倍体和三倍体卵巢间的2个消减文库；在正向消减文库(以三倍体卵巢作为实验组，二倍体卵巢作为驱动组)中，鉴定到54个基因；在反向消减文库(以二倍体卵巢为实验组，三倍体卵巢为驱动组)中，鉴定到16个基因；选取11个差异表达的基因，利用半定量RT-PCR的方法对其在二倍体和三倍体卵巢间的表达进行了检测，均能很好地与消减结果相吻合；这些差异基因编码多种功能的蛋白，分析表明染色体的三倍化使三倍体卵巢中的基因调控网络受到了影响；为深入揭示维持卵巢正常发育的关键分子调控事件奠定了基础。

We detected the five varieties citrus mother plants and STG plants with RT-PCR, as a result, all of the mother plants could amplified Tristeza bands, but the highest virus-free rate of grafting plants only was 61.1%, and the results may prove that not all shoot-tip grafting can remove the CTV completely.

对瓯柑、稻叶蜜柑、市文蜜柑、翡翠柚、处红柚等6个品种的母株和茎尖嫁接苗进行了RT-PCR检测，结果所有样品的母株均扩增出了衰退病特异性条带，而STG嫁接成活苗的无毒率最高仅为61.1％，证明茎尖嫁接方法并不能完全脱除衰退病病原。

A multiplex RT-PCR was successfully established to simultaneously detect Citrus Huanglongbing pathogen ( Candidatus Liberibacter asiaticus, HLB), Citrus exocortis viroid and Citrus tristeza viru s. Using three sets of specific primers designed according to the converted sequences of these three

建立了一种同时检测柑橘黄龙病病原类细菌( Candidatus L iberibacter asiaticus， HLB)、柑橘裂皮类病毒( Citrus exocortis viroid ， CEVd)、柑橘衰退病病毒( Citrus tristeza virus ， CTV)的多重RT-PCR技术体系。

Methods:The whole mature protein coding sequence of three truncated dystrophin gene was amplified by RT-PCR method applied to human muscle cDNA. The fragment was inserted into prokaryotic expression vector pET28a plasmid.

以人肌肉组织cDNA为模板，采用RT-PCR扩增三个截短的肌营养不良蛋白cDNA，分别克隆入原核表达载体pET28a中，经限制性内切酶双酶切及DNA序列分析鉴定目的基因。

According to 5'end constant nucleotide sequences of all trypanosome mRNA,we designed and synthesized RT-PCR downstream primer 5'-GAACAGTTTCTGTACTATATTG-3'.

根据所有锥虫mRNA在5'端的保守序列，设计合成了RT－PCR下游引物5'—GAACAGTTTCTGTACTATATTG—3'。

The CCND2 expression cell proliferation, cell cycle and cell apopotosis of the transfected LP-1 cells were studied by RT-PCR, trypanosome staining, flow cytometry and annexin V assay.

设计、合成CCND2短发夹RNA双链DNA模板，构建表达质粒pGenesil-2／CCND2，转染LP-1细胞，以RT—PCR检测对CCND2表达的抑制作用，以锥虫蓝染色计数和MTI检测细胞增殖，以Annexin V检测细胞凋亡，流式细胞术检测细胞周期。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。