查询词典 ribozyme

Computer analysis of the cleavage reaction of hammerhead ribozyme ［J］.

核酶对靶RNA的体外切割反应的计算机分析［J］。

Computer was used to analyze the possible secondary cleavage sites on HPV11 E2 mRNA and to predict the secondary structures of substrate and ribozymes. According to the theory of Symons headhammer ribozyme, there are 32 sites to targetting sequences.

遵循Symons锤头状核酶结构和GUX剪切位点原则，靶序列存在32个剪切位点，通过计算机软件分析核酶的最佳剪切位点，并对底物及核酶的二级结构进行预测及进行相应基因生物学功能和基因同源性分析，筛选出2个锤头结构核酶。

HPV11 E2 mRNA was taken as the target RNA, ribozyme were designed accounting to the hammerhead structure described by Symons.

借助计算机软件分析，设计出能特异性切割HPV11型644nt E2 mRNA的核酶。

Intron removal is now thought to be an autocatalytic process in which the RNA acts as its own enzyme.

目前认为内含子的切除是一个由 RNA 自身催化（参见 ribozyme ）的过程。

Ribozyme can inhibit the expression of HBeAg in the cotransfected cells by cleaving the mRNA of HBV.

在核酶与HBV共转染细胞中，核酶通过对HBV mRNA的剪切作用，使HBeAg的表达量明显受到抑制。

The full-length genome cDNA containing genetic tags and flanked by Hammerhead ribozyme and Hepatitis delta ribozyme were cloned downstream of the CMV enhancer and the chicken beta actin promoter of the vector pCAGGS. After purified by Plasmid Midi Kit, the recombinant infectious clones named pCAGGmGtAHRT and pCAGGmGtBHRT were directly co-transfected DFI cells indued by Lipofectamine~ 2000. 72h post transfection. the supernatant of cell cuture was harvested and continue passaged in CEF cell. The results of indirect immunofluorescence.

分别在Gt株基因组两端引入了锤头状核酶结构(Hammerhead ribozyme，HamRz)和丁肝病毒核酶结构(Hepatitis delta ribozyme，HdvRz)，并将其克隆在真核表达载体pCAGGS的CMV增强子和β肌动蛋白启动子下游，构建IBDV感染性克隆pCAGGmGtAHRT和pCAGGmGtBHRT，经Plasmid Midi Kit纯化后，在Lipofectamine~ 2000的介导下，直接转染DFI细胞，72h后取上清继续在CEF上传代。

At the computers heart is a ribozyme – a short RNA molecule able to catalyse changes to other molecules.

计算机的核心是酶性核酸――一类具有催化断裂RNA作用的核酸分子。

We construct the eukaryon expression vector containing the tRNA-CTE hybrid ribozyme gene successfully which is the foundation for further study.

结论tRNA-CTE复合核酶真核表达载体的成功构建，为开展利用tRNA-CTE复合核酶切割HCV RNA的研究奠定了基础。

Aim To observe the effect of bcl-2 ribozyme on SMMC7721 cells and the expression of p16, p21,so to investigate the role of bcl-2 ribozyme in hepatocarcinoma treatment.

目的 观察bcl-2核酶对人肝癌细胞株SMMC-7721细胞的作用，并检测p16和p21的表达情况。

The c-erbB-2 oncogene-specific ribozyme has a high activity in vitro. It lays a foundation for the study of the therapeutic use of ribozyme in gene therapy of cancer.

结论c-erbB-2特异性核酶RZ1在体外有良好的切割活性，该实验为进一步研究其在肿瘤基因治疗中的作用奠定了基础。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。