查询词典 rottlerin

The present study also demonstrated that rottlerin significant prevented the PDT with aloe-emodin-induced the phosphorylation and fragmentation of Bcl-2 in H460 cells.

在本研究中，rottlerin也显著的抑制芦荟大黄素配合光照引起H460细胞之Bcl-2磷酸化及断片的现象。

Furthermore, inhibition of TG2 by rottlerin or by the siRNA led to accumulation of green fluorescent protein-LC3-II in autophagosomes in pancreatic cancer cells transfected with GFP-LC3 (GFP-ATG8) expression vector.

此外，抑制TG2的rottlerin或小分子干扰核糖核酸导致积累的绿色荧光蛋白- LC3 - II在autophagosomes在胰腺癌细胞转染绿色荧光蛋白LC3 （绿色荧光蛋白ATG8 ）表达载体。

Finally, we used GF109203X, Rottlerin, PKC-delta antisense RNA and PKC-epsilon siRNA to inhibit PKC activation or expression, and then observed their effects on TRAIL-induced apoptosis.

当以Rottlerin处理细胞时，均会增加细胞对於TRAIL的感受性。除了H1299细胞，p53能透过藉由Rottlerin抑制PKC-delta的活性，进而增加细胞对TRAIL诱导细胞凋亡的情形。

In this study, rottlerin, a PKCδ-selective inhibitor, was used to examine the mechanisms of PDT with aloe-emodin-induced H460 cell anoikis.

本研究也以PKCδ选择性抑制剂rottlerin评估芦荟大黄素配合光动力疗法引发H460细胞之失巢凋亡的真正机制。

Inhibition of PKCdelta by rottlerin or knockdown of TG2 protein by a TG2-specific siRNA resulted in a marked increase in autophagy shown by presence of autophagic vacuoles in the cytoplasm, formation of the acidic vesicular organelles, membrane association of microtubule-associated protein 1 light chain 3 (LC3) with autophagosomes, and a marked induction of LC3-II protein, important hallmarks of autophagy, and by electron microscopy.

抑制PKCdelta的rottlerin或击倒的TG2蛋白质的TG2特定的siRNA导致显着增加，表明自噬存在自泡在细胞质，形成酸性囊泡细胞，膜协会微管相关蛋白1轻链3（ LC3 ）与autophagosomes ，并显着诱导LC3 -Ⅱ蛋白的重要标志吞噬，并通过电子显微镜。

The mechanisms by which rottlerin inhibited PDT with aloe-emodin-induced H460 cell anoikis probably involved the changes in mitochondrial functions, the expression of cytoskeleton regulatory proteins and the translocation of PKCα,δ and ε.

我们证实rottlerin对於芦荟大黄素配合光动力疗法引发H460细胞失巢凋亡的抑制应与粒线体功能、PKC家族成员的蛋白分布及细胞骨架调控蛋白的表现有关。

Nucleotide-induced eNOS phosphorylation and activity were inhibited by BAPTA-AM (an intracellular free calcium chelator), rottlerin, and protein kinase C siRNA.

BAPTA-AM（一种细胞内游离钙螯合剂），rottlerin和蛋白激酶C siRNA抑制核苷酸介导的内皮型一氧化氮合酶磷酸化和活化。

Our results indicate that 1 NSC606985, at nanomolar level, can effectively induce apoptosis in AML cells NB4 and U937 and significantly inhibit the proliferation without cell death in breakpoint cluster region–Abelson murine leukemia kinase-carrying leukemic K562 cells; 2 At such low concentrations, this agent also significantly inhibits the clonogenic activity of hematopoietic progenitors from patients with AML; 3 For apoptosis induction, NSC606985 rapidly induces the proteolytic activation of protein kinase Cδ with loss of mitochondrial transmembrane potential and caspase-3 activation; 4 Co-treatment with rottlerin, a PKCδ-specific inhibitor, completely blocks NSC606985-induced mitochondrial m loss and caspase-3 activation, while the

结果显示：(1)纳摩尔浓度的NSC606985即可有效诱导AML细胞系NB4和U937细胞凋亡并显著抑制含有bcr-abl融合蛋白激酶的K562细胞的增殖；(2)低浓度的NSC606985也显著抑制来自AML病人骨髓的新鲜白血病细胞的克隆形成能力；(3)除了迅速诱导线粒体跨膜电位的崩塌及Caspase-3的活化外，NSC60698也导致蛋白激酶Cδ的水解激活；(4)PKCδ特异的抑制剂rottlerin能够完全抑制NSC606985诱导的线粒体跨膜电位的崩塌和Caspase-3的活化，而Caspase-3特异的抑制剂z-DEVD-fmk仅能部分削弱PKCδ的活化及细胞的凋亡；(5)以移植PML-RARα转基因小鼠产生的白血病细胞建立的模型研究了该化合物对白血病可能的治疗作用。

Restoration of ODC expression in the HL60-ODC cells resulted in rescue of rottlerin-induced apoptosis of HL60 cells. Simultaneously, phosphothreonine-ODC of rottlerin-treated HL60-ODC cells was highly elevated comparing with HL60-pcDNA3 cells. Taken together, we suggested that rottlerin, a PKCd inhibitor, might decrease phosphothreonine-ODC to reduce both protein stability and enzyme activity of ornithine decarbooxylase and finally resulted in cell apoptosis.

为了侦测鸟胺酸去羧化是否能够负调节rottlerin所引发的细胞凋亡，我们将具有鸟胺酸去羧化cDNA载体稳定转染至HL60细胞内，此细胞称为HL60-ODC细胞，另转染载体的细胞称为HL60-pcDNA3细胞；实验发现HL60-ODC细胞中的鸟胺酸去羧化蛋白为HL60-pcDNA3细胞与HL60细胞表现之2.5倍，同时HL60-ODC细胞提升鸟胺酸去羧化蛋白表现，能够减少rottlerin造成之细胞凋亡现象，并且此蛋白在HL60-ODC细胞中酥胺酸磷酸化较HL60-pcDNA3细胞表现高。

Our data are showed that apoptosis parameters including DNA fragmentation, nuclear morphological change and sub-G1 fraction, appeared after 24 h by 5 μM rottlerin treatment. Furthermore, rottlerin caused a decrease in 72% of ODC protein expression and an increase in threonine, rather than serine and tyrosine dephosphorylation on ODC enzyme compared with the control after incubation for 10 h. To determine whether ODC could down-regulate rottlerin-induced apoptosis, HL60 cells were stably transfected with ODC cDNA. HL60-ODC cells exhibited a 2.5-fold increase in ODC protein compared with vector only (HL60-pcDNA3) or HL60 cells.

在我们筛选天然化合物实验中，发现rottlerin抑制鸟胺酸去羧化活性最具成效，之前的研究人员已知蛋白激Cd调节鸟胺酸去羧化的活性，因此在人类白血病HL60细胞处理rottlerin的实验中发现加入rottlerin二十四小时后，细胞进行计划性死亡，并可侦测到DNA片段、细胞核浓缩及sub-G1提增；而细胞处理药物十小时后与控制组细胞比较，观察到鸟胺酸去羧化的蛋白表现与活性下降50﹪以上；除此以外，测定鸟胺酸去羧化去磷酸化的情况，发现於酥胺酸位置有显著差异，而丝胺酸和酪胺酸位置并无差异。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新