查询词典 rt

Inconclusion, to hybridize at 60℃ for 18h was the best condition of the low-density c DNA microarray . The reproducibility and reliability was good. The specificity was superior to RT- PCR and the sensitivity inferior

由此可见，60℃杂交18h 为该自制低密c DNA 芯片的最佳杂交条件，该芯片的重复性与可靠性较好，与RT- PCR 技术相比，特异性很好，灵敏度略差。

Although there was a long delay between diagnosis and the start of RT, DLCO was not a variable index without treatment.

虽然有之间诊断和 RT 开始的长延迟 DLCO 不是没有接受治疗下的一个变量索引。

Real-time quantitative RT-PCR results indicated that BmUGT004965 was expressed only in silk gland and fat body among 9 tissues detected on day 3 of the fifth instar larvae, and the expression level in silk gland was much higher than that in fat body, which is consistent with the microarray analyzing results. The specific expression pattern may relate to the special function of this gene.

实时定量RT-PCR检测表明，在5龄第3天家蚕幼虫的9种组织中，BmUGT004965基因只在丝腺和脂肪体中有表达，且丝腺中的表达丰度明显高于脂肪体，这与基因芯片的结果一致，推测这种特异的表达方式可能与其特异的功能有关。

In ChibiOS/RT the counting semaphores are mainly meant as a synchronization mechanism between interrupt handlers and high level code running at thread level.

在ChibiOS/RT中计数信号量多数情况下表示的是在中断服务与线程层面上的高级代码之间的同步机制。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段，应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段；在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法；检测方法的特异性试验结果表明，该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应；检测方法的敏感性试验结果表明，通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg；对20份临床样品检测中，有10份为AIV阳性，其中有5份为H5亚型病毒阳性。

Methods The Diaphragmatic muscle, latissimus dorsi and gastrocnemius muscles of the C57BL/10ScSn-Dmd/J and C57BL/6J mice were collected at the age of 2, 3, and 4 weeks, respectively. Each group selected 3 mice for experiments. Dmd gene were amplified and analyzed by RT-PCR.

选取2、3和4周龄C57BL/10ScSn-Dmd上标(上标 mdx/J模型小鼠及C57BL/6J小鼠各3只，分别采集膈肌、背阔肌和腓肠肌的肌肉组织，利用RT-PCR方法扩增分析Dmd基因的结构特点；采用Western bolt方法分析β-dystroglycan的表达差异。

Semiquantity RT-PCR method was used to detect the COLlal and COL3al in the ligamentum teres at mRNA level.

采用半定量RT-PCR及Western-Blot法检测COL1a1、COL3a1在mRNA及蛋白水平的表达。

At 0, 4, 8, 16, 24 h after operation, the expressions of SR-A protein and mRNA in liver tissue were assayed by western blot and RT-PCR respectively; the levels of plasma endotoxin and interleukin-6 (IL-6) were measured using limulus test and ELISA.

于术后0、4、8、16、24 h分别采用Western blot和RT-PCR检测肝组织中SR-A蛋白和基因表达水平；以鲎试验和ELISA测定血浆内毒素和白介素-6(IL-6)；光镜观察肝组织病理变化并测定血浆ALT、TB含量。

In this study, we found out that linalool, a small-molecule compound, could inhibit the growth of various human lymphocyte leukemia cell lines significantly. Meanwhile, it showed little toxicity to normal hematopoietic cells. To determine effects of linalool on the growth of a serious of human leukemia cell lines and normal hematopoietic cells and to explore its possible mechanisms, we employed various technologies, including MTT assay, Flow cytometry, Human Cancer PathwayFinder II Gene Array, Western Blot, RT-PCR and so on.

我们在筛选抗肿瘤小分子化合物时，发现天然小分子化合物芳樟醇能明显抑制多种人淋巴细胞白血病细胞增殖，而对正常人骨髓造血细胞及外周血细胞的增殖没有明显的影响，为此我们用MTT技术、流式细胞术、肿瘤相关信号通路分析技术以及Western blot、RT-PCR等手段和方法探讨了芳樟醇对不同类型白血病细胞系及相应正常造血细胞中的影响情况，及其诱导白血病细胞凋亡的可能分子机制。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。