查询词典 rt

Methods SLN of 40 patients with breast cancer were performed histological analysis by hematoxylin and eosin staining, and the expression level of CK 19 in these nodes were determined by quantitative RT-PCR.

目的 探讨定量RT-PCR法检测细胞角蛋白(CK19)在乳腺癌前哨淋巴结中的表达，提高前哨淋巴结活检中微转移的检出率。

In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期，高度表达的合子转录本仍在所有的胚盘细胞中，但至90%下包期时，pou2的表达向胚胎背部的正中线汇聚，集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时，pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时，转录本消失，这与RT-PCR结果高度一致。

Preadipocytes from epiploon of newborn calf were cultured by monolayer adherent method, and total RNA of the preadipocytes was extracted on day 4,6,8,10,12,14 and 16 post-culture, respec-tively. The abundance of ADPN mRNA and HSL mRNA was detected by real-time fluorescent quanti-tative RT-PCR during differentiation period from preadipocyte to adipocyte.

选取犊牛大网膜脂肪组织，进行牛前脂肪细胞的单层贴壁培养，在细胞接种后第4、6、8、10、12、14、16d分别提取细胞总RNA，采用实时荧光定量RT-PCR方法，检测了体外培养的犊牛前脂肪细胞不同时期ADPN基因mRNA和HSL基因mRNA的表达情况。

Methods: Three aspects were studied in the epithelization of skin of PAI-3 with the techniques of Immunohistochemistry,Immunocytochemistry,RT-PCR and the analysis of PAI-3 in cornified envelope.

采用免疫组织化学(Immunohistochemistry，IHC)、(Immunocytochemistry，ICC)和逆转录-聚合酶链反应(reverse transcription PCR，RT-PCR )技术及KC角质壳的提取，从三个方面研究了PAI-3 在人皮肤上皮化中的表达及作用。

Therefore the established one-step RT-PCR technique provided a sensitive, specific and reliable method for diagnosis and epizootic study of the BVDV.

初步结果表明所建立的一步法RT-PCR技术可用于牛病毒性腹泻／粘膜病的检测及流行病学调查。

Key words: avian influenza virus; one-step RT-PCR; diagnoses expeditiousness

禽流感病毒；一步法RT-PCR；快速诊断

Primary atrial tumor has obvious characteristic on RT-3DE: All the atrial tumor display the same size of stereoimages as the tumor themselves. There is a clear boundary between the stereoimage of the myxoma and the atrial wall and there exists a short pedicle connecting the atrial myxoma to the atrial wall or the interatrial septum. The body of the atrial myxoma moves to and fro through the atrium and the atrioventricular valve with the contraction and relaxation of the heart with a fixative pedicle. Atrial myxoma leads to relative atrioventricular valve incompetence during systolic phase and relative atrioventricular valve stenosis during diastolic phase, but doesn't result in pathological changes of atrioventricular valve which restore to normal after exsection of the atrial myxoma. Malignant atrial tumor closely and extensively contact with atrial wall without obvious boundary, pedicle and movement with the contraction and relaxation of the heart.

所有心房肿瘤RT-3DE均实时显示与肿瘤大小相同的立体形态图像；心房黏液瘤立体形态图像与心房壁界限明显，都通过一短蒂与房间隔或心房壁相连，瘤体以蒂为固定点随心脏收缩、舒张在心房与房室瓣口之间往返运动；心房黏液瘤收缩期瘤体导致房室瓣相对关闭不全，舒张期瘤体导致房室瓣相对狭窄，但瘤体不引起房室瓣器质性病变，瘤体摘除后房室瓣功能即恢复正常；恶性心房肺瘤立体形态图像与心房壁关系密切，界限不明显，接触范围广泛，无蒂，瘤体不随心脏收缩、舒张运动。

The fragment of amplification was 247bp in size using the external primers.After optimization of the ratio of the internal to the external primers,concentration of dNTP and Mg2 +,temperature and time of reaction,a RT-LAMP method for specific detection of CDV was established successfully.

通过对反应条件中内、外引物的比例、dNTP浓度、Mg2+浓度、反应温度和时间等条件逐一优化，成功建立了犬瘟热病毒的RT-LAMP检测方法。

According to experimental results and etiopathogenisis and pathology of osteoporosis , and analyzing review investigation on hepcidin、associations of bone metabolism and iron metabolism and relations of ferri ion and calcium ion,we can make conclusions as follows: 1、We applied single manmade intervention approach to induce osteoporosis model in this study, there was no other influential factor. In the termination of test , rats bone density and bone histological anatomy indicated that osteoporosis model was creditable,meanwhile hepatic functional enzyme and hepatic microtome section demonstrated that hepatic function had noci-influence induced by medicinal herbs resource. Accordingly, we could believe that hepatic hepcidin gene expression and serum hepcidin contents can represent internal iron metabolism changes at selected test time. 2、In this investigation, different findings between test group and control group indicated that:according to changes of hepcidin gene expression measured by RT-PCR and changes of serum hepcidin contents determined by ELISA kit , there were correlations between hepcidin and rat osteoporosis model induced by retinoic acid. 3、Under the condition of this investigation,there was a interactive hypothesis as follows: Hepcidin iron metabolism←→ferriion←→calcium ion←→osteoporosis.

根据本次实验结果，对骨质疏松的病因病理的认识及铁代谢与铁调素、骨代谢与铁代谢关系、铁离子与钙离子的关系系列研究的回顾，经分析得出如下结论：1、本研究骨质疏松模型采用单一人工干预方法，没有其他影响因素；实验结束时大鼠骨密度、骨组织学检查表明骨质疏松形成，同时肝脏功能酶和HE切片显示肝脏功能未受药源性产生明显损伤性影响；因此，同期不同时间组大鼠肝脏铁调素基因表达、血清铁调素含量可以代表体内铁代谢不同时间的变化。2、在本研究中，模型组与对照组研究结果的差异表明：铁调素基因RT-PCR变化、血清铁调素ELISA测定含量变化与维甲酸制作的大鼠骨质疏松模型形成有实验相关性。3、在本研究平台下，形成了一个互相影响的假设图：铁调素←→铁代谢←→铁离子←→钙离子骨质疏松。

Methods The expression of fez gene was detected by RT-PCR in thirty-six cases of esophageal cancer tissues, matched paracancer tissues and tissues far away from tumor.

采用RT-PCR检测36例食管鳞状细胞癌癌组织、相应的癌旁组织和远癌组织中fez mRNA的表达情况。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。