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After that, the comparison of the fertilizable competences of ICSI and IVF on cryopreservated oocytes was studied in this article, in order to establish an optimized method to improve the fertilizable competences of oocytes up to the level of embryos.1 Effects of the reducing injury methods on IVF of cryopreservated oocytesThe result of the three methods, choosing the right time oocytes, using minimum size sample vitrification and culturing after thawing, showed that these ways could partly maintained the fertilizable competences of cryopreservated bovine oocytes.

减轻损伤方案对冷冻卵母细胞IVF的影响使用选择合适时期的卵母细胞进行冷冻；采用最小样本量玻璃化冷冻方法进行冷冻；在解冻后先孵育再进行体外受精等三种方案能够部分维持或恢复冷冻牛卵母细胞IVF受精能力。

Breed helps advance somebody's career: Can sow breed, but 3 months left and right sides can maintain to lose gemmiparous power namely only below normal temperature after the seed is collected, and fruit hard surface is solid, water penetration is poor, gemmiparous very not orderly, after appropriate uses wet sand bed to accumulate vernalization, the seed with seasonable budding winkle, with nutrient bag grow seedlings, grow 60-70 till Xiaomiao centimeter when, choice monsoon field planting at earthy and loose in fecund loam.

繁育栽培：可播种繁殖，但种子采后在常温下只能保持3个月左右即失去发芽力，且果壳坚硬，透水性差，发芽很不整洁，宜采用湿沙层积催芽后，及时挑出萌芽的种子，用营养袋育苗，直到小苗长到60-70厘米时，选择雨季定植于土质疏松肥沃的壤土中。

Forty healthy pigment rabbits without oculopathy were divided into 8 groups, 1 hour, 3 hours, 1 day, 3, 7, 14, 28 days after retinal detachment and normal control, 5 rabbits in each group. Right eye of each rabbit was treated with infusing sodium hyaluronate under retinal neuroepithelium layer. The eyeballs were enucleated at different times after RD. The morphologic changes of the retina were observed by electron microscopes. Terminal deoxynucleotidyl transferasemediated dUTP nick end labeling technique was used to assess apoptosis.

健康成年无眼部疾病青紫兰兔40只，采用计算机随机数字表法分为RD后1， 3h；1， 3， 7， 14， 28d组及正常对照组，共8组，每组5只，选取右眼为致伤眼，视网膜下注射透明质酸钠建立RD模型；分别于建立模型后按时获取兔眼标本，以TUNEL法观察视网膜神经感觉层细胞的凋亡情况。

If there is joint on the back, in the lower right or fill a 20% discount after it has been causing wrinkles without distortion; vacuum packaging vacuum after the contents of the packin mouldedround radiofrequent nonanatomical tridimension on the surface, shape, surface roughness, would create a bar code reading of deformation cannot, therefore, be appropriate for the vacuum packing inner bag direct business card printing and membership card to make a bar code, you can increase the packin business card printing outside and membership card to make a bar code, and other graphics and text.

如背面中央有接缝，则印在右下方或印在填充后不起皱折、不变形处；真空包装抽真空后包装袋紧贴在内容物表面，呈不规则立体形状，表面不平整，会造成条形码变形不能识读，因此真空包装内袋不宜直接制卡和会员卡制作条形码，可增加一个外包装袋制卡和会员卡制作条形码及其他图文。

objective to discuss the nursing methods of acute postrenal renal calculus obstruction through ureteroscope.methods the preoperative and postorerative aborative nursing in 22 cases of patients with ureter calculus who were treated by emergency ureteroscope were analyzed retrospectively.results all patients became well and renal function was improved quickly after operation,after the timely treatment and nursing,none patients occured the serious complication.conclusion timely and right preoperative preparation,mental nursing and postoperative nursing were essential for the success of operation.

