查询词典 rt

A549 cell line was stimulated with LPS (10 μg/mL) and then treated with Rs504393 (10 μg/mL) for 6 hours. ALI model was established with intranasal administration of LPS (5 mg/kg) in C57BL/6J mice. RS504393 (5 mg/kg) was administered 30 min before LPS dripped nasally. IL-8, IL-1β, plasminogen activator inhibitor-1, monocyte chemoattractant protein-2, and the expressions of CCR1 and CCR2b were studied by using Realtime-RT-PCR, ELISA and cyto-flowmetry.

体外实验选用A549细胞系，应用LPS(10μg/mL)刺激合并RS504393(10μg/mL)治疗6 h后，流式细胞仪技术检测CCR1和CCR2的表达，ELISA法检测细胞培养上清液内白介素-8的浓度；体内实验选用C57BL/6J小鼠，腹腔注射RS504393(5 mg/kg)预处理30 min后经鼻滴入JPS(5 mg/kg)，LPS刺激4 h后收集BALF和肺脏标本，计数BALF内细胞总数，应用BCA法检测BALF内蛋白浓度，Realtime-RT PCR和ELISA法检测BALF和肺脏IL-1β、凝血酶原激活物抑制物-1和单核细胞趋化蛋白-2的表达。

During the breeding process of Penacus vannamei,the PCR and RT-PCR techniques are applied to detect three types of virus,that is,WSSV,IHHNV,TSV,to protect the parental shrimp,egg,nauplius,parental shrimp feed,artemia and water from being infected.The purpose of this work is to breed the healthy Penacus vannamei,and to offer a feasible measure to keep a sustainable development for Penacus vannamei breeding.

应用PCR和RT-PCR技术对WSSV、IHHNV和TSV这3种对虾病毒在凡纳滨对虾育苗过程中各个环节进行了检测，防止亲虾、卵、无节幼体、仔虾、亲虾饵料、丰年虫、水体等诸环节受到以上3种病毒携带或感染，保证培育出的凡纳滨对虾仔虾是高健康的虾苗，为凡纳滨对虾健康养殖的可持续发展提供一条可行的途径。

By using RT-PCR method, partial sequences of ppo genes were amplified from cDNA of Nelumbo nucifera's young leaves. After cloning and sequencing, 7 sequences ranging from 978bp to 1057bp were obtained, and they encoded 326 to 352 amino acids.

用RT-PCR方法，从中国莲幼叶RNA中成功扩增出ppo的部分序列，经克隆测序，得到长度在978~1057bP的序列共7个，编码326~352个氨基酸。

In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia cells by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during early HCMV infection.

本研究采用RT-PCR和Western Blotting技术，检测正常培养和感染HCMV的神经胶质瘤（U251）细胞NGF的表达，探讨人巨细胞病毒对神经胶质瘤细胞内源性神经生长因子表达的影响。

The level of NIS gene expression was assessed by RT-PCR.

采用RT-PCR方法检测细胞中NIS的mRNA表达情况。

We detected the expression of OCY-66, OCY-189 and OCY-142 in 28 ovarian cancers and 30 normal ovarian tissues by RT-PCR, and studied the expression of OCY-142 in these ovarian cancers and normal ovarian tissues by nonradioactive hybridization in situ.

采用RT-PCR和原位杂交的方法研究卵巢癌相关肿瘤抗原新基因在卵巢癌组织和正常卵巢组织中的表达，分析其与卵巢癌临床指标的关系。

The transform of phenotype of VSMC: The mRNA expressions of SM22α-a notate gene of contractile VSM-were all detected on normal aortas,but decreased normally when the aortas were destroyed and cultured in vitro for 10 days,especially in the serum groups,whose SM22αmRNA could not be detected by RT-PCR.Here,BQ123 can increased their expressions.This demonstrated that serum culture and the secretion of ET-1 can produce the transform of phenotype from contractile to synthesize type.

血管平滑肌表型的转化：RT-PCR检测发现，血管平滑肌收缩表型标志基因SM22α在正常血管平滑肌中都有表达，但在内皮损伤后体外培养10天的血管表达明显减弱，其中血清培养组血管基本不表达，加内皮素受体阻断剂BQ123后，各组SM22α的表达均有所上调，这表明内皮素分泌和血清培养促进了血管平滑肌的表型由收缩型向分泌型的转化。

PE-RT is referred to as the Polyethylene Raised Temperature, it is by the ethylene monomer and 1 - octene copolymer from monomers is specifically designed for the heating system of medium-density ethylene - octene copolymer, which has a narrow molecular weight distribution , octene uniformly distributed in the polymer main chain on the special molecular structure, it retained the original PE performance and processing properties of the health advantages, but also strengthen the durability of a new type of high-temperature resin pipe.

PE-RT是Polyethylene Raised Temperature的简称，它是由乙烯单体和1-辛烯单体共聚而成的，是专门为采暖系统而设计的中密度乙烯－辛烯共聚物，其具有分子量分布狭窄，辛烯均匀分布在聚合物主链上的特殊分子结构，它既保留PE原有的卫生性能及加工性能等优点，又强化了高温耐久性的一种新型管材专用料。

One-step RT-PCR could be used in detection of human rabies. It was important for forensic identification.

一步法RT-PCR可用于人狂犬病毒的检测，在法医学鉴定中具有重要意义。

Pig ; cationic amino acid transporter ; real-time RT-PCR ; ontogenetic regulation

猪； 碱性氨基酸转运载体；实时荧光定量RT-PCR ；发育规律

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。