查询词典 ribozyme

Objective:To design the antisense RNA gene and hammerhead ribozyme gene according to the hTR sequence in the intrahepatic bile duct cancer cell,and insert them into the eukaryon expression vector pTriEx-4 separately.

目的：用反转录PCR法，调取肝内胆管癌细胞内的端粒酶RNA基因，并针对其序列设计反义RNA封闭基因及锤头状核酶切割基因序列，并将其构建入真核表达载体pTriEx-4内。

The RT-PCR method can extract the intrahepatic bile duct cancer cell′s hTR gene.We construct the eukaryon express vector containing the antisense RNA and hammerhead ribozyme gene successfully.

RT-PCR法可调出其hTR基因有效片段；成功构建了针对hTR模板区的反义RNA基因和核酶基因的真核表达载体。

Distribution of ribozyme transcripts in transgenic potatoes showing strong resistance against potatoes spindle tuber viroid was also determined.

本论文还研究了高抗马铃薯纺锤形块茎类病毒的转基因马铃薯细胞内核酶转录产物的分布。

Using in situ hybridization, we found that ribozyme transcripts were predominantly located in the nucleus of transgenic potatoes, consistent with the hypothesis that ribozymes were more efficient against nuclearly replicative viroid.

原位杂交结果表明核酶转录产物主要分布在转基因马铃薯细胞的细胞核中。这一结果支持我们提出的核酶对在核内复制的类病毒更为有效的假说。

Potato spindle tuber viroid ; hammerhead ribozyme ; cleavage activity in vitro

马铃薯纺锤形块茎类病毒；锤头型核酶；体外切割活性

The genes of hammerhead ribozymes targeted against two and three sites on negative strand of potato spindle tuber viroid were designed, synthesized and cloned according to the action manner of hammerhead ribozyme.

根据锤头型核酶的作用模式，设计、合成并克隆了特异切割马铃薯纺锤形块茎类病毒负链RNA不同区域位点的双价和三价锤头型核酶基因。

The genes of hammerhead ribozymes targeting against two and three sites on negative strand of potato spindle tuber viroid were designed, synthesized and cloned according to the action manner of hammerhead ribozyme.

根据锤头型核酶的作用模式，设计、合成并克隆了特异切割马铃薯纺锤形块茎类病毒负链RNA不同区域位点的双价和三价锤头型核酶基因。通过体外转录，将PSTVd负链RNA分别与双价和三价核酶混合，37℃温育 2h。

ABSTRACT］ObjectiveTo construct a eukaryotic expression vector of M1RNA ribozyme against telomerase RNA and transfect to hepatocellular carcinoma cell lines.

摘要］ 目的构建抗人端粒酶RNA的M1RNA核酶的真核表达载体，并筛选稳定转染核酶的肝癌细胞株。

Objective To construct a eukaryotic expression vector of M1RNA ribozyme against telomerase RNA and transfect to hepatocellular carcinoma cell liness.

目的 构建抗人端粒酶RNA的M1RNA核酶的真核表达载体，并筛选稳定转染核酶的肝癌细胞株。

In recent years, Ribozymes and DNAzymes have been used in inclinical studies, and there have got many successful examples.

近年来，Ribozyme和DNAzyme在临床治疗研究中已经获得了长足进展，有许多成功的实例。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。