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Methods RT-PCR and RACE methods were applied to clone the full-length panallergen gene from Caryota mitis pollen. The sequence of the insert was analyzed.

利用RT-PCR结合RACE技术克隆短穗鱼尾葵花粉中泛变应原profilin的全长基因，并进行序列分析。

The ORF of profilin of Caryota mitis pollen was amplified by RT-PCR using specific primers and cloned into the expression vector pET-28a. The recombinant Caryota mitis pollen profilin was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified by affinity chromatography using Ni(superscript 2+) coupled sepharose.

然后设计带有酶切位点的特异性引物，采用RT-PCR获得整个短穗鱼尾葵花粉profilin的开放阅读框，将其与pET28a载体连接并转化大肠杆菌BL21(DE3)进行诱导表达，通过Ni(上标 2+)亲和层析柱对重组蛋白进行纯化，采用Western-blot检测其IgE结合活性。

The study in this paper, a Pollen-specific Lysine-rich Protein cDNA, from tomato by RT-PCR was obtained. A plant expression plasmid pBIUB-TSB was constructed and lysgene was under the control of maize ubiquitin promoter which is the highest efficient monocotyledon promoter.

本实验通过RT-PCR从番茄花粉中获得高效特异表达的高赖氨酸基因TSB的cDNA序列，构建其受控于单子叶植物Ubil强启动子下的高效表达质粒pBIUB-TSB。

Expression of peripheral monocyte MMP-9 was measured by RT-PCR and Western blot.

分离大鼠外周血单核细胞，用RT-PCR和Western blot检测MMP-9表达的变化。

The results of RT-PCR showed that the gene was expressed at all examined developmental stages and tissues of B. mori.

RT-PCR实验表明该基因在本实验所调查的家蚕不同发育时期和组织中都有表达。

The cDNA encoding AChE from susceptible and propoxur-resistant strains of Musca domestica had been cloned using RT-PCR.

2利用RT-PCR技术克隆了家蝇敏感和残杀威抗性品系的乙酰胆碱酯酶基因。

The cDNA sequence encoding ubiquitin from Musca domestica was cloned by RT-PCR and sequenced.

本研究根据GenBank已登录的真核生物泛素编码框的氨基酸序列，设计一对简并引物，RT-PCR克隆了家蝇Musca domestica泛素基因的编码区cDNA序列，并进行了测序。

TTC staining,RT-PCR and Western blot analysis were used to study the effects of muscone on infracted volume,EAAClmRNA and protein levels.

运用Western blot法和TTC染色观察缺血区EAAC1表达和梗塞体积；采用RT-PCR和Western blot法，测定海马EAAC1 mRNA和蛋白在缺血1h、6h、24h的变化。

A pair of primers for amplification of σC gene was designed and synthesized according to the published Muscovy duck reovirus S4 gene sequence. The specific RT-PCR product was success-fully obtained and cloned from the Muscovy duck reovirus ZJ99 strain.

参考已发表的番鸭呼肠孤病毒的S4序列设计了1对扩增σC蛋白基因的引物，对mDRV ZJ99株的RNA抽提物进行RT-PCR扩增，获得了特异性的扩增片段。

Methods:The dynamic cytotoxicity of ADR or / and NaBu was evaluated by MTT assay. The telomerase activity was analyzed by TRAP and silver staining. The expression of hTERT was assessed by RT-PCR.

MTT法检测NaBu、ADR以及两药联用的药效动力学特征；TRAP-银染法检测端粒酶活性；RT-PCR法检测hTERT mRNA表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。