目的 讨论经尿道输尿管镜治疗急性肾后结石梗阻的护理方法。方法对22名患者进行急诊经尿道输尿管镜，治疗上尿路结石的术前、术后精心护理的回顾分析。结果所有患者顺利接受手术，术后患者全身状况、肾功能快速恢复，并发症得到及时治疗和护理，无一例发生严重并发症。结论及时准确的术前准备、心理护理、术后护理、是保证手术成功的重要保障。

Results: this a 19 years old patient with moderate differentiated adenocarcinoma of right seminal vesicle. the tumor recurred twice locally after local excision of the tumor combined with radiotherapy and prostatovesiculectomy. he was eventually cured by radical excision of bladder with indiana pouch. the patient is still alive without recurrence more than 10 years after the last operation.

结果：1例发病年龄19岁的右精囊中分化腺癌病人，先后经过肿瘤局部切除术加放疗和前列腺精囊切除术，两次治疗后肿瘤均局部复发，最后又行膀胱全切，indiana膀胱术后治愈，病人从最后一次手术至今已接受随访10年以上，至今无复发仍存活。

There were no statistically differences between the 8th~ 14thday, the 15th - 21th day post-BC and the control group. During the rt~24tkday post-BC , pycnosis degeneration or necrosis neurons of the locus cerebral cortex, dorsal hippocampus, dentate fornix were significantly increased, then decreased gradually, but recovered to normal by the 24thday after BC, and especially in parietal cortex and piriform cortex the necrosis neurons were significantly increased than temporal cortex , and there were no statistically difference berween the left and the right side. Pycnosis degeneration or necrosis neurons in the brainstem reticular formation were markedly increased in the 4thday after BC, and there were no statistically difference among the other groups and the control group.

与对照组相比，BC后在大脑皮质、背侧海马和齿状回部位，固缩变性和不完全坏死细胞数先显著增加(P＜0.05)，然后逐渐减少，至24d基本恢复正常；顶叶、梨状皮质比颞叶皮质变性坏死细胞多，差异有高度显著性(P＜0.01)；大脑左侧比右侧变性坏死细胞稍少但差异无显著性；在BC后4d，脑桥核、斜方体核平面的脑干网状结构中固缩变性和不完全坏死细胞数明显增多(P＜0.05)，其它组间无显著性差异。

Every indicatrion has statistics difference after six mouthstreatment of Two rattan mixture which include body function, activity index number of pathogenetic condition, total swelling arthrosis, total self-assessment, rachialgia, rachiodynia rhachitis, activity of thoracic skeleton, schober, distance between fingers and ground, left flection of antisternum, right flection of antisternum, antisternum hypsokinesis, distance between head and wall, ESR, C reactive protein: every indicatrion has statistics difference after the treatment of Sulfasalazine except the indicatrion of distance between head and wall.

二藤合剂组治疗6个月后其各项观察指标(包括躯体功能、病情活动指数、肿胀关节总数、总体自我评价、脊柱痛、脊柱炎症，胸廓活动度、Schober、指地距、脊椎左屈、脊椎右屈、脊椎后仰、枕墙距、血沉、C反应蛋白)与治疗前相比，均有统计学差异(P＜0.05)；柳氮磺吡啶组除枕墙距外，其余观察指标亦均有统计学差异(P＜0.05)。

Postoperative right-sided obstruction occurs more frequently after ASO repair of Taussig-Bing heart than after TGA arterial switching, leading to higher reintervention rate.

Taussig-Bing 心脏畸形行心脏转位术（ASO比大动脉转位行心脏转位术后更可能发生术后右室流出道梗阻，导致更高的再介入率。

Through investing Internet company, after the rapid development that witnessed Netease, Tecent, the first round of bubble of Chinese Internet was faced with burst 2000, right now Liu Xiaosong is planning to establish his an Internet company, fortunately, he chose to enter wireless Internet market, sheered the website after bubble is undone to shut spring tide.

通过投资互联网公司，目睹了网易、腾讯的快速发展后，2000年中国互联网的第一轮泡沫面临着破裂，此时的刘晓松正计划创建自己的一家互联网公司，幸运的是，他选择了进入无线互联网市场，躲开了泡沫破灭后的网站关闭大潮。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。